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Danish Faraz
Msc(Hons) Entomology
2nd semester
2014-ag-4951
Supervisor:Dr.Dildar Gogi
sb
Topic:
It is notorious pest of different fruit crops mainly Citrus & also different
vegetables.
Our country is facing losses of at least $200 million annually owing to fruit fly
attacks which is serious threat to the export of fruits.
Fruit fly can be Control through pheromonal traps like methyl uginol which js
majnly sex pheromonal trap .
Most effective pesticide against fruit fly is Dipterex.which mainly consists of
Trichlorofon & methyl uginol.
But we can also control fruit fly movement & we can manage it through
microflora present in the fruit fly gut.
There are different genera of bacteria are present in the gut of fruit fly.
We should isolate & identify these bacteria for proper study how these
bacteria are helpful in the management of fruit fly.
Isolation of bacteria
Fruit flies that have their natural gut microbes were less active than flies that
lack the gut bacterium Lactobacillus brevis.
Enzyme modifies certain sugars, which leads, by an unknown process, to a
decrease in the level of the sugar trehalose in the body of flies in which this
bacterial enzyme is present.
which a decrease in trehalose is accompanied by a decrease in the activity of
octopaminergic neurons (those that produce the neurotransmitter molecule
octopamine) that regulate fly locomotion flies that have their natural gut
microbes.
Flies lacking Lactobacillus brevis have higher levels of the sugar trehalose in
their body and are proposed to have higher activity of octopaminergic neurons.
These gut bacteria influence the behavior including movement.Fruit flies
which do not carry bacteria are hyperactive move faster over greater distance
& took shorter rests.
Locomotion is important for a number of activities such as mating and
searching for food.
When germ free flies received L.brevis their movement slow down.
Through octopamine these flies can produce nerve cells which helps in the
normal movement but these bacteria turn off this octopamine so they cannot
move faster for foraging or mating .
Bacterial Bait preparation
The predominant bacterial symbionts in the gut of fruit flies, Bactrocera spp.
Washed and fermented bacterial preparations of these symbionts were
evaluated for their attractancy to fruit fly species (Bactrocera cucurbitae) under
laboratory and field conditions.
P. agglomerans (washed bacterial preparation) in combination with sugar
attracted maximum number (mean 11.33 adults/30 min) of B. cucurbitae, while
protein hydrolisate in combination with sugar .
Combination of washed bacteria proved superior to control in terms of
attractancy for both species.
Fermented bacterial prepration ,k.oxytoca In combination with jaggary attracted
to maximum fruit flies when applied in lab condition on potted plants.
This fermented bacteria can also used in field condition on foliar application
and as bait in combination with different insecticides resulted in significance
reduction in fruit fly infestaion over 65%.