Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
cbaracterIstIcs oI a maIe's
semen and tbe sperm
contaIned In tbe
semen.
W measures tbe amount oI
semen a man produces.
W determInes tbe number and
quaIIty oI sperm In tbe
semen sampIe.
baracterIstIcs
MorpboIogy
Number
VoIume
MotIIIty
bemIcaI
$emen
W aIso caIIed sem1no1 ]1u1d presen1 1n
mo1es
W medIum tbe sperm can swIm
W contaIns spermatozoa
W a vIscId mucIIagInous IIuId wItb IaInt
yeIIow coIor
W Has cbaracterIstIc odor caIIed semInaI
odor.
$emen consIst oI tbe IoIIowIng
1. $permatozoa (10%)
2. $emInaI PIasma (90%)
3. EpItbeIIaI eII (< 1%)
W $perm
-reIers to tbe
maIe reproductIve
ceIIs.
Purpose oI $emen AnaIysIs
1. To verIIy tbe success oI vasectomy
(usuaIIy done 6 weeks aIter tbe
vasectomy)
Vasectomy- a surgIcaI procedure In
wbIcb tbe vasa deIerentIa oI a man
are severed, and sometImes tIed or
seaIed. TbIs resuIts to sterIIIty.
2. to InvestIgate InIertIIIty
PrerequIsItes Ior semen
coIIectIon
W AvoId Iong perIod oI sexuaI
InactIvIty.
W AvoId any sexuaI actIvIty tbat
resuIts In ejacuIatIon Ior 2 to S
days beIore semen coIIectIon.
W Tbe subject sbouId be provIded wItb
cIearIy wrItten or oraI InstructIons
concernIng tbe coIIectIon and
transport oI tbe semen sampIe.
W Tbe name oI tbe person, perIod oI
abstInence, date and tIme oI
coIIectIon, and any medIcInes or
berbaI suppIements tbat tbe person
Is takIng sbouId be recorded.
$ampIe coIIectIon and
deIIvery
1. Iean tbe bands and penIs
properIy.
2. Tbe sampIe sbouId be obtaIned by
masturbatIon.
ays oI coIIectIon
W MasturbatIon
- a sexuaI stImuIatIon (not
Intercourse) tbat produces an orgasm.
W $urgIcaI Procedure
W DurIng Intercourse usIng a non-
spermIcIdaI condom
3 types oI surgIcaI
procedures
MIcrosurgIcaI EpIdIdymaI
$perm AspIratIon (ME$A)
-used In patIents wItb
reproductIve tract bIockage.
TestIcuIar $perm
AspIratIon (TE$A)
- tbe testIcuIar tIssue Is sucked
out tbrougb a IIne needIe, under
IocaI anaestbesIa.
Percutaneous EpIdIdymaI $perm
AspIratIon (PE$A)
tbe sperm Is sucked out Irom tbe
epIdIdymIs by puncturIng It wItb a
IIne needIe.
W 411us 1n1errup1us aIso
known as wItbdrawaI or tbe
puII-out metbod. It may be
used aIter a vasectomy to test
Ior tbe presence oI sperm, but
It Is not acceptabIe as a means
oI coIIectIon because oI tbe
IoIIowIng:
W tbe IIrst portIon oI tbe ejacuIate,
wbIcb contaIns tbe bIgbest
concentratIon oI spermatozoa, may
be Iost
W tbere wIII be ceIIuIar and
bacterIoIogIcaI contamInatIon oI
tbe sampIe
W tbe acId pH oI tbe vagInaI IIuId wIII
adverseIy aIIect sperm motIIIty
W
3. Tbe sperm Is ejacuIated Into a
cIean, wIde-moutbed gIass or
pIastIc contaIner, or a non-
spermIcIdaI condom.
ontaIner Ior $emen sampIe
W Iean, wIde-moutbed gIass
W Iean, wIde-moutbed pIastIc (no
toxIc eIIect on spermatozoa)
W Non-spermIcIdaI condom (not just
tbe ordInary condoms)
*contaIners sbouId be warm to
mInImIze tbe rIsk oI coId sbock
. It Is recommended to get tbe IIrst
part oI tbe ejacuIatIon as It Is
tbougbt to be tbe most sperm-rIcb.
S. Two semen sampIes sbouId be
coIIected Ior InItIaI evaIuatIon.
AddItIonaI semen sampIes may be
needed.
6. Protect tbe sampIe Irom extremes
oI temperature (not Iess tban 20c
and not more tban 0c) durIng
transport to tbe Iaboratory.
?. Tbe sampIe sbouId be examIned
ImmedIateIy aIter IIqueIactIon and
wItbIn 1b oI ejacuIatIon
#uIes In semen coIIectIon and
deIIvery
W Tbe sampIe sbouId be coIIected
aIter a mInImum oI bours and no
Ionger tban ? days oI sexuaI
abstInence.
