Pemanfaatan Metabolomik dan

Kemometrik dalam Kendali Mutu

Obat Herbal
Teknik spektroskopi UV-Vis dan IR
sebagai metode kendali mutu
tumbuhan obat
Menggunakan
program pada
komputer
Langkah utama untuk analisis sampel:
a. Preparasi sampel
b. Setting instrumental
c. Pembuatan spektrum
d. Pemrosesan data
Preparasi sampel
Setting instrumental
Pembuatan spektrum
METABOLOMIK
 Main purpose
• To introduce metabolomics as the new
unconventional approach in solving problems
related to pharmacognosy.
• To explain the basic concepts of metabolomics-
based research.
• To expose the students to the types of
instrument platforms that can be used in
metabolomics-based investigation.
• To expose to the students the types of research
closely related to pharmacognosy.
• The current rate of discovery and
development or a new drug is estimated at
US$0.81.8 billion
• metabolite fingerprinting and metabolite
profiling
• Typical steps in metabolomics investigation
include experimental design, data acquisition,
data processing, data pretreatment,
exploratory analysis/unsupervised modeling,
supervised modeling, metabolites
characterization and quantification, and
finally, the interpretation.
• Factors that should be considered in the study
design include, e.g., the variations due to age,
parts of the organism, growth condition, diurnal
variation, gender, animal strain, diet, and stress.
• The time gap between sampling and freezing or
extraction may also alter the metabolite profile
due to protraction of residual biochemical
reactions.
• All of these issues can significantly modify the
metabolite profile obtained from the
experimental subjects.
 Protokol proses
1. Wu H, Southam AD, Hines A, Viant MR. High-
throughput tissue extraction protocol for NMR-
and MS-based metabolomics. Anal Biochem.
2008;372:20412.
2. Beckonert O, Keun HC, Ebbels TM, Bundy J,
Holmes E, Lindon JC, et al. Metabolic profiling,
metabolomic and metabonomic procedures for
NMR spectroscopy of urine, plasma, serum and
tissue extracts. Nat Protoc 2007;2:2692703.
1. Describe the basic concept of metabolomics as a tool
for research in pharmacognosy.
2. Describe the reasons why metabolomics could
become useful in understanding the holistic concept
of herbal medicine.
3. Explain the rationale behind the belief that
metabolomics approach is different from the
conventional methods in pharmacognosy.
4. What are the factors that can cause variations in
metabolomics analysis?
5. Describe the way to develop a potent medicinal plant
into a herbal drug.