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Definition
Principle
Separation
HPLC Applications
HPLC
Kata kromatogrfi berasal dari bahasa Greek yaitu Chromato dan grafe yaitu
penulisan dengan warna (writing with colors).
Prinsip: kromatografi merupakan cara pemisahan yang mendasarkan pada
partisi cuplikan antara fase bergerak (mobile phase) dan fase diam (stationary
phase).
Definition
High-performance liquid
chromatography is a technique
in analytical chemistry used to separate,
identify, and quantify each component in a
mixture. It relies on pumps to pass a
pressurized liquid solvent containing the
sample mixture through a column filled
with a solid adsorbent material
Chromatography
Increasing strength
Light leaf
Water flow
Heavy stone
Base
Mobile Phase / Stationary Phase
Mixer mAU
Pumps
Column
Detector
Solvents
Mixer mAU
Pumps
Column
Detector
Solvents
Separations
Injector Chromatogram
Mixer mAU
Pumps
Column
Detector
Solvents
Separations
Injector Chromatogram
Mixer mAU
Pumps
Column
Detector
Solvents
Separations
Injector Chromatogram
Mixer mAU
Pumps
Column
Detector
Solvents
Separations
Injector Chromatogram
Mixer mAU
Pumps
Column
Detector
Solvents
Separations
Injector Chromatogram
Mixer mAU
Pumps
Column
Detector
Solvents
Separations
Injector Chromatogram
Mixer mAU
Pumps
Column
Detector
Solvents
Separations
Injector Chromatogram
Mixer mAU
Pumps
Column
Detector
Solvents
Separations
Injector Chromatogram
Mixer mAU
Pumps
Column
Detector
Solvents
Separations
Injector Chromatogram
Mixer mAU
Pumps
Column
Detector
Solvents
Separations
Injector Chromatogram
Mixer mAU
Pumps
Column
Detector
Solvents
Separations
Injector Chromatogram
Mixer mAU
Pumps
Column
Detector
Solvents
The Chromatogram
tR
mAU Area or height is proportional
to the quantity of analyte.
to
Injection time
Jenis Detektor HPLC
UV-Vis Detector
Photodiode array Detector (PDA = DAD)
Refractive Index Detector (RID)
Fluorescence Detector (FLD)
Evaporative Light Scattering Detector (ELSD)
Electrochemical Detector (ECD)
Conductivity Detector
Radiometric Detector
Hyphenated Systems
LC-MS;
LC-MS/MS; LC-NMR
PERHITUNGAN HPLC
Parameter
Area Theoritical Resolution Retention
HETP K'
Plate (Rs) Time (Rt)
67043 4944.345 50.563 427.488 3.646 4.322
267071 4742.723 52.712 426.925 3.198 4.332
465446 3450.100 72.462 433.095 2.892 4.341
MIN MIN
2000 1,8
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