EVALUATION OF ANTI ULCERATIVE

COLITIS ACTIVITY OF BARLERIA

BUXIFOLIA IN WISTAR RATS
Project Work Proposal Submission To

Institutional Animal Ethics Committee

P.RAMIREDDY MEMORIAL COLLEGE OF PHARMACY

Under the Guidance of Presented by:

Dr .C. Rajaram K.Yojana
Professor Regd. No.: 15441R0027
Department of pharmacology
 CONTENTS

 INTRODUCTION
 REVIEW LITERATURE
 AIMS AND OBJECTIVES
 PARAMETERS
 PROPOSED TREATMENT SCHEDULE
INTRODUCTION
DEFINITION:
Ulcerative colitis is an inflammatory
bowel disease that causes inflammation and
ulcers in digestive tract. It mainly affects to the
large intestine or colon. It causes irritation and
swelling called inflammation eventually that
leads to the source called ulcers in the lining of
large intestine or colon.
TYPES OF ULCERATIVE COLITIS:
There are several other types of ulcerative
colitis. The following is the description of the
different types of ulcerative colitis:
 Ulcerative proctitis
 Proctosigmoiditis
 Left – sided colitis
 Pan – ulcerative(total) colitis
SIGNS AND SYMPTOMS:
Signs and symptoms may include:
 Diarrhoea, often with blood or pus
 Abdominal pain and cramping
 Rectal pain
 Rectal bleeding
 Weight loss
 Fatigue
 Fever
 In children, failure to grow
RISK FACTORS:
Risk factors may include:
Age
Race or ethnicity
Family history
Environmental factors
CAUSES:
Environmental factors
Immune system
Genetics
High intake of lenoelic acid
COMPLICATIONS:
Possible complications of ulcerative colitis
include:
a. Severe bleeding
b. Severe dehydration
c. A hole in the colon
d. Liver disease
e. Bone loss
f. Inflammation of skin and joints
g. An increased risk of colon cancer
h. A rapidly swelling colon
PATHOPHYSIOLOGY:
 Inflammatory process usually confined to
rectum and sigmoid colon.
 Inflammation leads to mucosal haemorrhages
and abscess formation which leads to necrosis
and slough of bowel mucosa.
 Mucosa becomes red friable and ulcerated
bleeding is common.
 Chronic inflammation leads to narrowing and
shortening of colon.
 TREATMENT
S.N DRUG MECHANISM OF THERAPEUTIC USES ADVERSE EFFECTS
O ACTION

1. Amino salicylates like: Olsalazine, Salfa i. Blocks the i. Treatment of mild i. Headache
salazine production of and moderate ii. Nausea , vomiting
prostaglandins ulcerative colitis iii. Gastric distress
ii. Inhibits the ii. Treatment of iv. Haemolytic
activation of inflammatory anaemia
nuclear factor bowel disease v. cyanosis
(kappa)B

2 Corticosteroids like : prednisone , hydro i. Inhibits leukocyte i. for the treatment of i. Diabetes
cortisone infiltration at the primary and ii. Headache
site of inflammation secondary adreno iii. Hyper tension
ii. Suppress humeral cortical iv. Facial hair growth
immune response insufficiency v. Swelling of legs
controls the ii. Treatment of vi. Puffiness of the face
production of ulcerative colitis
prostaglandins and and liver disorders
leucotrienes

3 Azathioprine i. Inhibits DNA and i. Used during organ 1. Nausea

RNA synthesis and transplantation 2. Vomiting
prevents purine ii. Used to treat 3. Diarrhoea
synthesis rheumatoid arthritis 4. Mild leucopoenia
ii. Blocks lymphocyte iii. Used in the 5. Abnormalities in
proliferation invitro treatment of liver function
and production of vasculitis
interleukins and
shows
antiproliferative
activity
 REVIEW
LITERATURE
PLANT PROFILE:
 Botanical name: “Barleria buxifolia”
 Synonym: Barleria coriacea
 Common name: Box – leaved barleria
 Family : Acanthaceae
 Order :Lamiales
VERNACULAR NAMES:
Telugu : Erramullugoranta
Tamil : Kattimullu
Sanskrit : Iksura
Kannada : karichulli
Malayalam : karicculli
CLAIMED USES :
From Roots and leaves:
 Ulcerative colitis
 Stomach ache
 Tonic
 Febrifuge
 Cough
 Bronchitis
 Inflammation
 Diabetes
CHEMICAL CONSTITUENTS:
 Alkaloids
 Carbohydrates
 Flavanoids
 Flavones
 Saponins
 Phenols
 Acid compounds
EARLIER WORKS:
The earlier works which are done on the plant
“Barleria buxifolia” are:
 Anti oxidant activity.
 Anti microbial and cytotoxic activities.
 Anxiolytic and antidepressant activity.
 Antifeedant activity.
 REPORTED PLANT WITH ANTI
ULCERATIVE COLITIS ACTIVITY:
S.NO PLANT NAME FAMILY ANIMAL MODEL

1. Barleria buxifolia Acanthaceae Wistar rats

2. Eucheuma spinosum solieriaceae Guinea pig

3 Calotropis procera linn. Asclepiadaecae Wistar rats

4 Haplophylum tuberculatum Rutaceae Wistar rats

 AIMS AND OBJECTIVES:
The main aim of the present work is “Evaluation of
anti ulcerative colitis activity of Barleria
buxifolia in Wister rats”.
The main objectives present in the present work is:
Selection of the plant.
Collection and authentication of plant.
Extraction of crude drug.
Healing of effect of crude extract in rat model of
ulcerative colitis.
 PARAMETERS
The parameters to be monitored include the following:
 Colon mucosal damage index (CMDI)
 Disease activity index (DAI)
a) Body weight
b) Stool consistency
c) Faecal occult blood test (FOBT)
 Myeloperoxidase (MPO)
 In vivo anti-oxidant parameters
a) Catalase (CAT)
b) Reduced glutathione (GSH)
c) Malonyldialdehyde (MDA)
Proposed Treatment
Schedule
S. No. GROUPS No. of ANIMALS TREATMENT

1 Normal 6 Vehicle ( Distilled water )

2 Control 6 Acetic acid ( 4%v/v, rectal route )

3 Standard 6 5-ASA (100 mg/kg, p.o )

Acetic acid (4%v/v, rectal route) + Plant

4 Treatment-1 6
Extract (200mg/kg, p.o )

Acetic acid (4%v/v, rectal route) + Plant

5 Treatment-2 6
Extract (400mg/kg, p.o)

No.of Animals required: 30

No.of Animals to be sacrificed: 5