ANALYTICAL METHOD DEVELOPMENT AND Click to edit Master SIMULTANEOUS VALIDATION OF subtitle style ESTIMATION OF PANTOPRAZOLE AND ITOPRIDE

BY RP-HPLC
SUBMITTED BY: Anil yadav .N 10021d2312
3/27/12

UNDER THE GUIDENCE OF Prof. Mangamma M.pharm, [Ph.D]

RICHER HEALTHCARE; Kukatpally

Project coordinater:

Chandra sekhar reddy

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CONTENTS

INTRODUCTION AND OBJECTIVE OF WORK PROFILE CONDITIONS

AIM

PLAN

DRUG

EXPERIMENTAL

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INTRODUCTION
HIGH-PERFORMANCE CHROMATOGRAPHY

LIQUID (HPLC)

It is a separating technique which separates mixture of components in to individual components through equilibrium distribution between mobile and stationary phase It is of two types 1.Normal Phase
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2.Reverse Phase

HPLC is of two types Normal Phase Mobile Phase : Polar Stationary Phase : Non - Polar

Reverse Phase Mobile Phase : Non-Polar Stationary Phase : Polar

Separation in HPLC depends up on polarity of the molecule

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AIM AND OBJECTIVE: The main aim of study is to develop simple, accurate method & to improve the conditions & parameters for the simultaneous estimation of Pantoprazole and Itopride by RP-HPLC in Pharmaceutical dosage forms Plan of Work: 1. To collect the drug information available. 2. Obtain physico-chemical properties. 3. Solubility parameters.
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Drug profile :
PANTOPRAZOLE SODIUM DRUG CATEGORY OR Benzimidazole CLASS ITOPRIDE HYDROCHLORIDE

MOL.WT CHEMICAL FORMULA SOLUBILITY

proton pump inhibitor 383.4 g/mol C16H15F2N3O4S Freely soluble in ethanol, soluble in Water & Slightly soluble in Hexane 3.92

Gastro prokinetic agent 394.9 g/mol C20H26N2O4,HCL Freely soluble in water, methanol and sparingly soluble in acetic acid 9.47951

PKa Structure

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Literature review of Pantoprazole and Itopride

COLUMN MOBILE PHASE

Phenomenex (100 C18, 4.6 mm x 3.5) Buffer: phosphate buffer and acetonitrile in the proportion of 55:45 (v/v) Buffer pH=5.0 1 ml/min Ambient 289 nm

pH FLOW RATE TEMPERATURE DETECTION

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