SELECTIVE TOXICITY

TEST OF BEHAVIOR
Why study behavior
Behavior provides a unique perspective
linking the physiology and ecology of an
organism and its environment: (Little and
Brewer, 2001). Behavior is both a
sequence of quantifiable actions, operating
through the central and peripheral nervous
systems (Keenleyside, 1979), and the
cumulative manifestation of genetic,
biochemical, and physiologic processes
essential to life, such as feeding,
reproduction and predator avoidance.
Why study behavior
Behavior allows an organism to adjust to
external and internal stimuli in order to best
meet the challenge of surviving in a hanging
environment. Conversely, behavior is also
the result of adaptations to environmental
variables. Thus, behavior is a selective
response that is constantly adapting through
direct interaction with physical, chemical,
social, and physiological aspects of the
environment.
TEST OF TOXICITY OF COMBINATION OF ETHANOL
EXTRACT OF SOYBEAN SEEDS (glycine max L. Merr)
DETAM-1 VARIETY AND JATI BELANDA LEAVES
(guazuma ulmifolia) ON WISTAR RAT BEHAVIOR
Toxicity Test
01 Subchronic toxicity tests evaluate the effects of test
chemicals given repeatedly for approximately 90 days
(10% of normal rat life). In addition, the purpose of the
subchronic toxicity test is to determine a dose that does
not cause a toxic effect (NOAEL / No Observed Adverse
Effect Level), also studies the cumulative effect and the
reversibility of the substance. The principle of this toxicity
test is that test chemicals are administered in several
dosage levels in several groups and added by satellite
groups to see any delayed effects or reversible effects
(BPOM, 2014). In the subchronic toxicity study,
observations were carried out for 90 days whether there
were symptoms of toxicity in mice that included motor
activity, changes in skin, eyes, autonomic activity
(lacrimation, catalysis, salivation, piloerection, pupil size,
breathing), grooming, strange behavior (strange self-
mutilation, walking backwards), mortality and others
(OECD, 2008).
 Toxicity Test
Subchronic toxicity tests evaluate the effects of test
chemicals given repeatedly for approximately 90 days
(10% of normal rat life). In addition, the purpose of the
subchronic toxicity test is to determine a dose that does
not cause a toxic effect (NOAEL / No Observed Adverse
40% 80% 60% 50% Effect Level), also studies the cumulative effect and the
reversibility of the substance. The principle of this toxicity
test is that test chemicals are administered in several
dosage levels in several groups and added by satellite
groups to see any delayed effects or reversible effects
(BPOM, 2014). In the subchronic toxicity study,
observations were carried out for 90 days whether there
were symptoms of toxicity in mice that included motor
activity, changes in skin, eyes, autonomic activity
(lacrimation, catalysis, salivation, piloerection, pupil size,
breathing), grooming, strange behavior (strange self-
mutilation, walking backwards), mortality and others
(OECD, 2008).
Behavior
Male Wistar Rat
01 Rat activity on the platform (raising his body, looking up,
looking down, and crossing the line decreased on
observations of 91 and 121 days, but still within normal
limits). Motor activity, posture, and respiration of male
rats in each group were within normal limits both before
and after treatment, none of the mice gave symptoms
of toxicity such as straub, piloerection, ptosis,
excessive lacrimation, catalepsy, excessive salivation,
vocalization, tremor, convulsions, and whrithing. 0-20%
mortality. Male pineal and corneal reflexes were found
to be positive in each group (100%) before and after
treatment. Likewise for the observation of hanging
activity, retention, flexion and hafner of male rats in
each group gave 100% results before and after
treatment. Grooming on average decreased after
treatment, while defecation and urination increased.
02 Female Wistar Rat
Rat activity on the platform (raising his body, looking up,
looking down, and crossing the line decreased at
observations of days 91 and 121, but still within normal
limits. Motor activity, body posture, and respiration of
female rats in each group within normal limits both
before and after treatment, none of the mice gave
toxicity symptoms such as straub, piloerection, ptosis,
excessive lacrimation, catalysis, excessive salivation,
vocalization, tremor, convulsions, and whrithing, 0-20%
mortality, pineal reflexes. and corneal female mice were
found positive in each group (100%) before and after
treatment, as well as for observing the hanging,
retractable, flexion and hafner activities of female mice
in each group giving 100% results before and after
treatment. decrease after treatment, while defecation
and urination have increased.
Discussion
Observation of Wistar rat behavior during treatment did not
occur symptoms of toxicity which include motor activity,
changes in skin, eyes, autonomic activity (lacrimation,
catalysis, salivation, piloerection, pupil size, breathing),
grooming, strange behavior (self-mutilation, walking
backward), mortality and others before, during and after
treatment. This shows that the administration of ethanol
extract of Detam-1 soybean seeds and Dutch teak leaves
for 90 days did not cause toxic effects.
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