170 Poster Presentations ‘laborate arterial reconstruction was necessary du to multiple or polar arte tes. “Methods: Berwoen 1996 and 2008 111 consecuve living donor kidney trans- Pianlaions(LDKTX) were performed in our corte. Preoparatve evalvstion of the donors consist of detailed medical worcup, scniggatc evaluation of side pectic kidney function and CTMR-angiogralie to detest vascular aba ios. The key with ose functon and less artrios was selected for donation. Results: Polar areres were anastomosed to inferior epigastic artery (ne4) afer reconstruction of main renal artery with neither prclonged CIT nor WIT A substantial polar artery was anastomosed to internal lilac artery (9=1), equ alent damete of renal artanes were anastomosed in ostium cammune Tech iguo(n=1), Two cases presontod wth triple artorias which wore anastomacad via venous iterpostion ofa saphenal vein eogrnent. Operative time was eg rifeant longer wih need for arterial econstrucbon: 164 =: S8min vs 198 37 ‘nin. Moan CIT ir cases with arterial recontuction was 158 28 min ver ‘us 145 ++ 82 min in ats with single avtores, After reconstuclion al rats Soved iar fncton, pte of anes was document by dos ‘Conclusion: Vascular reconstructon of ational renal ators prolongs CT ‘ang operalive me. However as this does not harm primary function rate, r= ‘onettucion s wer itas nephron mass ean be saved. PO-527 ‘THE ANTIOXIDANT HYDROXYTYROSOL PROTECTS THE KIDNEY FROM THE CYCLOSPORINE INDUCED OXIDATIVE. ‘STRESS BUT NOT FROM THE HAEMODYNAMIC. ALTERATIONS Glovambatsia Capasso", Alessandra, Cantone', Chiara |. Di Gennaro? Valeria Miglaré?, Vineenzo Zapola’. 'Catedra d Netologia, Seconda Universita of Napod Napoli aly; *Blocbemisry, Seconda Universta di "Napol, Napoli ay Background: Cyciosporine A (CsA) Is tho frstino immunosuppressant usod in ranspiation and autornmune Gseasee, Nephvotovcty is he major lma- tion of CsA. Several evidences suggest that reactive oxygen spacis (ROS) play 2 role. This study simed to Investigate Pydroxjyrosal (DOPET) elect, fn antioxidant component of ova ol, an CeA-ndvoed oxidative sess and haemodynamic aferatons In art model of chvonle CBA nophotexc Methods: Sprague-Dawley rats were read ip. with CSA (15 gh) alone or in combination with DOPET (20 mghk) for 3 weoks. Glomerular fiation rate (GFR) wae assossod by inulin clearance; blood pressure was monitored by tha tall method. At he end, the animals wore sarfced and the superoxoe con entation within the abdomina aera and the renal atery cols was quantiied ‘rom the oxidation of dnydroatiium (OME) using fuorescence microscopic imaging anaiysi. On kidney issues, pc peroxidation was messuted as th barbiurc aci-reacing substances (TBARS), glutathione level by enzymatic ‘assay and heme oxygenase 1 (HO-N mRNA exprocsion by semiquantitative RTPCR, Results: CsA increased superoxide concentration inthe aorta and in tho renal artery; DOPET inhibited tis effect. CSA-reate rata showed highorlovels of "TBARS in hor kinoys, a docreace of GSH and HO-1 mANA up-regulation in ‘comparson with contois. DOPET reversed these CsA effects, Aer CsA trea ‘ment GFR decreased and biood pressure increased; DOPET ci not improve ‘renal haemadynamios and hypertension, Conclusion: DOPET protecion rom CsA induoed oxdatve stress snot ass ated witha paral action on haemodynamics. Therelore free redcals sear ‘not tobe completely responsibe forthe haemodynamic CsA toxicity INCIDENCE OF CARDIOVASCULAR RISK FACTORS AND COMPLICATIONS BEFORE AND AFTER KIDNEY ‘TRANSPLANTATION Mohammad Ali Ostovan,Alteza Mebdizadeh, Atsoon Fazolzader, ‘Al Razmkon, Ganbar Al Raiseeal, Seyed Ali Malok Hossein, Nomazee “Hospital Shiaz Tansplent Research Coniar, Shiraz, Fars, slae Ropubc ofan. Puspose: Cardiovascular diseases a leading cause of deat ater renal rans- plantation (FT), an the incidence fs eonsieraby higher tan in the general Population. Aim ofthis study was to evaluate the incidence of atherosclerotic ardiovascular completing ater PTs. Methods: Anayss of ahoroscoroc cardiovascular cisoasos and cariovas- Cur isk actors before and afer ransplantation In 500 renal Yansplant recp- Torts batwoon 1967 ans 1992, Results: Following RTx 11.7% developed atheroscertle cardiovascular ds- ‘260s, the malory coronary artry disoaso (9.8%). The comparison ot risk ‘actors before Yanspiantation showed: The prevalence of system typertan ‘son (67% to 86%), diabetes melitus (7% fo 16%) and obesity (20% to 48%) had increased