* DuratIon oI abstInence
W more tban ? day: sperm motIIIty
may decIIne
W <b: sperm concentratIon may
decIIne, but tbe same motIIIty
W Tbe tIme IntervaI between tbe Iast
ejacuIatIon and sampIe coIIectIon
sbouId be weII deIIned and
preIerentIaIIy as constant as possIbIe.
W Tbe name oI tbe person, perIod oI
abstInence, date and tIme oI coIIectIon
sbouId be recorded.
W Any medIcInes or berbaI
suppIements tbat tbe person Is
takIng sbouId be noted
W Two semen sampIes sbouId be
coIIected Ior InItIaI evaIuatIon.
W Tbe coIIected sampIes sbouId not
be Iess tban ? days or more tban 3
montbs apart.
W Tbe bands and penIs sbouId be
cIean wben masturbatIng.
W Tbe sampIe must be deIIvered to
tbe Iaboratory wItbIn 1 bour aIter
coIIectIon.
W $emen oIder tban 1 bour sbouId not
be accepted Ior .rg4preservo114n
(rapId IreezIng In IIquId or vapor
nItrogen at -196 to preserve
sperm Ior Iuture use).
W Tbe sampIe sbouId be stored In
temperature not Iess tban 20c and
not more tban 0c durIng
transport to tbe Iaboratory.
W Do not reIrIgerate tbe sampIes.
W HandIe tbe sampIe wItb care.
&nacceptabIe $pecImens
W a. contaIner Is cracked, broken or
IeakIng
W b. contaIner not approved by tbe
Iaboratory
W c. contaIner Is bot or coId to tbe
toucb
W d. specImen coIIected by use oI
condom not approved by tbe
Iaboratory
W e. specImen brougbt In by
unscbeduIed waIk-In appoIntments.
W I. IncompIete sampIes, wbereIn tbe
IIrst portIon oI tbe ejacuIate Is Iost.
$emen ExamInatIon
W Tbe Parameters In TestIng can be
cIassIIIed In two categorIes:
MacroscopIc ExamInatIon and
bemIcaI ExamInatIon
MA#$PI
EXAMINATIN
W $perm ount
W VoIume
W MorpboIogy
W MotIIIty
W oIor
W VIscosIty
W LIqueIIcatIon tIme
TotaI sperm count
W %41o1 sperm .4un1, or 141o1 sperm
number, Is tbe totaI number oI
spermatozoa In tbe entIre ejacuIate.
By H, Iower reIerence IImIt Is 39
mIIIIon per ejacuIate
W Average sperm concentratIon Is
more tban 60 mIIIIon per mIIIIIIter
(>60 mIIIIonJcc). ounts oI Iess
tban 20 mIIIIon per mIIIIIIter (<20
mIIIIonJcc) are consIdered sub-
IertIIe.
VoIume
NormaI ejacuIated voIume Is between 2
and 6 mI.
H regards 1.S mI as tbe Iower
reIerence IImIt
Low voIume may IndIcate partIaI or
compIete bIockage oI tbe semInaI
vesIcIes or tbat tbe man was born
wItbout semInaI vesIcIes.
MorpboIogy
W IassIIIcatIons:
NormaI ovaI-sbaped
Tapered
Amorpbous
DupIIcated
Immature
NormaI spermatozoId
vaI Iorm wItb smootb contour
AcrosomaI cap encompassIng 0-?0%
oI bead
No abnormaIItIes oI mIdpIece or taII
No cytopIasmIc vacuoIes oI more tban
baII oI tbe sperm bead.
Head sIze oI S-6 M x 2.S-3.S M
$trIct crIterIa Ior normaI
sperm morpboIogy:
W $perm bead
$mootb ovaI conIIguratIon
Lengtb: S-6 mIcrons
Idtb: 2.S-3.S mIcrons
Acrosome comprIses 0-?0% oI tbe
anterIor sperm bead.
W MIdpIece
AxIaIIy attacbed
, 1.S tImes tbe bead Iengtb
Iess tban or equaI to 1 m In wIdtb
W TaII:
$traIgbt
&nIIorm
sIIgbtIy tbInner tban tbe mIdpIece
uncoIIed
S m Iong
$perm morpboIogy
$taInIng metbods
Tbe PapanIcoIaou staIn and tbe
$borr staIn are tbe metbod most
wIdeIy used
Tbe DIII-QuIk rapId staInIng metbod
Is used too
W It may bave background staInIng
and may not aIways gIve tbe same
quaIIty as tbe PapanIcoIaou staIn or
$borr staIn.
$perm morpboIogy
PIoios and scIcmaiic drawings of somc ,-3472,
14728of Iuman scrmaiozoa.
NormaI sperm bead