Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
• Objectives
To understand the theory and practice of monitoring the bacteriological
quality of water.
To know the typical bacteriological standards required for Raw and
Treated Water.
References
Water Supply - Twort et al
Examination of Water for Pollution Control (Vol 3) - M.J. Suess
Report 71 - The microbiology of water 1994 Part 1 - Drinking Water ,HMSO
EC Directives
(98/83/EC) Drinking Water - Official Journal L 330
(80/778/EEC) Drinking Water - Official Journal L229
(75/440/EEC) Abstraction - Official Journal L 194
Water Supply Bacteriology
useful for:
(1) Detection of faecal pollution in potential water supply (very sensitive test).
(2) Assessment of water treatment plant performance.
(3) Confirmation of hygienic safety of final water entering supply.
(4) Surveillance of water quality throughout distribution.
(5) Indicator bacteria: give Quantitative results therefore used as basis for these
standards:
Coliform/100 ml
Tap Water 0
Bacterial Examination of Water
(4) Quantitative result therefore can define microbiological standards for different
water uses.
Indicator Organisms
• 6. Harmless to humans
Useful test for water supplies which can only be sampled occasionally and which
may have intermittent pollution (e.g. shallow wells/springs)
Also useful for examining marine sediments to detect zone affected by sewage
outfall. (Range > 10 km)
Raw Water (Abstraction) Standards
1962 WHO (Omitted 1973 and 1984)
Coliform/100ml Classification
0-50 I Needs Disinfection only
50-5,000 II Needs Conventional treatment
5,000-50,000 III Needs Extensive treatment
>50,000 IV Unacceptable - only use if
nothing else
1975 EEC
A1 A2 A3
Coliforms/100 ml 50 5,000 50,000
Faecal Coliforms/100 ml 20 2,000 20,000
Faecal Streps./100 ml 20 1,000 10,000
Salmonellae Nil/5 l Nil/5 l
DisinfectionConventional Intensive
Only Treatment Treatment
Conventional Treatment
- flocculation, coagulation, decantation,
filtration, chlorination
Intensive Treatment
- breakpoint chlorination, flocculation, coagulation,
decantation, filtration, carbon adsorption, disinfection (ozone, final chlorination)
Typical Bacterial Counts for River
Thames at Water Intake
Total ‘aquatic’ bacteria (20 C)1.2 x 106 /100 ml
Total ‘Coliform’ bacteria (37 C) 1.6 x 105 /100 ml
Problems -
(1) Aftergrowth/regrowth
(2) Slime formation
(3) Contamination of Distribution System
(4) Resistance of viruses
WHO Guidelines 1984
Bacteriological Quality Treated Water
Piped supplies CFU/100 ml
EC Directive (80/778/EEC)
• same rate as to WHO (but up to 300,000 pop)
• no increments above 300,000 pop
At Treatment Works
WHO (1984)
daily testing for total coliforms
EC Directive (80/778/EEC)
no requirements
UK Regulations, Water Supply Regulations 2000
Directive and National Requirements
Concept of Supply Zones, fixed annually, ideally linked to a Supply Point
(i.e.WTW, pumping station, blending point, service reservoir).
Microbiological Quality
(at Consumers taps)
Sample (M.F.) PCV
Total Coliforms 100 ml 0
Escherichia coli 100 ml 0
Enterococci 100 ml 0
Clostridium perfringens 100 ml 0
Colony count - at 22C and 37C (no abnormal change)
( in Bottled water)
Enterococci and E. coli (0/250ml)
Pseudomonas aeruginosa (0/250ml)
Colony count (22C < 100/ml; 37C < 20/ml)
UK Regulations, Water Supply Regulations 2000
Frequency of monitoring
Audit and Check monitoring, Standard and Reduced (where deterioration is negligible)
Frequencies . Therefore frequency varies widely, sliding scales based on:
• Output of WTW (m3/day)
• Size of Supply Zone (<100 to 100,000 p.e)
* these termed “Indicator Parameters” (Schedule 2), any failures require further
investigation. Rest are “Prescribed Concentration or Value (PCV)” , (Schedule 1).
Note ; residual disinfectant monitored at taps and works at the same frequency as TC.
Bacterial Removal
% surviving 100
37C Plate Count
Storage
10 Reservoir
E. coli 12C
1
E. coli 16C
0.1
10 20 30 40
time (d)
Storage
After 30 days, assume 90% removal E.coli
Disinfection (Cl2)
Assume further 99% removal
(4) Disinfectant
Failure to maintain residual throughout the distribution system.
Enforcement of Standards:
• Drinking Water Inspectorate (DWI)
– formed 1990
– monitors water undertakers’ performance
• Water Undertaker
– analyse samples and keep records
– Produce reports
– Investigate outbreaks (+ inform DWI)
• Identify source (e.g. consumer’s installations or supplies?)
• Possible failure
TC but not FC in supply
– Possible Causes: sludge in pipes, nutrients in water, dead-ends (low chlorine
residual)
• Solutions
– distribution system checks
– flush network
– switch raw water source
– increase disinfectant strength
Health guidelines for the use of wastewater in agriculture and aquaculture.
Technical Report Series 778, WHO, Geneva, 1989
B. Restricted Irrigation. (Only crops which are 1 or less helminth egg per litre
not eaten or not eaten raw, cereals, fodder .
crops, pasture and trees)