Science behind Non-specific Science: (For Molecular Biologist & Biotechnologist)
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Science behind Non-specific Science - Vikash Bhardwaj
Glossary
Chapter 1
Complementary: What It Means Broadly?
Introduction
In early history of modern science, proteins were considered most key target molecules to study how cell function. Much of research was focused on characterizing various protein components found in a cell. In 1868/69 a young Swiss physician and scientist Freidrich Miescher working in the laboratory of distinguished biochemist Felix Hoppe-Seyler (discoverer of hemoglobin) at University of Tubingen in Germany, planned to isolate and characterize protein components of leukocytes (white blood cells). From a local surgical clinic, Miescher used to take fresh pus-coated patient’s bandages. Once he received the bandages, he filtered out leukocytes, and used to extract and identify various biological components within white blood cells nuclei using various acidic and alcoholic solutions. He was surprised when he came across a novel substance from cell nuclei that had chemical properties unlike any protein. He found that new class of cellular substance which he has discovered consists of nitrogen, a much higher phosphorous content without sulphur and is resistant to proteolysis. Miescher coined it the term Nuclein
. Although Miescher did most of his work in 1869, his paper on Nuclein
wasn’t published until 1871. Nuclein
was such a unique molecule that Hoppe-Seyler was skeptical about these findings. After a year, Hoppe-Seyler was convinced with Miescher’s results and published those in a journal edited by himself. So Mischer is being credited person who first time isolated nuclei and discovered DNA, a biological molecule which is now well considered as a key to some of the greatest mysteries of life. Later, Miescher also proposed many ideas about biological functions of this molecule. As stated by R. Dahm, Miescher was a brilliant scientist well on his way to making one of the most fundamental discoveries in the history of science, who ultimately fell short of his potential because he clung to established theories and failed to follow through with the interpretation of his findings in a new light