signiicanty whereas the rumor of smokers halved 10 20%. “The tigheendes decreased significantly (235 © 144 to 217 + 122). The to and HDL cholesterol rose sigifoanty (282 + 65 to 278 + 62 and 47 + 29 PO-528) 1058 4 21, respectively). The LDL cholesterol increase was naga 12 62 to 16) + 58) nthe univariate analyia,carbovascar disease ‘Snifcantyassociatod wth mate gender, age over 50 years, laboes (OM), smoking total cholesterol > 200, LDL cholestara ~ 180, HDL terol 55, fbrinogen = 950, body mass index > 25 kg/n, and more anthyperionsve agents per day Conclusion: The high incidence of cardiovascular disease folowing traneplanaton ie mainly duo o a high prevalence of classical rik facto fore and folowing ransplantaton. The treatment ofthe sk factors mus introduced eary in the course cf rena allure, further, they must be folowing tansplantaion. PO-529 | PRETRANSPLANT CARDIAC INVESTIGATIONS INTHE = IRANIAN RENAL TRANSPLANT POPULATION-THE, EFFECTIVENESS OF OUR CURRENT SCREENING ‘TECHNIQUES IN PREDICTING CARDIAC EVENTS ‘Mohammad At Ostovan,Alroza Mehdlzadeh, All Razmkon, ‘soon Fazeizadeh, Gantar Al Ralsslal, Syed All Maisk Hossein Nemazee Hospital, Shiraz Tanspisnt Research Genter, Shiraz. Fars, ine eputle of ran, Purpose: Coronary atory disease (CAD) is provalont among onc-stae ‘al falure paten's and remains the major cause of moray flowing ret transplantation. Death witha functioning transplant intitute reins theme ‘common cause of Kidney gat fare n this study we atom o eva he feflectiveness ofthe clinical history and current sotsring tocniques aaa ‘n predicting postransplant CAD and also ascees the rle of coronary ag ‘raphy a8 a pretransplant screening technique ‘Methods: Ciical data of 190 renal ranepant patents was analyze. Anyi ‘cal history ofeaciac disease and all preoperative cardiac screening datas ‘ecorded for each patent. The study endpoints were the subsequent de ‘opment of myocardial infarction (Ml), undergoing coronary artry Bypass Gat (CABG) or death, Feesults: Factors that were significantly associated with reaching a study en ‘oln included: age at tanpans [Hazard Rao (HR) 1.91, P=0.001], Noro hart flue {HR 8.22, P 0.001), prosence of CAD on coronary angio (HF 5.55, Po0.033), artoror @ wavo on electocaréograph (ECG) (HABA P-<00001) and history of a cerebrovascular accident (HR of 4.2, P=0.008 “The seaning techniques of myocardial pertusion ecan and echocarcograaty wore conclusive as prdietive variables of outcome. Conclusion: Cincal history, ECG recuts and echocardiography are gat practical and low-cost screening metods. Coronary anglograpry s appop ate In certain righ groups but not necessary as part of screening ina poten renal transplant recipients, PO-530| ELEVATION OF MONOCYTE CHEMOTACTIC PEPTIDES. (MCP-1) AND ACTIVATED CELLS IN URINE - SENSITIVE [REJECTION MARKERS IN RENAL GRAFT RECIPIENTS. ‘Bogdan Dubinski", Maria Boraynsia’, Wactaw Kopec', Maran Klinger’ rot Szyber®, Dariusz Patrzalek*, Department of Nephrology, Wroclaw ‘Medical University, Wroclaw, Poland: "Deparment of General, Vascular ard Tansplant Surgery, Wroctaw Medical Unversity, Wroclaw, Poland Cytokines produced during _soute immunological alloregponse indus Chemokines what results nth ifitraton of the alloea. These chemokines ‘and cals re excreted with urine. The alm ofthe study was to assess the g- Paste utlty of unary excretion of MCP-1 end CDS+, CDid+ and HLA-DR col, ‘Tho study entalod 35 patents with acute renal rejection and 6S wit state galt function. Unnary Sediments, were propared by moans of cyiospn and Stained with an-CD3, ant-CD14, ant-HLA‘DR labeled monoclonal antibod les and expressed as counts per 10 high power felts. Urinary MCP-1 was assayed by ELISA and expressed as ng per mg of creatinine. In pationte with acute rejection MCP-1 level was terol higher than in pa ints with stable graft function (6.1343.38 vs 0.6020.35 ngimg creatinin) ‘The number of CDS+ cols was approx. four times higher than in the non rejection patents (0.04.6 vs 2.02.2), The number of DRe cols was 8 times higher inthe acute ejection pallets (18.027.2 vs 20327). The counts of (OD3+ and DR cols correlated with Ban score. ‘The asesssment of MCP-1 28 wel as CD3s and HLA-DR celle can provide & Useful nonimasive davie for elagnosis of acute rejection. The numberof COrs ‘eis was sgnticaty Increased in pation during acute rejection (p=0.000"), (63, DR+ and CDi4 cell counts stonaly corelated wih urine excretion ct MCP: