APPLICATION OF BACTERIA AS

SELF HEALING AGENT IN SELF

COMPACTING CONCRETE

Presented By: Under Guidance of:

FAIZAN ALI Mr. Nishant Kumar
180136006 Assistant Professor
M.Tech in Civil Engineering (Structural Department of Civil Engineering
Engineering)
 CONTENTS
 Objectives
 Methodology
 Experiments performed
 Literature review

USE OF BACTERIA FOR SELF HEALING OF CONCRETE 18-12-2019

 OBJECTIVES
 To prepare self compacting as per EFNARC guidelines.
 To induce calcite precipitating bacteria in concrete mix
and measure the physical mechanical properties of the
mix and self healing capacity of the mix.
• Isolation of bacteria.
• Identification of bacteria.
• Concrete sample preparation with bacteria.

 To measure the self healing capacity of the sample

casted.

USE OF BACTERIA FOR SELF HEALING OF CONCRETE 18-12-2019

 METHODOLOGY

SCC MIX DESIGN AS ISOLATION & CAST CONCRETE

PER IS.10262:2019 ENRICHMENT SAMPLES WITH VARIATION
OF BACTERIA OF BACTERIA %

TEST CASTED SAMPLE FOR SELF

HEALING

USE OF BACTERIA FOR SELF HEALING OF CONCRETE 18-12-2019

 TESTS ON DRY MIX. MATERIALS OF CONCRETE

Fine Cement
Water Coarse
Aggregate
Aggregate

PH. = 10 • IS-sieve analysis • IS-sieve analysis • Specific

as per is 383- as per 383-1970 Gravity test
1970.
• Specific gravity • Standard
• Specific gravity test. consistency
test. test.
• Water
• Water absorption test • Initial and
absorption test final setting
• Moisture content time of
• Moisture content test cement
test

18-12-2019

USE OF BACTERIA FOR SELF HEALING OF CONCRETE

Determination of specific gravity of Fine
aggregate

 Weight of empty bottle (w1)= 666 (gm.)

 Weight of empty bottle + Fine aggregate (w2)=852 (gm.)
 Bottle + Fine aggregate + Water (w3)=1666(gm.)
 Weight of empty bottle+ water (w4)=1552(gm.)

(W2 – W1)
Specific Gravity=
(W2 – W1)-(W3 – W4)

=> Specific gravity of fine aggregate= 2.58

Sp.G ranges from 2.5 – 3.0 as per IS-383

USE OF BACTERIA FOR SELF HEALING OF CONCRETE 18-12-2019
Determination of specific gravity of Coarse
Aggregate

 Weight of empty bottle (w1)= 666 (gm.)

 Weight of empty bottle + Coarse aggregate (w2)=852 (gm.)
 Bottle + Coarse aggregate + Water (w3)=1666(gm.)
 Weight of empty bottle+ water (w4)=1552(gm.)

(W2 – W1)
Specific Gravity=
(W2 – W1)-(W3 – W4)

=> Specific gravity of coarse aggregate= 2.78

Sp.G ranges from 2.5 – 3.0 as per IS-383

USE OF BACTERIA FOR SELF HEALING OF CONCRETE 18-12-2019
 Moisture content of Fine Aggregate
 weight of surface dried aggregate (W1) = 28(gm.)
 Weight of oven dried aggregate (W2)= 27 (gm.)

Moisture content={(W1-W2)/W2}*100

• Moisture content of fine

aggregate =3.7%

USE OF BACTERIA FOR SELF HEALING OF CONCRETE 18-12-2019

 Moisture content of Coarse Aggregate
 weight of saturated surface dry aggregate(W1) = 40 (gm.)
 Weight of oven dried aggregate (W2)=37(gm.)

Moisture content={(W1-W2)/W2}*100

• Moisture content of coarse

aggregate =8.1%

USE OF BACTERIA FOR SELF HEALING OF CONCRETE 18-12-2019

 Water absorption of fine aggregate
 Weight of surface dried aggregate=120gm
 Weight of aggregate after immersed in water for 24 hrs.=161gm

Water absorption={(w2-w1)/w2}*100

• Water absorption of fine aggregate=25.4%

USE OF BACTERIA FOR SELF HEALING OF CONCRETE 18-12-2019

 Water absorption of coarse aggregate

 Weight of surface dried aggregate=370gm

 Weight of aggregate after immersed in water for 24 hrs.=379gm

Water absorption={(w2-w1)/w2}*100

• Water absorption of coarse aggregate=2.3%

USE OF BACTERIA FOR SELF HEALING OF CONCRETE 18-12-2019

IS-SIEVE ANALYSIS OF FINE AGGREGATE
 1 KG of fine aggregate sample was taken and air dried
for some time

Sieve Weight of % of total Cumulative % Passing

Size Aggregate wt retained of total wt %
(mm) Retained (gm) retained
10 0 0 0 100
4.75 7 0.7 0.7 99.3
2.36 11 1.1 1.8 98.2
1.18 31 3.1 4.9 95.1
0.6 587 58.7 63.6 36.4
0.3 319 31.9 95.5 4.5
0.15 39 3.9 99.4 0.6
Pan 6 0.6 100 0

• Values conforming to zone IV as per IS-383

USE OF BACTERIA FOR SELF HEALING OF CONCRETE 18-12-2019

 SIEVE ANALYSIS OF COARSE AGGREGATE
 1 kg sample of coarse aggregate was taken and air dried for some
13 time.

IS Weight of % of Cumulative % of Passing %

Sieve Aggregate total wt total wt retained
Size Retained retained
(mm) (gm)
20 0 0 0 100
16 273 27.3 27.3 72.7
12.5 422 42.2 69.5 30.5
10 231 23 92.5 7.5
4.75 85 7.5 100 0
Pan 0 0 100 0

• Max nominal Coarse aggregate size= 20 mm

Specific gravity test of cement sample
Observation Trial 1 with diesel Trial 2 with kerosene
Wt. of empty bottle 675 (gm.) 130 (gm.)
(W1)
Wt. of bottle + cement 926 (gm.) 162 (gm.)
(W2)
Wt. of bottle + cement 1590 (gm.) 255 (gm.)
+oil (W3)
Wt. of bottle +oil (W4) 1413 (gm.) 230 (gm.)

Wt. of bottle + water 1574 (gm.) 1552 (gm.)

(W5)
Specific gravity of cement is given by:
{(w2-w1)*(w4-w1)}/{[w4-w1)-(w3-w2)]*(w5-w1)}

Sp. Gravity of cement=3.1

using diesel & 3.15 using
kerosene Ranges from 3.1-3.25 as
per IS-5513-1976
USE OF BACTERIA FOR SELF HEALING OF CONCRETE 18-12-2019
 Normal consistency of
cement
Trial Wt. Of cement % of H2O of dry Amount of H2O Penetration
no (gm.) cement added (ml) (mm)
1 300 26% 78 22
2 300 28% 84 15
3 300 30% 90 6

Consistency of cement =30% as penetration of

plunger ranges from 5 to 7mm from bottom as per
IS- 5513-1976

USE OF BACTERIA FOR SELF HEALING OF CONCRETE 18-12-2019

 Initial and final setting
time of cement
 Initial setting time of cement was recorded as 40 minutes
And 27 seconds.
 Final setting time of cement was recorded as 48 minutes and
23 seconds.

USE OF BACTERIA FOR SELF HEALING OF CONCRETE 18-12-2019

Tests for self compacting
concrete
 Slump test.
 Passing ability by L-box test.
 V- funnel flow time.
 U- box test.

USE OF BACTERIA FOR SELF HEALING OF CONCRETE 18-12-2019

Slump test
Trial 1

USE OF BACTERIA FOR SELF HEALING OF CONCRETE 18-12-2019

 Slump flow class SF2.
 Slump ranges from 660mm- 750mm.
 Slump flow was found to be 26 inches which is greater
than 660 mm.
 SF2 is suitable for normal applications.

USE OF BACTERIA FOR SELF HEALING OF CONCRETE 18-12-2019

USE OF BACTERIA FOR SELF HEALING OF CONCRETE 18-12-2019
IS-Codes references

 For finess modulus of aggregate: IS-383-1970.

 For specific gravity of aggregate: IS-2386 part-3.
 For normal consistency & specific gravity of cement: IS-
5513-1976, &4031(part 4).
 Moisture content and water absorption of aggregate:
IS:2386
 Specific gravity of cement: IS-5513-1976.
 Fly ash conforming to IS-3812.

USE OF BACTERIA FOR SELF HEALING OF CONCRETE 18-12-2019

USE OF BACTERIA FOR SELF HEALING OF CONCRETE 18-12-2019