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4- Bosela, M. J., and Michler, C. H. 2008. Media effects on black walnut (Juglans regia L.) shoot
culture growth in vitro: evaluation of multiple nutrient formulation and cytokinin types. In vitro
cell Dev. Biol. Plant. 44:116-121.
5- Driver, J. A. and A. H. Kuniyuki. 1984. In vitro propagation of paradox walnut rootstock.
HotrScience 19: 507-709.
6- Jay-Allemand C., P. Capelli and D. Cornu. 1992. Root development of in vitro hybrid walnut
microcutting in a vermiculite containing gelrite medium. Scientia Hortic. 51: 335-342.

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The 6th National Biotechnology Congress of Iran

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7- Kurz, W. G. W. 1998. Semi continuous metabolite production through repeated elicitation of

plant cell cultures: A novel process. In T. Y. Mabry (ed), Plant biotechnology. 93-103.
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(Juglans regia L.). J. Japan. Soc. Hort. Sci. 17:661-6

Micropropagation of Persian walnut (Juglans regia L.) by embryo

culture
M. Ferdowsi, M. Dana,S.Esmailzadeh *
*

Payame Noor University, Gonabad Centre

Abstract
One of the new and important ways of plant propagation is micropropagation or tissue culture technique.
Using this technique is preferred more due to its vast propagation of plants in short periods. This research
presents an investigation on media and culture conditions for in vitro proliferation of Persian walnut
(Juglans regia L.). Prepared embryo segments (explants) produced callous and new shoot and root on
WPM (woody plant medium) with and without the usage of IBA (Indole Butyric Acid) hormone. An
experiment was carried out under conditions of 25C, light/dark period of 16 and 8 hours and a light
intensity of 3000 lux. Results showed that root and shoot proliferation percentage on WPM supplemented
with 0.5mg IBA hormone were 70 and 60 percent respectively, whereas without using IBA the these data
were less than 50 percent. How ever, after the end of experiment about 60% of replications resulted to
seedling (completed plant). Comparing medium treatments indicates that the appliance of IBA improved
and promoted micropropagation of walnut.
Key words: WPM medium, Callous, Tissue culture, Micropropagation, Juglans regia