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Martin R. Hackman
NJ- DEP
Office of Quality Assurance
H igh
Performance
L iquid
C hromatography
H igh
Pressure
L iquid
C hromatography
H igh
Priced
L iquid
C hromatography
HPLC Methods
• Parameter Group Method Compounds
2,4,5-T
Benzidine
Fluorene
Compounds
TNT (2,4,6-Trinitrotoluene)
H2C=CH-CN Acrylonitrile
Carbaryl
Partitioning
• Separation is based on the analyte’s relative
solubility between two liquid phases
• Reverse Phase.
- Non-polar stationary phase and a polar
solvent.
Common Reverse Phase Solvents
• Methanol CH3OH
• Acetonitrile CH3CN
• Tetrahydrofuran
• Water H 2O
Columns
• Solid Support - Backbone for bonded phases.
– Usually 10µ, 5µ or 3µ silica or polymeric particles.
• Bonded Phases - Functional groups firmly linked
(chemically bound) to the solid support.
– Extremely stable
– Reproducible
• Guard - Protects the analytical column:
– Particles
– Interferences
– Prolongs the life of the analytical column
• Analytical - Performs the separation.
Bonded Phases