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• Free of vibration
• No large temperature
fluctuations
• No direct sunlight and
• No drafts
Weigh Balance Calibration
• Calibration is important to
ensure that the instrument
accurately weighs
substances for reagent
preparation
• Test results may be
incorrect due to instrument
malfunction
Weigh Balance Calibration
(continued)
• Use NIST Class S weights for
calibration
• Verify that class S weights and balance
pan are free from dust and fingerprints
• Only “User” calibration will be
discussed here
• Note: NEVER touch the weights or the
balance pan with bare hands!
“User” Calibration Method
• The “User” method is used to calibrate
the balance using a mass of known
value
• Three readings should be taken for the
six specified weights: 50, 100, 200,
500, 2000 and 4000
• Record value in grams
“User” Calibration Method
(continued)
– Document each
Laboratory: ___________________
Balance: ______________________
Technician: ____________________
1 mg 10 mg 50 mg
information:
Date Tech 10 mg 10 mg + 1 mg 50 mg 50 mg + 1 mg 100 g 100 g + 1 g