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Sujeewa S. Palayangoda
• strategy: flow the mixture over a material that retains some components more than
other different components flow over the material at different speeds.
• Column chromatography
• Paper chromatography
• Thin-layer chromatography
• Gas chromatography
• Ion-exchange chromatography
chromatography, for which they were presented the Nobel Prize in chemistry
in 1952.
Gas Chromatography is a versatile instrument in chemistry
RESET
Regulators Syringe/Sampler
Inlets
Detectors
Argon
Hydrogen
Oxygen
Column
Basic components of Gas Chromatograph
• Gas
• Flow control
• Injector
• Oven, Column
• Detector
• Eluents
• Recorder
• Gas
• Flow control
Cross-section of a column
These factors affect the separation
• Intermolecular interactions
1. Orientation interactions
The interactions between two permanent dipoles
2. Debye interactions
The interactions between a permanent dipole in one molecule
and the induced dipole in a neighboring molecule
3. Dispersion interactions
The forces arising out of synchronized variations in the
instantaneous dipole of the two interaction species
Commercially available GC detectors and their applications
Detector Application
Flame Ionization (FID) Carbon compounds
Thermal Conductivity Universal
(TCD)
Mass spectrometer (MSD) Variety of compounds
FID TCD
•Each component of the mixture reaches the detector at a different time and
Starting Point
On Chart
Distance
Peak Area by the
Triangulation Method
Peak Area
Triangulation Method
Peak Area = h * w½
w½ = width of peak at
½ the Peak Height
Total Peak Area(TA) = A + B
Mole Fraction(MF) = A/TA, B/TA
Mole Percent = MF x 100
Recording
The detector information is sent to the printer that produces hard copy
of the chromatographic run.
• The area under each peak correlates with the relative amount of each
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