Stem Cells in Regenerative Medicine: Carpe Diem – Carpe Vitam!

Copyright © 2019 Dr Mike Chan and Dr Dmitry Klokol

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Dedicated to all cell therapists and stem cell researchers who have devoted their careers to improving people’s quality of life and fighting diseases and aging

Your time is limited, so don’t waste it living someone else’s life. Don’t be trapped by dogma – which is living with the results of other people’s thinking. Don’t let the noise of others’ opinions drown out your own inner voice. And most important, have the courage to follow your heart and intuition.

Steve Jobs

Everyone here has the sense that right now is one of those moments when we are influencing the future.

Steve Jobs

When something is important enough, you do it even if the odds are not in your favor.

Elon Musk

Nature loves courage. You make the commitment and nature will respond to that commitment by removing impossible obstacles. Dream the impossible dream and the world will not grind you under, it will lift you up.

Terence McKenna

We are asked by science to believe that the entire universe sprang from nothingness, and at a single point and for no discernible reason. This notion is the limit case for credulity. In other words, if you can believe this, you can believe anything.

Terence McKenna

Medicine is not only a science; it is also an art. It does not consist of compounding pills and plasters; it deals with the very processes of life, which must be understood before they may be guided.

Paracelsus

Look deep into nature, and then you will understand everything better.

Albert Einstein

The good physician treats the disease; the great physician treats the patient who has the disease.

Medicine is a science of uncertainty and an art of probability.

It is much more important to know what sort of a patient has a disease than what sort of a disease a patient has.

William Osler

Stem cell research is the key to developing cures for degenerative conditions like Parkinson’s and motor neurone disease from which I and many others suffer. The fact that the cells may come from embryos is not an objection, because the embryos are going to die anyway.

Stephen Hawking

CONTENTS

Foreword

Introduction: Xenogeneic Cell Therapy In Regenerative Medicine

PART 1 GENERAL CONSIDERATIONS ON STEM CELLS

1.Understanding Stem Cells

From embryology to regenerative medicine

History of stem cell research

Stem cell biology

Choice of stem cells in wellness and biological medicine

2.Preparation, Standardization, Laboratory Animal Care, Safety Protocols, and Regulatory Aspects of Stem Cells, Xenotransplants and Biomolecular Therapy Products

Animal sources of stem cell xenotransplants

Tissue procurement and cell culturing

Archiving of medical records and specimens from animal sources

Quality control

Release and labeling of precursor stem cells

3.Genetic and Immunological Aspects of Cellular Xenotransplantation

Genotype-phenotype distinction and relationships between donor cells and recipient

Epigenetics and stem cells

Immunology and genetics in stem cell therapy

4.Safety Considerations of Fetal Xenogeneic Precursor Stem Cells

5.Stem Cells’ Biochemical Basis and Mechanisms of Action

Homing and migration of fetal precursor stem cells

Adhesion, binding and interaction of fetal precursor stem cells

Paracrine mechanisms of fetal precursor stem cells

Soluble paracrine factors of fetal precursor stem cells

Insoluble paracrine factors of fetal precursor stem cells

Further considerations on the immunogenicity of fetal precursor stem cells

6.Autologous Stem Cells and their Therapeutic Potential

Autologous stem cells and history of their transplantation

Autologous stem cell transplantation

Very small embryonic-like stem cells (VSELs)

Clinical applications of autologous stem cell transplantation in different diseases

Currently available treatments for secondary progressive multiple sclerosis (SPMS) have limited efficacy and/or safety concerns

Criticism of VSELs

PART 2: PRECURSOR STEM CELLS IN THE TREATMENT OF NEURODEGENERATIVE DISORDERS

7.Morpho-Physiologic Background and Substantiation of Precursor Stem Cell Therapy use in Neurodegenerative Diseases

8.Cell Therapy in the Management of Autism Spectrum Disorders

Outlook on the etiology and pathogenesis of ASD: role of genetics and immunity in development of autism and ASD

Role of imaging studies and biological markers in autism and ASD and their links to symptomatology

Integrative therapeutic protocol for autism and ASD

So what will adequately address biological parameters and the needs of the child with ASD?

9.Cell Therapy in the Treatment of Alzheimer’s Disease

Social and general medical aspects of AD

Therapeutic strategies in AD

Stem cell therapy in the treatment of AD

Mito Organelles™ CNS Neuropeptides in the management of AD

10.Cell Therapy in Parkinson’s Disease

Social and general medical aspects of PD

Protective factors against PD

The role of mitochondria in PD

Clinical forms of PD

Three stages of PD diagnostics

Classification of degrees/levels of PD severity

Clinical diagnosis of PD

Treatment strategies in PD

Treatment of PD with precursor stem cells

Neuropeptides Mito Organelles™ the CNS in management of PD

Transcranial electric and magnetic stimulation of the brain (TCS)

11.Cell Therapy in Stroke Management

Social and general medical aspects of stroke

Risk factors and symptoms of stroke

Post-stroke rehabilitation

Stem cells and cell therapy in management of stroke

Mito Organelles CNS Neuropeptides in the management of stroke patients

PART 3: PRECURSOR STEM CELLS IN THE TREATMENT OF SYSTEMIC DEGENERATIVE DISORDERS

12.Cell Therapy in the Management of Diabetes Mellitus

Morpho-physiology of pancreas and general medical aspects of diabetes mellitus

Stem cells in the management of diabetic patients

Nutritional intervention and change in lifestyle

13.Cell Therapy in the Management of Cardiomyopathy

Pathogenesis of cardiomyopathies and cardiac regenerative potential

Cell therapy as a treatment modality in cardiomyopathy

14.Cell Therapy in the Management of Chronic Kidney Disease

Mechanisms of kidney regeneration

Stem cells in kidney regeneration

Stem cell xenotransplantation in the management of CKD

15.Cell Therapy in the Management of Liver Disease

Pathogenesis of liver disease

Cell therapy-based treatment of liver disease

16.Cell Therapy in the Treatment of Muscular Dystrophies

General medical considerations on muscular dystrophies

Pathogenesis and diagnostics of muscular dystrophy

Novel treatment strategies in muscular dystrophy

Immunotherapy in MD

Stem cell-based therapies in the treatment of MD

17.Cell Therapy in the Management of Retinopathy

Retinal morphology and pathogenesis of retinopathies

General considerations of cell therapy-based treatment strategies in retinopathy

Bioengineering in clinical trials using human pluripotent stem cells

Precursor stem cell/live cell therapy in retinal diseases

Differentiation of hESCs and hiPSCs into three-dimensional retinal tissues

Cell-based therapies for retinal pigment epithelium replacement

Cell-based therapies for photoreceptor replacement

18.Placental Therapy as Part of Cell Therapy and Regenerative Medicine

Morphophysiology of the placenta and physiological background of placental therapy

Main characteristics of placental therapy products

Biological and medicinal properties of placental extracts

Placental therapy in the treatment of hair loss

FOREWORD

In our previous book, A Comprehensive Guide to Biological Medicine and Wellness, we spoke about a general approach to holistic biological medicine and maintenance of well-being. We presented our vision of therapeutic paradigms of biological medicine, its steps, namely Diagnose, Detox, Repair and Rejuvenate, and the prime treatment methods that we recruit in order to attain wellness in our patients. We did touch on certain aspects of regenerative medicine, though concisely, and now we feel that it needs to be further elaborated on. But before you proceed with reading the entire book or its chapters we would like to propound some justifications of why one should do so.

So, why should you persist in reading this book? To justify it, we should unravel a few concerns, such as What is regenerative medicine and is it feasible at all? and Is it possible to regenerate the body and/or tissues?

To regenerate an organ or tissue literally means to grow new tissue after its loss or damage. The key to understanding how living organisms and tissues are generated lies in embryology. In nature, all biological tissues arise from stem cells. A stem cell is an undifferentiated cell that has the capacity to replicate itself and give rise to infinitely more cells of the same type, continuously maintaining its existing pool and also able to differentiate into any type of cell in the body. In science it is described by the terms self-renewal and potency. It defines the natural universal mechanism of all living beings, including the human, to grow and to self-repair. A newborn organism has essentially a higher ratio of stem cells per volume of tissue and as it grows and starts to age the amount of stem cells drops. That process is directly related to a decline in the ability of tissues to regenerate themselves, which is even accelerated by such unfortunate events as trauma or disease. Therefore, the bottom line is that regeneration of the tissues is impossible without stem cells being the core element in this process. Consequently, regenerative medicine implies either stimulation of the existing pool of stem cells (intrinsic) or introduction of new stem cells into the organism (extrinsic).

Over the last few decades we have heard all sorts of narratives about stem cells to the extent that the term stem cells has become relentlessly abused. Despite the overwhelming data about stem cells, most doctors are still unaware of, or at least unclear about, what stem cells in fact are and how they can be utilized. Indeed, the topic of stem cells is still a gray area, both in the eyes of authorities and the minds of doctors. The initial euphoria about embryonic stem cells has turned into major disappointment and ended up as an abandoned ship on the reefs of biological unacceptability and unpredictability. Autologous stem cells, so appealing in their availability, carry more limitations than therapeutic potential. Fetal precursor stem cells, scientifically and theoretically most appropriate, however, are not easy to obtain. Our global research in biology and biotechnology is developing so fast that we may think we know most of the answers. Then why is regenerative medicine – and stem cells – still not mainstream medicine yet? Why do most scholars still refer to it as medicine of the future? The main hurdles of stem cells are ethical, moral and legal, and also practical. How, and from where, to procure stem cells? How to culture them, expend their volume and keep them alive? How to introduce them into a patient’s body, deliver them into the tissues? How to escape the immune response of the host? How to handle, store and transport them in vitro? And much more…

The science of stem cells was developed on both sides of the Atlantic Ocean, however, European doctors, from Eastern Europe, Germany and Switzerland, were more daring and successful in their clinical application. Various forms of cell therapy have been successfully practiced in Europe for a hundred years to date. From Küettner and Niehans to Franz Schmidt, Albert Landsberger and Carl-Gustav Groth… Thousands of therapists – millions of clinical applications.

Hence, the answer to the first question is: No, regenerative medicine is not a hoax, it stands on the undeniable scientific fundamentals and countless historical examples of successful applications.

Brothers-in-arms: Prof. Mike Chan with Prof. Albert Landsberger.

Here we come to the second question. The question of the credibility of the authors of this book. The team of contributing authors is an amalgamation of clinical doctors with research and educational backgrounds’ and cell biologists and researchers in the field of cell therapy.

Prof. Mike Chan started his research in cell therapy in Switzerland and Germany in the 1980s. Over the years, among his mentors have been some of the top renowned cell therapists in Germany, including the late Prof. Albert Landsberger.

Prof. Mike Chan and his team traveled across many continents advocating research on stem cells and providing education for doctors all over the world. In a way, it can be said that cell therapy had been made affordable and open to medical communities and patients in Asia and other parts of the world due to Mike Chan, his wife and partner Prof. Michelle Wong and their dedicated team. Nearly 35 years of effort and endurance was invested into the research and development process, laboratory work on stem cell culturing, the quest for possible practical applications, and education, education, education.

Our endeavours found recognition among many peers, scholars, practitioners and officials in many countries. And of course thousands of grateful patients whose lives were dramatically improved with cell therapy. So, we have answered the second important question.

Before you proceed with reading this book, we would like to remind you that the more we learn and discover, the more we find that there is much more we don’t yet know or understand. But it is worth trying.

For the sake of the future, for the sake of progress, for the sake of science, for the sake of mankind. Particular aspects of stem cells and cell therapy are hypothetical, still in the research and development stage; much is still unknown or unexplained. And yet continuing the pursuit is vitally important. Stem cells and cell therapy can be compared to a leap of faith – you have to believe in it in order to succeed!

With all sincerity and good heart we invite you, dear reader, to join our Movement of Believers in Regenerative Medicine.

Main authors,

Mike Chan and Dmitry Klokol

Prof. Dr. Anam Kour

MD, PhD

When I was first introduced to this newly published book in 2019, I experienced that eureka moment – that That’s it - I found it moment after years of practicing age management and regenerative medicine. It has been invaluable in my daily clinical practice. It is a reliable authority in this evolving and expanding specialty branch of frontier medicine. It has become a constant reference material since.

That’s it - I found it

Being in medical practice the last 41 years, I reflected back on some of the major medical advances of the past decades. In December of 1954, Dr. Joseph Edward Murray of Boston, performed the first truly successful human kidney transplantation. Thirteen years later, in December of 1967 Dr. Christian Barnard of Cape Town carried out the first human to human heart transplant. These were significant medical breakthroughs in medicine, and herald a new bold field in human whole organ transplantation.

In 1981 two scientists, Martin Evans at Cambridge, and Gail Martin in California, in their separate research, derived pluripotent stem cells from mice embryos – the first embryonic stem cells ever to be isolated. Seventeen years later, in 1998, James Thomson and Jeffrey Jones created the first batch of human embryonic stem cells, derived from early embryos. These again were significant advances in medical science that paved the way for stem cell therapy in subsequent years.

This new field of stem cell biology and regenerative medicine is neither taught in medical schools nor is the knowledge available in mainstream medicine. Current practicing doctors, be they general practitioners or super specialty specialists, are neither taught nor exposed to stem cell medicine. They oftentimes read cursorily at the periphery or gather bits and pieces of information from the media. Hence the time is ripe for a book as this to be presented to the medical fraternity.

This book, Stem cells in regenerative medicine, is structured, organized, comprehensive, and provides a syllabus for learning. It is a ready source of knowledge and reference materials. Readers will appreciate the latest research materials that were incorporated and the new clinical applications across the spectrum of diseases. This book will set the standard against which other future publications will be judged

Contemporary medicine is focused on pathological diagnoses followed by disease eradication. For chronic diseases i.e. diabetes mellitus and hypertension, it is maintenance and damage control. Stem cell therapy has a different focus. It is a new dimension in treatment – that of repair of sick cells, reversal of degenerate cells, and regeneration of lost cells.

This book is aimed at practicing clinicians, be they specialists or general practitioners. Regardless of their interest in stem cells, each practicing physician should have a personal copy, without which, these doctors risk being left behind. It is hoped that this book will pique the interest of many clinicians, leading and guiding them to offer a higher level of care to their patients.

I congratulate the authors for this magnificent and timely book that the medical fraternity, knowingly or unknowingly, has been waiting for.

Professor Dr. Anam Kour, MD, PhD

Orthopedic Surgeon

Royal College of Surgeons Glassgow

University of Singapore

University of British Columbia, Canada.

Dr. Desirée Cox

MD, PhD

The history of medicine is full of examples of innovators seeking to create new therapeutic approaches to help patients achieve health and wellness. Stem Cells in Regenerative Medicine: Carpe Diem – Carpe Vitam! by Mike K. S. Chan and Dmitry Klokol presents a natural history of the evolution of an approach, which they’ve developed over close to three decades, of integrating the best Western or ‘European’ treatment modalities with certain components of traditional Asian medical systems. ‘European Biological Wellness’ is the term developed by the authors to describe an approach characterized by a four-step signature algorithm – ‘Diagnose, Detox, Repair, and Rejuvenate’ or DDR – in which stem cell therapy forms a core component of their therapeutic approach.

making invaluable contributions to a broad spectrum of medical-related audiences and the future of health.

The promise of cell reprogramming has long been the holy grail of medicine. It captures the core essence and almost mythical potential of the ‘fountain of youth’ whereby, as human beings, we are able to ongoingly heal our physical body and ourselves. In many ways, the field of stem cell and regenerative medicine captures the imagination of the public because of its potential to deliver on this promise.

In my personal view, the field of stem cell and regenerative medicine in its current state has some way to go before it fulfills its potential. That said, regenerative medicine is rapidly evolving, driven as much by critically important global safety and efficacy regulatory requirements as it is by the shifting landscapes of science and technology, not to mention public education and awareness. In recent times, questions as fundamental as ‘what is a stem cell?’ have come under scrutiny and are hotly debated in certain scientific circles. Indeed, I believe we will likely see a revision and re-defining of many terms, definitions, and hypotheses as we move towards a more unified, interoperable system and codex of the regenerative sciences.

As founder and CEO of The HEALinc and The HEALinc Future Health Innovation Summits, I believe that during this period of accelerated evolution it is important to learn from and about the perspectives of innovators in stem cell and regenerative medicine across the globe. A Unified Sciences of Regenerative Health that offers patients safe alternatives for their healing process will require the wisdom of many healthcare providers, innovators, entrepreneurs, and more.

By sharing their extensive experience and knowledge of close to three decades in the regenerative medicine field – and by outlining the natural history of their approach in A Comprehensive Guide to Biological Medicine and Wellness and Stem Cells in Regenerative Medicine: Carpe Diem – Carpe Vitam! – innovators like Professor Mike Chan and Dr. Dmitry Klokol are recognized as pioneers in the field, making invaluable contributions to a broad spectrum of medical-related audiences and the future of health. It is critical that Professor Chan, Dr Kolkol and the European Wellness team use the descriptive account of the DDR algorithm and the clinical experience of stem cell therapy outlined in this book form the basis of more intensive scientific research and clinical trials to aimed at advancing the field.

Dr. Desirée Cox, MD, PhD

Oxford University - UK

Cambridge University - UK

McGill University, Canada

Recipient of the 2019 International Regenerative Medicine Action Award at the World Stem Cell Summit, Miami, Florida, USA.

http://vaticanconference2018.com/wp-content/uploads/2018/04/Fourth-International-Vatican-Conference-Agenda.pdf.

For the panel session with Dr. Cox (April 27, 2018), see: https://www.youtube.com/watch?v=a4gdAWTrLGM&sns=em

Dr. Orn Adalsteinsson

I was totally intrigued

The Stem Cells in Regenerative Medicine Book is an outstanding presentation of what can be achieved in novel therapies that involve cellular and non-cellular biologics with targeted approach for personalized human health improvements. The book authored by Prof. Mike Chan and Dr. Dmitry Klokol provides numerous case studies that show how stem cells as integrated with their four step personalized clinical protocol – DDRR (Diagnose, Detox, Repair, Rejuvenate) – can provide for advanced achievements in difficult to treat human diseases that the conventional medicine has not been able to touch.

cellular and non-cellular biologics for human health.

I had the privilege of meeting with Prof. Chan and Dr. Klokol in 2018 at a technical conference in San Diego, where I got my first introduction to their work. I was totally intrigued to learn that Prof. Chan and his wife Dr. Michelle Chan have been, for the past 35 years, pursuing their technology dream of using cellular and non-cellular biologics for human health. The effort has resulted in a technology empire that is being applied at numerous countries around the globe.

The Stem Cells in Regenerative Medicine book is their latest book with multiple case studies demonstrating the breakthroughs that can be achieved. The book provides details of the strong regulatory components of their stem cell efforts. All of their cellular biologics are produced in GMP (Good Manufacturing Practices) compliance facilities where the materials can be reproducibly manufactured with the guarantee of being void of multiple specific diseases. Due to the increasing global emphasis on regulatory requirements, Team Mike and Dimitry is embracing the opportunity to intensify their scientific efforts including clinical studies and expansion into the peer reviewed publication field.

Dr. Orn Adalsteinsson

Chief Scientist, Life Extension - US

President, ISCA (International Strategic Allia)

(MIT - Massachusetts Institute of Technology, USA)

Dr. Michael Papacharalambous

MD, PhD.

As chief physician in Metropolitan hospitals (approximately 1500 beds) in Greece and serving as General Secretary of the European Society of Preventing and Regenerative Medicine (ESAAM) responsible for the clinical applications in regenerative medicine we were working on how to generate healthy cells to replace diseased cells. In theory stem cells can be guided into becoming specific cells that can be used to regenerate and repair diseased or damaged organs and tissues in persons.

I recommend this book to be read by all clinical physicians and scientists in biology

From the book I understood why stem cells that come from bone marrow or blood in transplant procedures to treat diseases of blood cancers are successful and why trying to treat advanced degenerative nervous system diseases as Multiple sclerosis, Parkinson’s or Lou Goering’s with stem cells from skin or fat had partial or no success.

I recommend this book to be read by all clinical physicians and scientists in biology in depth it has all the information they need for a successful journey in the medicine of future.

It has all the information they need to understand from basic principles to advanced technology and education. I am happy to collaborate with Professor Mike Chan in Our ORTHOBIOTIKI clinic in Greece and implementing regenerative protocols.

Dr. Michael Papacharalambous MD PhD

Medical Director / Chief Physician

Metropolitan Hospitals, Greece

General Secretary / Board Member ESAAM

(European Soceity of Preventive, Regenerative & Anti Aging Medicine)

Her Holiness Sai Maa

MD

Mike is very devoted, dedicated, service oriented, ethical, humble, high quality and high energy. He is like a chef d’orchestre, the way he sees substance and creates it. He creates masterpieces. He is so passionate, and his style of work resonates with my passion and work to serve humanity to be happier and healthier.

He is someone who can bring the light of each cell in the physical body to surface.

He is like an artist of scientifically grounded information, of life extension as an experience. He likes exploring on a cellular level and structure. He is one who can really guide the stream of medicine right now, for the medical community to shift from an old medical paradigm to the next level, to see patients as whole, holistic in its full spectrum. He is someone who can bring the light of each cell in the physical body to surface. For someone as myself who is always looking for efficacious solutions to live a longer, gracious, fulfilling life expression, solutions that are cutting edge medically, scientifically and esoterically with all the modalities that I see in myself, I appreciate that about Professor Mike Chan.

I appreciate his entrepreneurial mind, youthful and visionary, using protocols as I used to when I had my practice: being bold on a cellular level and structure. He understands the quantum behind everything else. He represents for me an example of the new medicine system, new medicine protocol. The beauty of him is that he has the power to bring the paradigm of the new diagnosis, the new detox system, the repair, the rejuvenation, the regeneration, the hormones, all advanced techniques from dark field to high level of colonics, IVs, nutrients, hyperbaric, any device including the subtleness of peptides work in all its variety. He is the example of the real medicine in its full spectrum, all the way from organs and glands to neurogenerative.

Her Holiness Sai Maa, MD

https://www.sai-maa.com/en

https://www.sai-maa.com/en/about-sai-maa

https://en.wikipedia.org/wiki/Sai_Maa

Michelle Hughes

Early in my Life, I recognized that one of my passions is to inspire and influence others to be the best version of themselves and for me to be a teacher, influencer and role model for health and well-being. Imagine my delight when I was introduced to the CEO of European Wellness, Professor Dr. Mike Chan, and his international Group Medical Director, Dr. Dmitry Klokol, and to their exciting new books(*1).

