Everand Logo

Welcome to Everand!

  • Discover millions of ebooks, audiobooks, and so much more with a free trial

    Only $11.99/month after trial. Cancel anytime.

    Unavailable
    Fed-Batch Fermentation: A Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli
    Unavailable
    Fed-Batch Fermentation: A Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli
    Unavailable
    Fed-Batch Fermentation: A Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli
    Ebook231 pages1 hour

    Fed-Batch Fermentation: A Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli

    By G G Moulton

    ()

    Currently unavailable

    Currently unavailable

    About this ebook

    Fed-batch Fermentation is primarily a practical guide for recombinant protein production in E. coli using a Fed-batch Fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation process can be accomplished within a single day with minimal supervision. This process can also be done on a small (2L) scale that is scalable to 30L or more. All reagents (media, carbon source, plasmid vector and host cell) used are widely available and are relatively inexpensive. This method has been used to produce three different protein products following cGMP guidelines for Phase I clinical studies.
    • This process can be used as a teaching tool for the inexperienced fermentation student or researcher in the fields of bioprocessing and bioreactors. It is an important segue from E. coli shake flask cultures to bioreactor
    • The fed-batch fermentation is designed to be accomplished in a single day with the preparation work being done on the day prior
    • The fed-batch fermentation described in this book is a robust process and can be easily scaled for CMO production of protein product
    LanguageEnglish
    PublisherElsevier Science
    Release dateOct 16, 2014
    ISBN9781908818331
    Unavailable
    Fed-Batch Fermentation: A Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli
    Author

    G G Moulton

    Garner G. Moulton has a Masters of Science in Biology from the Program of Cell and Molecular Biology at San Diego State University. He has been working in biotech and non-profit research for over 25 years, and has focussed specifically on protein purification and fermentation process development over the last 17 years. During the past 4 years he has been employed by the Infectious Disease Research Institute (IDRI) in Seattle, WA, USA, where he is a principal research associate in charge of the Process Development Department. He has developed many robust fermentation processes that can be adapted to many different recombinant products without having to re-optimize conditions of growth and expression. Numerous projects have been successfully transferred to a contract manufacturer for scale-up production and use in clinical trials.

    Related to Fed-Batch Fermentation

    Related ebooks

    Biology For You

    View More
    View More

    Related podcast episodes

    Related articles

    • Article

      Team Discovers How Bacterium Runs on Methanol From Leaves

      Aug 29, 2017

      New research identifies the genes that a bacterium needs to live on methanol. The work could offer chemists clues to making small carbon molecules into larger ones–something bacteria learned long ago. “Plant leaves are a natural source of methanol, w

      2 min read

    • Article

      Bacteria Could Replace Fossil Fuels For Making Valuable Chemicals

      Apr 26, 2024

      Researchers have engineered bacteria in the laboratory to efficiently use methanol. The metabolism of these bacteria can now be tapped into to produce valuable products currently made by the chemical industry from fossil fuels. To produce various che

      3 min read

    • Article

      Welcome to the Biological Age

      May 1, 2022

      Welcome to the Biological Age
      9 min read

    • Article

      Root Propagation and Powdery Mildew

      Mar 10, 2023

      What are the effects of antimicrobial solutions on cannabis cultivation and production? The cannabis industry is growing at an explosive rate and the regulations governing the cultivation, processing, and distribution are rapidly evolving. They’re al

      4 min read

    • Article

      Bacteria’s Taste For Antibiotics May Clean Up Soil

      May 6, 2018

      New research illuminates key steps in the process of how bacteria turn antibiotics into food. The finding could lead to new ways to eliminate antibiotics from land and water, the researchers say. “It’s just carbon, and wherever there’s carbon, somebo

      3 min read

    • Article

      Plants Grow Brightly With Mushrooms

      Jun 3, 2020

      It’s feasible to create plants that produce sustainable luminescence that we can see, scientists suggest. The bioluminescence found in some mushrooms is metabolically similar to the natural processes common among plants, they say, and by inserting DN

      1 min read

    • Article

      Getting The Terminology Straight

      Sep 24, 2023

      Getting The Terminology Straight
      3 min read

    • Article

      Corn’s ‘Recycling’ System Isn’t Just For Stressful Times

      Dec 27, 2018

      A plant relies on cellular machinery to recycle materials during times of stress, but that same machinery has a remarkable influence on the plant’s metabolism even under healthy growing conditions, according to new research. Autophagy is a process th

      2 min read

    • Article

      ‘Toolbox’ Creates Custom Phages For Killing Pathogens

      Feb 16, 2018

      A new technology platform lets scientists systematically modify and customize bacteriophages, viruses that can attack and kill specific bacteria. These “phages” occur everywhere in the natural world. Precisely because they are matched to just one spe

      3 min read

    • Article

      How Cellular Pumps Protect The Gut From Toxic Stuff

      Oct 9, 2018

      The master regulators of gut stem cells, called intestinal myofibroblasts, have pumps that protect them, and therefore the gut, from the toxic effects of a wide range of compounds, including the anticancer drug tamoxifen, according to new research. “

      2 min read

    • Article

      Opinion: Bringing Order To The ‘Wild Frontier’ Of Microbiome Medicine

      Oct 2, 2018

      When it comes to #microbiome medicine, @US_FDA and @NIH need to create a solid, science-based regulatory framework to help realize the promise of living drugs.

      4 min read

    • Article

      Accident Leads To Basis For New Antibiotics To Kill Resistant Bacteria

      Feb 22, 2024

      Researchers have developed molecules for a new class of antibiotics that can overcome drug resistant bacteria. About a decade ago, researchers in University of California, Santa Barbara chemistry professor Guillermo Bazan’s lab began to observe a rec

      5 min read

    Related categories

    Reviews for Fed-Batch Fermentation

    0 ratings

    0 ratings0 reviews

    What did you think?

    Tap to rate

    Review must be at least 10 words