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I. BATERIA: Streptococcus pyogenes H. influenzae type b C. diphteriae Streptococcus pneumonae Bordetella pertussiss Fusobacteria& Borrelia
VIRUSES
Rhinoviruses Coronaviruses
Adenoviruses
DISEASES Common cold Common cold Acute febrile pharyngitis & Pharygoconjuctival fever
Streptococcus pneumoniae
Gram
Culture
Gram
Streptococcus pneumoniae
Optochin
sensitivity test sensitive. Bile solubility test positive . Inulin fermentation positive. Viulence in mice positive.
Streptococcus pneumoniae
sensitive.
Streptococcus pnuemoniae
Optochin
sensitive
Typing of Pneumococci
Capsular
swelling test: 83 serotypes . The type of pnuemococcus is determined by its reaction with type specific sera .
Questions:
What
are the diseases produced by Pneumococcus? How can you diffrentiate betweem Pneumococci & Strept. Viridans?
Corynebacteria
Pathogenic C.diphtheriae
Toxigenic
Non-Toxigenic
Throat swab Gram stained smear It should be differentiated from other causes of pseudomembrane on the tonsils: a) Diphteria : Gram +ve bacilli ,clubbing, intracellular granules, chinese letter writings ve results are common
Culture:
A)
Loefflers serum slope: Enriched medium to demostrate morphology of C.diphterae .3 parts serum+ one part glucose broth Sterilized by INSIPISSATION (70-80 C) for 2 hrs for 3 successive days
Mc
leods tellurite plate: A selective medium for C.diphterae - Bood agar + potassium tellurite - Inhibitory substance: Potassium tellurite As it inhibits other org. allowing only growth of C. diphterae, appears as BLACK colonies - (reduces tellurite to black tellurium)
Blood
slope:
serum
Typical morphology
Mc
BLACK colonies
Blood
agar plate: Beta haemolytic Strept. if the case is: - acute tonsillitis or - Combinecinfection ( diphteria & tosillitis)
Toxigenicity test
A)
Elecks test:
Specimen :nose and throat Swabs Pottasium tellurite Loefflers serum slope Gram stained film &
Toxigenicity tests