Their collaboration is a masterpiece of scientifically grounded information, and of their life long exploration and experience aimed at teaching and guiding the medical community and the lay person who are looking for efficacious solutions to living a longer and healthier life using cutting edge scientific and aesthetic modalities. Prof. Mike Chan is a tireless, humble entrepreneur and visionary whose youthful energy and accomplishments are proof that his protocols work! Anyone who tries their therapies and products, and visits any of their 26 centers, can attest to their dedication to quality and service.

Their collaboration is a masterpiece of scientifically grounded information

They say there are no accidents in life, and for this happy accident of interaction with these two fine gentlemen, I am eternally grateful.

Michele Hughes

CEO, Real estate entrepreneur, Investor and podcast host of Ageless and Timeless

Michele is a serial entrepreneur. Her most recent career is in luxury residential real estate development, primarily in resort locations such as Aspen, Kauai, Cabo San Lucas, Idaho, Las Vegas and San Francisco. Her company grew from start up to multi million dollars in assets with both custom homes she built and raw land in her portfolio.

Sunny R. Kim

MD, FAAPMR

The pearls of clinical wisdom in this book are made possible owing to the success of like-minded pioneers in regenerative medicine.

We should all be reminded that the fundamental unit of life is the cell. Therefore, it stands to reason that a biological basis to treating disease and maintaining wellness is a fundamental pragmatic approach in medicine. Unfortunately, this approach has been foreshadowed by the growth of a molecular pharmacologic lock and key paradigm driving industry which all too often fails to address the crux of the problem. Conventional pharmacologic approach often fails to address the complexity of multivariate biological systems which need to maintain homeostasis in order to achieve optimal health. Blocking a signal at one level often leads to a disruption downstream which we observe as side effects. When we address disease at the cellular level, we tap into the human body’s remarkable blueprint for healing while restoring homeostasis and avoiding unwanted complications. Through the rational application of cell based technology, signalling molecules, and energetics we are able to achieve the unthinkable in medicine. The pearls of clinical wisdom in this book are made possible owing to the success of like-minded pioneers in regenerative medicine. Through decades of research and real world practical applications on a global level, this book brings to the forefront, a much needed refresher in medicine that the practicing physician will find intuitive and inviting.

To your health,

Sunny R. Kim, MD, FAAPMR

Founder and President

Progressive Rehabilitation Medicine, P.C

Cedar Rapids, Iowa

Diplomate American Board of Physical Medicine and Rehabilitation Associate Professor of American Academy of Stem Cell Physicians

Dr. Mulyadi Tedjapranata

DTM&H, MTh.FIAS

Prof. Mike K.S.Chan and Dmitry Klokol has written a great book that share with you Stem cell in regenerative medicine: Carpe Diem- Carpe Vitam! This book reveals concept and protocols of successful approach to regenerative medicine by means of stem cell therapy. Apply the principles of the book and you will be able to achieve exceptional results in the many areas of your knowledges. I salute the great efforts of both of you to empower the millions of people all over the world.

Dr. Mulyadi Tedjapranata DTM &H, MTh.FIAS

I salute the great efforts of both of you to empower the millions of people all over the world.

Prof. Dr. Deby Vinski

Msc, PhD

It’s a great book!

As an ingroup member of Regenerative Medicine and Stem Cell Profession, we have obviously digested lot of professional literatures in these fields. The book of Prof. Dr. Mike K.S. Chan & Prof. Dr. Dmytro Klokol however, contains new approaches and values. The systematic approaches made it easy to uncover stem cell and regenerative medicine. The contributors deserve thumbs up for their works. It fit nicely with the motto Carpe Diem - Carpe Vitam toward the new roles of regenerative medicine and Stem Cells. It’s a great book!

Prof. Dr. Deby Vinski, Msc, PhD

President of World Council of Preventive, Regenerative and Anti Aging Medicine (WOCPM)

new approaches and values

Dr. W. Andralia Kartolo

MSc (UK), MD

Prof. Dato’ Sri Dr. Mike Chan has never stop amusing me and the whole world in Antiaging Society. His works are always so profounding and mind-blowing. Thank you for sharing your magnificent creations with us. It has been an honour to write a sincere foreword to your Stem cell, Biological wellness and Cell membrane books.

Dr. W. Andralia Kartolo, MSc (UK), MD

profounding and mind-blowing

Dr. Cathleen Gerenger

It is with great pleasure that I write this foreword for Professor Mike Chan. It is an honor and privilege to share my respect for Professor Chan’s determination to pursue cell therapy research and teach the world what he has learned in the regenerative therapy arena. Professor Mike Chan’s tireless research holds the foundational key to unlock your body’s health potential.

a great opportunity for doctors, health care providers and medical schools to incorporate in their medical practices and medical school curriculum.

