DENGUE FEVER

Prof. Dr. Muhammad Ali Khan

MBBS, DCH, MRCP (UK)

Secretary DEAG Punjab

Ex Head Department of Pediatrics

SIMS/Services Hospital Lahore
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 Dengue Virus
 Family : Flaviviridae
 Genus : Flavivirus
 Serotypes : DV1, DV2, DV3, DV4

 Enveloped virus
 3 major proteins
 SS positive sense RNA

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Dr. S Guanasena
 Viral Serotypes
 DV1
 DV2
 DV3
 DV4
 Subgroups and clades
 One or more virus types in
circulation during an epidemic

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Spectrum of Disease
Clinical course of
Dengue
 Dengue Infection

Clinically significant Dengue infection may be

1. Simple dengue fever (DF)

2. Dengue hemorrhagic fever (DHF)

(plasma leak <5%)

3. Dengue Shock Syndrome (DSS)

(plasma leak >5%)
 Clinical Features – DF
 Fever > 2 and < 10 days (essential criterion)
 Headache
 Retro orbital pain
 Myalgia
 Arthralgia/ severe backache/ bone pains
 Rash
 Bleeding manifestations (epistaxis, hematemesis,
bloody stools, menorrhagia, hemoptysis)
 Abdominal pain
 Decreased urinary output despite adequate fluid
intake
 Irritability in infants
DHF
or

DLF
 SHOCK
A complex clinical syndrome
of decreased blood flow
to body tissues
resulting in
cellular dysfunction and
eventual
organ failure
 Plasma leaks -
Pathophysiology
 Host response
 Subsequent infection

 Previous IgG
 Neutralizing (protective)
 non neutralizing (replication enhancing)

 Increased viremia increased TNF,

interferon, interleukin-2 and
hypocomplementemia
endothelial injury and increased leakage

 Viral response
 Pathophysiology of DHF
 Increased capillary permeability
 Protein rich fluid exudes into the interstitial space - Pleural effusion ,
Ascites etc.

 Circulatory volume collapses – SHOCK

 Sympathetic over activity

 Vasoconstriction, tachycardia

 Loss of volume
 Reduces pulse pressure

 Blood becomes thick due to loss of fluid

 Rising hematocrit and delayed capillary filling

 Compromised renal and hepatic perfusion –

 Reduced urine output and tender hepatomegaly
 Patho-physiology of DHF
 Management of DHF during the critical stage is that of
Shock

 But there is one important consideration:

Fluid is not being lost out but it is going to 3rd space and will be
resorbed back
so
Over-enthusiastic fluid replacement

during the critical phase – when the fluid is oozing out - would result
in

fluid over load during the recovery phase

 Patho-physiology of DHF

 People do not die of hemorrhage in DHF

 They die
 Either due to shock and 20 organ failure

 Or due to Pulmonary edema & fluid over load during

the recovery phase
End organ failure in DHF

Approximate outcomes
Single organ failure - mortality = 40%

Two organ Failure - mortality = 80%

Three organ failure - mortality =

>99%
 Case review
( of 9 deaths at Services
Hospital )

 8 died of profound shock and associated end

organ failure

 Only one had suspected CVA as a cause of

death
 Clinical course of
DHF

 Course of simple Dengue fever is

generally uneventful and non-fatal

 Whereas DHF can be life-threatening

Differentiating DF from DHF is critical

 Clinical course of
DHF

 Both the DF and DHF can have bleeding

tendencies therefore:
Bleeding is not the differentiating point
between the two
 Clinical course of
DHF

 Clinical course can be divided into

three stages

1. Febrile Phase
2. Critical Phase

3. Recovery Phase
 Clinical course of
DHF

Febrile Phase

 High fever
 Some petechial hemorrhages
 With generalized aches & pains and
headache;
 this usually lasts two to seven days

People generally don’t die during this stage

 Clinical course of
DHF

Critical Phase
 Starts with the resolution of fever
 Occurs in a few people
 Lasts for just 24-48 hours or so
 Is associated with plasma leak – volume depletion
& shock

This is the phase where management is critical

 Clinical course of
DHF

 DHF is classically associated with

 Plasma leak into the 3rd compartment

 And circulatory compromise
Signs of plasma leak in
DHF
 Patient is not feeling well with resolution of
fever

Warning signs
 Low pulse pressure <20 mm

 Low urine output

 Delayed capillary filling

 Tender hepatomegaly
Signs of impending DHF

Warning signs (lab reports)

 Increasing hematocrit

 Edema of the gall bladder, ascites or

pleural effusion
 Low albumin

 Low cholesterol

 Acute fall in platelet count

Highly Suggestive of DHF
 Disproportionate tachycardia
 Narrowing of pulse pressure < 20
mm
 CRFT > 2 secs
 Tender hepatomegaly (DHF likely)
Confirmed DHF**
 Ascites on U/S
 Pleural effusions (CXR Right lateral
decubitus or chest U/S to detect
minimal effusion)
** Definitive evidence of plasma leakage

 Haemoconcentration
HCT 20% rise from baseline or rise
approaching 20% if patient already
on IV fluids
 Biochemistry
o Serum albumin < 3.5 g/dl or 0.5
gm/dl fall during illness
 Non fasting serum cholesterol < 100
mg/dl or 20mg/dl fall during illness
 Oedematous gall bladder wall on U/S

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 Rate of Fluid loss and
intake (Critical Phase)
 Clinical course of
DHF

Recovery Phase

 Volume gets resorbed

 Volume over-load may occur

This is the phase where people die because

of the problems faced during the critical
stage
Lab Diagnosis
 IgG antibody - specific to
the initial infecting DV
serotype + cross reacting
antibody

IgM antibody to the

secondary infecting DV
serotype

Following primary infection –

Specific antibody response + CMI (memory T cells)
Cross reactive antibody response + CMI (memory T cells)
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Dr. S Guanasena
 Investigations
 CBC and platelet count

 First 5 days
 NS 1
 PCR

 >5 days
 IgM
 IgG
 Update on vaccine
 Chimera vaccine  Attenuated vaccine
 Yellow fever &  6-8 cycles in DKC
dengue (Dog kidney cells)
 Launched 2016
 Partial immunity
 Risk of secondary
infection
 HAVE WE DONE
ENOUGH?

Asia pacific strategic plan for control of Dengue

(2010-2015)

 Decrease prevalence by
10% per year
What are our Goals?????
 Keep DHF mortality below
1%
 Current situation in
Punjab
Lahore 2011 Rawalpindi 2015

 DEN 2  DEN 1,2,3,4

 Innumerable
 2349 cases (uptill 27-
10-2015)

 Few DHF  > 300 cases of DHF

and DSS

DEAG TRINED MEDICS AND

 10 deaths
PARAMEDICS = 13000
 Current situation in
Punjab
Lahore, 2016 Rawalpindi, 2016

 DEN 1  DEN 1,2,3

 109
 299
 Few DHF
 18
 No death

 03 deaths

DEAG TRINED MEDICS AND

PARAMEDICS = 3165
 Take Home message

 We can prevent quite a few deaths ---- if

we can

1. Differentiate DF from DHF

2. Diagnose the onset of critical phase
(phase of leaking)
3. Give appropriate fluids