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GENUS:
CAMPYLOBACTER
Prof. Khalifa Sifaw Ghenghesh
• Slender, Helically Curved
Rods.
• Corkscrew-Darting Motility.
• Oxidase: +ve
• Microaerophilic >
–Campy. jejuni
–Campy. coli
–Campy. lari (Campy. laridis)
–Campy. Fetus
• Arcobacter >> A. butzleri
Campylobacter fetus
Leifson flagella stain
Campylobacter jejuni
Campylobacter jejuni
Campylobacter jejuni
thin, comma-, S-, or gull-winged shaped forms
(48 h culture)
VIRULENCE FACTORS
• Motility
• Adherence
• Invasion
• Toxin Production
–Cytotoxins:
–Enterotoxins
DISEASE
• in Animal:
• in Man:
– Diarroeal Illness:
– Guillain-Barre Syndrome (GBS):
LABORATORY DIAGNOSIS
• Specimen:
• Culture:
– Selective media >> Skirrow's >
• Incubation:
– Temp.
– microaerobic gas generation packs
• Identification:
– Oxidase, Gram stain, Hippurate hydrolysis
Blood agar plate culture of
Campylobacter fetus s. intestinalis
TREATMENT
• Campylobacter enteritis
– Self-limiting
– Fluid and electrolyte replacement
• Erythromycin
• Fluoroquinolones
– Ciprofloxacin
EPIDEMIOLOGY
• Incidence
• Source of Transmission
– Poultry and Raw Meats
– Other routes of Infection
CONTROL
• Education:
– Keep food that will be eaten raw, such as vegetables, from
becoming contaminated by raw animal-derived food products.
– Thoroughly cook all food products from animals, especially
poultry, and avoid consuming unpasteurized milk, or other
unpasteurized products.
– Cases should not prepare food for other individuals or attend
child care until diarrhea has resolved.
– Educate case and household contacts on proper hand washing
techniques.
– Always wash hands thoroughly with soap and water before
eating or preparing food, after using the toilet, after changing
diapers, and after touching pets or other animals (especially
puppies and kittens with diarrhea).
– After changing diapers, wash your hands AND the child’s
hands.
– In a childcare setting, dispose of stool and soiled diapers in a
sanitary manner.
CONTROL
• Food Handlers:
GENUS:
HELICOBACTER
Prof. Khalifa Sifaw Ghenghesh
• Spiral, curved/straight G-ve
rods.
• Motile.
• Microaerophilic / 37oC.
• Oxidase +ve
• > 15 species of Helicobater
–H. pylori
Helicobacter pylori
3D morphology
Helicobacter pylori
showing typical thin, comma- or S-shaped
forms (72 h culture).
1. H. pylori
• Natural Habitat:
- Human gastric mucosa.
- Dental plaque.
- Human faeces.
• Transmission:
- Faecal-oral or Oral-oral route.
- Flies ???
• Virulence Factors:
–Urease, Phospholipase A, etc.
• Clinical Significance:
–Duodenal Ulcer & Gastritis.
–Gastric adenocarcinoma.
LABORATORY DIAGNOSIS
1. Invasive Tests:
– Specimen: Gastric mucosa.
– Microscopy:
– Culture: Selective media
– Biopsy urease test:
2. Non-Invasive Tests:
– Serology: Abs to HP
– Urease breath test:
TREATMENT
Twice-Daily PPI-Based Triple Therapies
• Cure rate 90%: 2 weeks (? 10 days) in U.S., 1 week
outside U.S.
• Omeprazole 20 mg bid or Lansoprazole 30 mg bid
• Clarithromycin 500 mg bid
• Amoxicillin 1 g bid or Metronidazole 500 mg bid
• Clinical significance:
– Proctocolitis, Proctitis, Enteritis,
Bacteraemia.