histology of Tooth Development

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Stages of Tooth Development

Dental lamina Bud Cap Bell Appositional Repeated by Successional lamina
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Early Dental Lamina

Tongue

Dental lamina Vestibular lamina

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rxdentistry.net

Bud Stage

Cap Stage of Tooth Development

Dental (enamel) organ Dental papilla Dental follicle

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Copyright 2007, Thomas G. Hollinger, Gainesville, Fl

Cap Stage

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Copyright 2007, Thomas G. Hollinger, Gainesville, Fl

Bell Stage

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Oral Histology, 5th edition, A R Ten Cate

Copyright 2007, Thomas G. Hollinger, Gainesville, Fl

Oral cavity Outer dental epithelium

Dental lamina
Enamel knob

Stellate reticulum
Stratum intermedium

Inner dental Epithelium

Dental papilla

Structures Seen at Bell Stage

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Cervical Loop
Acellular zone Preameloblasts (IDE or IEE)

Stratum intermedium
Stellate reticulum

ODE (OEE)

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Cervical loop
Acellular zone

Dental follicle Dental organ

Structures of the Dental Organ

Dental papilla

ODE
Stellate reticulum Stratum intermedium

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Appositional Stage

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Oral Histology, 5th edition, A R Ten Cate

Dental follicle ODE Stellate reticulum IDE Pulp Odontoblasts Stratum intermedium

Structures at Appositional Stage

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Dentinogenesis

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Oral Histology, 5th edition, A R Ten Cate

Details of Tooth Development

Cell free zone
Odontoblasts

Ameloblasts
Preameloblasts

Preodontoblasts
Acellular zone
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Ameloblast with Tomes process

Oral Histology, 5th edition, A R Ten Cate

Incorporation of Tetracycline during Dentinogenesis

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Some Types of Dentin

Mantle dentin

Tertiary dentin
Primary dentin

Secondary dentin
Predentin

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Oral Histology, 5th edition, A R Ten Cate

Amelogenesis
Enamel proteins
Amelogenins (Bulk of proteins) Tuftelin (Just at the DEJ) Enamelin 2% Amelins/Ameloblastins 5-10%

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Early Apposition

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Distal End of a Ameloblast

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Distal End of a Ameloblast

Forms rod sheath

Forms enamel rod Forms interrod substance

Proximal portion of process

Junctional complex

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Tomes Process

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Enamel

Secretory Ameloblast

Tomes process

Junctional complex

Cell body of ameloblast

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Oral Histology, 5th edition, A R Ten Cate

Grazing Section through Forming Enamel

Interrod substance forms first to establish a mold for the enamel rod

Terminal bar (in TEM one sees the junctional complex)

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Developing Enamel

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Root Formation

Cervical loop forms the epithelial root sheath (of Hertwig)

Cervical loop Dental papilla

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Root Formation

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Cells of the dental follicle (forms cementocytes)

Cell rest (of Malassez)

PDL, Cell Rests, and Cementum

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Granular Layer of Tomes

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Cellular Cementum
Acellular cementum
Cellular cementum

Hyaline layer (of Hopewell Smith)

Granular layer of Tomes Dentin (with tubules)

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Junctional Epithelium
Basement membrane shown in green
B = Alveolar mucosa C = Sulcular epithelium D = Basement membrane of junctional epithelium (derived from the reduced enamel epithelium)

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Thanx. !!
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