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BIOTC 104

BIOCHEMIST
RY
Laboratory
Grading System
Midterm and Finals - 40%
Laboratory Reports - 25%
Quizzes - 20%
Laboratory Performance/ -
15%
Attendance
Total 100%
Classroom Management
Bring your individual laboratory
gown, gloves, goggles and masks.
Then your own pentel pens,
masking tape for labelling, tissue,
towels, soap for hand washing and
liquid detergent for the cleaning of
glass wares.
Absences and lates
Encouraged to do your own
researches. It’s okey that you have
Laboratory performance will be rated
by your self, peers and by the
teacher.
Cellphones are not allowed.
Don’t sit when doing the experiment
and don’t drag the chairs.
Bags should be placed inside the
drawer. Only laboratory notebooks,
pens can be seen in your table and
glass wares.
 Absences
General Safety Guidelines
Laboratory clothing
Laboratory gown/cloth
Cotton- good against flying
objects, sharp or rough edges.
Wool- protects against molten
splashes, small acid spills and
small flames.
Synthetic fibers- protect against IR
and UV radiation but burns easily
Safety Glasses
Working alone in the laboratory
Unauthorized experiments
Read up experiment procedure
Food
Cleanliness
Waste Disposal
Fume Hood
Burners
Hot objects
Glass rods
Glass ware
Watch-out for:
Chipped tips on burets, pipets, funnels,
graduated cylinders and test tubes
Chipped , cracked and broken rims on
beakers, flasks, test tubes and crucibles
Sharp edges on glass tubings and glass rods
Inflexibility in rubber stoppers and rubber
tubings
Separations in the mercury column of
thermometers
Old and worn electrical cords
Handling liquid chemicals
Avoid measuring volumes of strong acids and alkaline solutions at eye level

Reagent in dropper bottles


Reagent in a stopper bottle
Mixing
Always add concentrated chemical to water
rather than water to chemical.
Pipetting
Heating
Disposal
Chemical spills in the laboratory
notify your instructor

Non volatile and non flammable


materials
Wipe with absorbent cloth then
wipe the contaminated area with
detergent and water and the area
should be mopped dry.
xVolatile, flammable and toxic spill
materials
xAcid spills- apply neutralizer
Chemical spills on a person
Over the body

On a small area of body

In the eyes


Burns

Fire

Injury or Illness
EXPERIMENT NO. 1
COMMON LABORATORY APPARATUS
Objectives:
1. Recognize common laboratory
apparatus and know their uses
correctly.
2. Draw the different set ups for
evaporation of liquids, filtration,
titration, ashing, and separation of
two immiscible liquids and label all
the parts of the equipment and
glasswares used.
Different Separation Techniques:
1. Direct Evaporation by Heat
2. Filtration
 is any of various mechanical, physical
or biological operations that separate
solids from fluids (liquids or gases) by
adding a medium through which only
the fluid can pass.
 The fluid that passes through is called
the filtrate.
 Separation of solid by pouring the liquid
through a sieve or filter paper
3. Titration
 A laboratory procedure for quantitative
analysis
 A method or process of determining the
concentration of a dissolved substance in
terms of the smallest amount of reagent of
known concentration required to bring
about a given effect in reaction with a
known volume of the test solution.
4. Ashing
An analytic procedure in which
a sample is heated in a
furnace to leave a residue
of ash, either to determine
the gross mineral content,
or as a preparation for
further analysis
5. Separation of two
immiscible liquids
The liquids have clearly 2 distinct
phases the bottom liquid may be
drained from the separatory
funnel.
6. Simple Distillation
 Used to purify liquids and to separate
one liquid from another. It is based on
the difference in the physical property of
liquids called volatility