My name is Dr. Cathleen Gerenger. I have been in private practice in Florida, USA for over twenty years. I received my doctorate in Chiropractic Medicine at the age of twenty-four. My love for nutrition, hormonal health, and regenerative medicine led me to connect with Professor Chan. I had the pleasure of meeting him during my International Speaking Venue in Malaysia. Professor Chan and I clicked immediately when we shared our passion regarding regenerative medicine. Professor Chan invited me to visit European Wellness, in Kota Kinabalu, Malaysia, one of the 26 clinics he is involved with. I was impressed by the professionalism of his team and witnessed the meticulous details his well-trained staff provided when it came to patient care. When I returned to the United States, still inspired by my observations, I started to implement Professor Chan’s protocols into my clinical practice.

Professor Chan and co-author Dr. Dmitry Klokol have written several books. Stem Cells In Regenerative Medicine – Carpe Diem – Carpe Vitam. is a must read! I feel that the wealth of knowledge this book provides will be a great opportunity for doctors, health care providers and medical schools to incorporate in their medical practices and medical school curriculum.

Thank you, Professor Mike Chan for your friendship, zest for life and above all, your love for humanity.

With great respect,

your inspired friend,

Dr. Cathleen Gerenger

DC, FIAMA

RaJeunir Wellness, owner

Greenite, LLC, owner

UCARI, LLC, partners

Prof. Dato’ Dr. Chin Yew Sin, JP

The history for a human being to quest for a immortality elixir that could enable him to live forever dated more than 2,200 years ago.

Qin Shi Huang was the founding father of the Qin Dynasty and the first emperor of a united China. From 247 to 221 BC he was the King of Qin State. He became China’s first emperor when he was 38 years old after the Qin State had conquered all other Warring States and unified the whole of China in 221 BC.

After he had founded the Qin Dynasty, he built the Great Wall, he wanted his empire to last forever and he wanted to live forever also. Newly discovered documents shown that 2,200 years ago, he even issued an executive order to look for a elixir that would enable him to have an eternal life.

It is not a dream anymore to live beyond 100 years old!

He sent Taoist alchemist Xu Fu to the eastern seas with 500 young men and 500 young women to quest for the elixir in the legendary Penglai Mountain, but returned without getting it. Xu Fu set sail on a second voyage with 3000 young girls and boys, but they never ever returned, instead, Xu Fu found Japan.

That was the history for human beings to search for elixirs for immortality a few thousand years ago, however, the quest never stops there since then until now. The dream for a human being to live forever or at least to live as long as possible was/is a common desire of all human beings in the past and present time.

With advances achieved in the field of Stem Cell Technology recently, it is not a dream anymore for a human being to live beyond 100 years old! We don’t have to go too far away places to search for the immortality elixir as compared with the Qin Shi Huang’s time, we just have to look for Prof. Dato’ Sri Dr. Mike Chan, an International Renowned Stem Cell Expert, he will be able to make you look younger, healthier and live longer also!

Stem cells in particular, Precursor (Progenitor) Stem Cells or Progenitor cells are the most vital cells that keep our organs active from degeneration with age as they are targeted at specific organs.

After 60 years old, we only have 5% of stem cells left only in our organs, thus we need to inject stem cells to rejuvenate ourselves!

I highly recommend the books jointly written by Prof. Dato’ Sri Dr. Mike Chan & Prof. Dr. Dmytro Klokol on biologic medicine especially regarding the knowhow on stem cell technology. These books are well written by using a simple language and presented in a structured as well as coherent manners that will enable both experts and laymen to derive immense benefits from reading these books.

Prof. Dato’ Dr. Chin Yew Sin, JP

Visiting Professor of Zhongshan University, Guangzhou, China Advisor For Pacific Region of Global One Belt One Road Association Deputy President cum Chief Coordinator of Asia-Pacific Region of MHSSATJ, United Nations

Academician Fan Daiming

Stem cells in regenerative medicine is an interesting read consisting of compilation of chapters on the latest advances in stem cell research by international experts in the field. Both authors Prof. Mike Chan and Prof. Dr. Dmitry Klokol are well-known experts in the field of cell therapy and stem cell research. The editor-in-chief, Dr. Dmitry Klokol is a surgeon with extensive experience in surgical practice, who turned into regenerative medicine. Dr Klokol pioneered in creating novice therapeutic protocols of treatment for a number of chronic degenerative diseases. Prof. Mike Chan is a respected expert in cellular therapy and stem cell transplantation product formulation, innovator in cell therapy, and a technical expert in cell therapy manufacturing. Prof. Mike Chan is a Founder of the companies conducting stem cell research, formulation and manufacturing of products for cell therapy and regenerative medicine, as well as medical service in numerous facilities all over the World.

