Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
Presented by:-
Raina J. P. Khanam
2nd year
CONTENTS
• Definition
• Types of immunity
• Cells of immune system
• Complement system
• Transendothelial migration
• Leucocytes function
• Specific immune response
2
Immunity
It is defined as the resistance exhibited by the host against any
foreign antigen including microorganisms.
3
Immune system
The immune system is a network designed for the homeostasis
of large molecules (oligomers) and cells based on specific
recognition processes.
4
5
Types of immunity
1. Innate immunity
2. Adaptive immunity
6
Cells of immunity
Cells of the immune system that are important in inflammation and host defenses include
1. mast cells
2. peripheral dendritic cells
3. neutrophils
4. monocytes/macrophages
5. T cells
6. B cells
7. natural killer cells (NK).
Other hematopoietic leukocytes, including basophils, eosinophils, erythrocytes, and
platelets, also participate in certain forms of inflammation or immune function.
7
8
Innate immunity
Innate immunity or non specific immunity is the natural
10
1. Mast cells
Mast cells are important in immediate inflammation.
More recently, mast cells have been shown to express toll-
like receptors. These receptors allow the innate immune
system to adapt (e.g., express the major histocompatibility
complex (MHC) class II molecules, produce nitric oxide and so
on.
11
Mast cells feature prominent cytoplasmic granules, termed lysosomes,
which store inflammatory mediators such as
1. histamine
2. eosinophil chemotactic factor
3. neutrophil chemotactic factor
4. heparin.
13
Dendritic Cells express high levels of
1. MHC class II molecules
2. intercellular adhesion molecule-1 [ICAM-1]
3. leukocyte function–associated antigen-3 [LFA-3])
4. co-stimulatory factors.
14
3. Neutrophils
Neutrophils, also known as polymorphonuclear
leukocytes (PMNs), are the predominant leukocyte
in blood approx. (4000-8000 cells/mm3).
19
The complement system is a central component of
inflammation that enables endothelium and leukocytes to
recognize and bind foreign substances for which they lack
receptors. Complement promotes inflammation by
generating the following:
1. A Vasoactive substance, termed kinin-like, C2a, which induces pain
and increases vascular permeability and dilation.
2. Molecules, termed anaphylatoxins, C3a and C5a, which produce
anaphylaxis by inducing mast cell secretion.
3. A chemotaxin, C5a, which attracts leukocytes and stimulates
phagocyte secretion.
4. An opsonin, C3b, covalently bound to molecular aggregates, particles,
or cells, which enables phagocytes to ingest them.
20
C3 is the most important component of complement system.
A sequestered, internal thioester bond is the essential feature of C3,
and it shares this feature with the related molecule, C4.
Splitting of C3 forms C3a and C3b and exposes the internal thioester
bond residing within the C3b fragment.
Two main pathways result in the splitting of C3: the alternative and
classical pathways.
Both the alternative pathway and the classical pathway lead to
inflammation and phagocytosis through an enzyme that is designated a
bound C3 convertase.
The alternative pathway leads to the hydrolysis of C3b by larger
structures (designated R), including the hydroxyls and amines on
macromolecules and bacterial cell surfaces. The resulting covalent
structure,
21 C3b-R, leads to the bound C3 convertase (R-C3bBb).
The classical pathway is initiated in response to the presence of some
irritant.
This pathway involves the activation of a serine protease (e.g., C1qrs) that
has attached to an irritant.
These new complexes can bind and cleave both C3 and C5; thus
they are called C3/C5 convertase.
23
The C5 convertase activity is monumentally slow
(one of the slowest enzymatic activities known).
26
Leukocytes use the lectin (a non-enzymatic carbohydrate-binding
protein) designated L-selectin to interact with carbohydrate molecules
known as vascular addressin on the luminal surface of endothelial cells.
28
The interaction of a chemokine, interleukin-8 (IL-8), with the leukocyte
receptor CXCR2 causes the leukocyte to shed L-selectin and upregulate the
integrin leukocyte function–associated antigen-1 (LFA-1).
LFA-1 binds intercellular adhesion molecule-2 (ICAM-2), which is expressed
constitutively by endothelium. This results in rolling arrest because the
phagocyte becomes firmly associated with the endothelium.
CD31 (platelet-endothelial cell adhesion molecule-1) is a transmembrane
glycoprotein present at the intercellular borders of endothelial cells facing into
lumen and on all leukocytes.
Once the leukocyte locates the inter-endothelial junction, it uses its own CD31
as a zipper.
This zipper effect has been proposed as a mechanism of minimizing the leakage
of fluid. As the endothelium unzips its CD31, the leukocyte rapidly “zips”
between the endothelial cells.
Leukocytes possess several proteases, including uro-kinase plasminogen activator
receptor (uPAR). The uPAR leads to the activation of collagenase, which then
degrdae
29
the basement membrane and enable the leukocyte to enter the
connective tissue.
