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(LAB TUTOR)
Herrera, M. | Macatingrao, S. | Padilla, J. | Tan, M.
Group 10
4b6
INTRODUCTION
Photo Retrieved from: https://step1.medbullets.com/neurology/113052/action-potential-basics
Compound Action Potentials
METHODOLOGY
Dissection
Nerve Bath
Photo retrieved from https://openwetware.org/wiki/Lab_9:_Conduction_Velocity_of_Nerves
Exercise 1: threshold voltage and maximal cap amplitude
Set the
stimulus
voltage to 10mV
Click START to
stimulate the
nerve
Click the UP arrow
in the Stimulator
Panel to increase
stimulus voltage
by 10 mV and then
Repeat step 3
click START
until you reach
a stimulus
voltage of
400mV
Exercise 2: refractory period
Set the Stimulus voltage
to the minimum voltage Click START to
required to elicit a stimulate the nerve
maximal CAP (Exercise 1), twice at the selected
then set stimulus interval interval
to 4.0ms
Exercise 3 ● The conduction velocity of the frog’s sciatic nerve was measured to be 125 m/s
○ Higher than the normal range for a frog’s nerve fiber in the lab = 40 m/s.