Several chapters are of great relevance to researchers in the field of treatment of chronic degenerative diseases

The entire team of contributing authors have been actively involved in stem cell research for anti-aging, preventive and regenerative medicine. Several chapters are of great relevance to researchers in the field of treatment of chronic degenerative diseases, which otherwise considered as incurable from the point of view of conventional medicine. Some provide a comprehensive overview to new researchers in the field. Others provide extensive details of the protocols related to stem cells procurement, processing, and application. Both medical practitioners and researchers in cell biology and regenerative medicine will find content that interests them.

The book contains many elegant and descriptive images and figures. The tables are generally easy to understand. The book recommended for a broad spectrum of medical-related audience. Wonder, entertaining and enriching!

Academician Fan Daiming

Fan Daiming, from Chongqing city, male, Han nationality, member of the Communist Party of China. Born in November 1953, he joined the Chinese People’s Liberation Army in December 1972. Doctoral tutor, academician of Chinese Academy of Engineering, foreign academician of American Academy of Medical Sciences. Professor of Gastroenterology, Director of the Institute of Digestive Diseases, Director of the State Key Laboratory of Cancer Biology, Director of the National Clinical Pharmacology Base. Former president of the Fourth

Military Medical University. Professional and technical minor rank.

•On June 23, 2017, Fan Daiming won the outstanding President of the

year 2016 Chinese People of Science.

•In 1978, he graduated from the Third Military Medical University.

•In 1981 and 1989, he obtained master’s and doctoral degrees respectively at the Fourth Military Medical University.

•In 1985 and 1991, he went to the National Cancer Center in Tokyo, Japan, and the University of Leuven, Belgium.

•In 2001, he was elected as an academician of the Chinese Academy of Engineering.

•In 2008, he was elected as the 11th National People’s Congress.

•On June 11, 2010, he was elected as the vice president of the Chinese Academy of Engineering.

•On June 29, 2013, he was appointed as an honorary professor at Nankai University. [1]

•On October 21, 2013, he was elected a foreign academician of the Institute of Medicine of the National Academies.

Prof. Yin Dakui

Recent advances in the fields of medicine and technology have led to the development of stem cell therapy. A stem cell is a cell that has the potential to develop into many different types of cell in the body. It has the ability to divide and copy itself and at least one other specialized type of cell. Precursor stem cells are defined on their differentiation pathway.

Stem cells in particular, Progenitor (Precursor) Stem Cells was revealed in such an exhaustive manner here.

Stem Cells in Regenerative medicine: Carpe Diem, Carpe Vitum was written to provide information about the development of stem cell therapy, which can be used in the fields of research and medicine. The main goal of the book is to provide readers with an overview of the scientific facts about precursor stem cells and its promising effects on the human body, as well as on the creation of new drugs and medicines. The book also highlights the ongoing clinical research into stem cells and lists the therapies whose effectiveness is being investigated. Stem cells in particular, Progenitor (Precursor) Stem Cells was revealed in such an exhaustive manner here.

Prof. Yin Dakui

Yin Dakui, male, Hubei native, born in October 1940, graduated from Tongji Medical University. He is currently a member of the National Committee of the Chinese People’s Political Consultative Conference, the chief expert of health education of the Ministry of Health, the president of the Chinese Medical Doctor Association, the president of the China Health Education Association, and the vice chairman and secretary general of the China Health Express Foundation.

•1959 - In 1964, he studied at Tongji Medical University (now Huazhong University of Science and Technology Tongji Medical College).

•In 1964, he was assigned to the First Affiliated Hospital of Huaxi Medical University.

•In 1989, he was promoted to chief physician and professor. He has served as resident physician, attending physician (instructor), deputy chief physician (associate professor) and chief physician (professor) of Huaxi Medical University.

•In 1984, he served as vice president of Huaxi Medical University.

•In 1987, he served as the director of the Sichuan Provincial Health Department.

•In March 1993, he served as Deputy Minister of the Ministry of Health of the People’s Republic of China

Prof. Yu Xiaodong

All chapters are providing novel insights based on new experimental and clinical data.

Stem Cell in Regenerative medicine: Carpe Diem, Carpe Vitum covers contemporary and emerging areas in precursor stem cells original scientific data reports, protocols, novel hypotheses and recommendations for applications. The scope related to stem cells is broad and includes topics in stem cell biology covering basic, clinical, biotechnology, regulatory, and ethical aspects of stem cells and fetal precursor stem cells particularly, as well as adult stem cells, induced pluripotent stem cells, and diseases where stem cell anomalies play a key role and related matters. All chapters are providing novel insights based on new experimental and clinical data. Literature data, scientific research review and hypotheses are providing syntheses of current concepts in the field and discuss emerging issues and controversies around stem cells.