LEUKOCYTE FUNCTIONS
1. Chemotaxis
2. Phagocytosis
3. Antigen processing and presentation
1. Chemotaxis
Once the leukocyte enters the connective tissue, it must be able to locate
and migrate to the site of insult.
31
2. Phagocytosis
Phagocytosis is the process by which cells ingest particles of a size visible
to light microscopy.
The immune system has evolved mechanisms of coating the pathogen with
a few recognizable ligands, which enable the phagocyte to bind and ingest
the pathogen. This is referred to as opsonization.
32
Once a microbe has been ingested, it may be killed. Phagocytes kill bacteria
through two broad categories of killing mechanisms.
33
Specific granules contain microbiocidal components like
Lysozymes
Lactoferrin
Lactoferricin
These are a class of Pattern Recognition Receptor (PRRs) that recognises Microbiota
associated molecular patterns (MAMPs) and that signals cytokine secretion in innate cells.
Among the 10 human toll-like receptors identified so far, toll-like receptor-2 and toll-
like receptor-4 are the most defined members.
Toll-like receptor-2 is mostly involved in the recognition of a variety of different
bacterial cell components, such as peptidoglycans and lipoproteins.
Toll-like receptor-4 has been shown to specifically recognize lipopolysaccharide of Gram-
negative bacteria and acts in cooperation with several protein components, such as
lipopolysaccharide-binding protein and CD14.
The gingiva is constantly exposed to microbes present in plaque biofilm, toll-like receptors
signaling plays an important role in the innate immune response and maintenance of
periodontal
36
health. However, over-production of pro-inflammatory cytokines due to
chronic stimulation of toll-like receptors, they may lead to tissue destruction.
37
TLR signaling pathway……
The Toll-Like Receptors are single-pass transmembrane proteins
characterized by an N-terminal leucine-rich recognition domain and an
intracellular C-terminal Toll/IL-1 receptor signaling domain (TIR).
The TLR signaling pathways highlights the potential for synergy or amplification
effects modulating the host immune response.
40
ANTIGEN PRESENTATION
41
However, binding of T-lymphocytes to MHC/ or CD1 cluster alone does not activate the T-
lymphocytes into action.
So in order to become activated T-cell must receive a second signal from APC.
B –cell also require co-stimulation to proliferate further after initial recognization and binding
to the antigen.
42
Adaptive immunity
The adaptive immune system is also known as the acquired
immune system/specific immune system.
It is activated by the non specific innate immune system.
Lymphocytes (e.g., T cells, B cells) get stimulated through
their B and T cell antigen receptors (BCRs and TCRs)
present on the cell membranes which facilitates the specific
targets.
44
1. T Cells
The high-affinity interaction between BCR and antigen enables the B cell
to bind and ingest the antigen without antigen presentation. Ingested
antigen is degraded and presented to T cells.
47
3. Natural Killer (NK) Cells
• NK cells recognize and kill certain tumor and virally infected cells.
48
SPECIFIC IMMUNE RESPONSES
Following events that take place are:
1. Clonal selection – selection and activation of specific lymphocytes which are
capable of recognizing specific Antigen.
2. Clonal expansion – increase in the number of activated lymphocytes (up to
5000 times).
3. Clonal contraction – apoptosis of activated lymphocytes after completing their
job.
4. Memory – maintenance of these activated lymphocytes in small number till the
time body encounters the same antigen again. These cells will cause rapid
immune response (secondary immune response).
When the body encounters the same antigen again the memory cells
will induce clonal expansion at a rapid phase (1 to 3 days).
49
T-CELL RESPONSES
50
B-CELL RESPONSES AND ANTIBODIES
B cells produce immunoglobulins at the site of infection from local and systemic tissue
sources.
Immunoglobulin accounts for about 20% to 25% (15 mg/ml) of the total serum protein
(60-70 mg/ml).
Humans possess nine genetically distinct isotypes of immunoglobulins: IgM, IgD, IgG1, IgG2,
IgG3, IgG4, IgA1, IgA2, and IgE.
The ability of B cells to respond to antigen depends on the B-cell antigen receptor (BCR).
The51BCR is formed partly by immunoglobulin molecules on the B-cell surface, which serve
as highly specific antigen receptors.
B cells are capable of responding to certain antigens in the absence of T cells.
This is referred to as T-independent B-cell antibody response.
However, in some situations B-cells are activated by the T-cells. This is known
to be T cell dependent antibody response.
In this pathway B cell bind soluble antigens and then they are ingested and
processed. Thus mechanism of binding antibody to antigen results in
protection via:-
1. Neutralization
2. Agglutination
3. Opsonization
4. Complement activation
5. Enhanced NK cells
52
53