Experiment Questions

Enzyme Temperature
 Enzyme Temperature
State one factor that you kept constant during
the investigation.

pH or substrate conc. or enzyme conc.

 Enzyme Temperature
How did you vary the temperature?

Water baths (at different temps.)

 Enzyme Temperature
How did you know that the enzyme had
completed its activity?

No more product/ Colour change/ No more

bubbles/ No more foam
 Enzyme Temperature
Name the enzyme that you used.

Name of enzyme, e.g. catalase

 Enzyme Temperature
Name the substrate of the enzyme that you
used.

Name of matching substrate, e.g. hydrogen

peroxide
 Enzyme Temperature
A number of factors were kept constant during
this experiment. Explain how you kept one of
them constant?

pH—used a buffer
substrate conc.—same volume
enzyme conc.—same amount
 Enzyme Temperature
How did you measure the rate of activity of the
enzyme?

Time / change e.g. colour, foam, etc

or data logger / sensor named
 Enzyme Temperature
What was the result of your investigation?

Activity varies with temperature or reference to

activity at a particular temperature
 Enzyme Temperature
(i) Name an enzyme that you used.
(ii) Name the substance that this enzyme acted
on.

(i) Catalase or amylase

(ii) H2O2 or starch (must match enzyme)
 Enzyme Temperature
How did you vary the temperature?

Water baths/ at different temperatures/

thermostat
 Enzyme Temperature
How did you measure the rate of the enzyme’s
action?

Volumes of foam/ per minute/ disappearance

of blue colour/ per minute
 Enzyme Temperature
(i) What enzyme did you use?
(ii) What substrate did you use?

Name of (i) enzyme, e.g. catalase (ii) substrate,

e.g. H2O2 —must match
 Enzyme Temperature
How did you know that the enzyme had
completed its activity?

No more product /colour change / no more

bubbles / no more foam
 Enzyme Temperature
How did you vary the temperature in your
experiment?

Water bath / different temperature treatments

/ Bunsen / thermostat
 Enzyme Temperature
(i) Name the enzyme that you used.
(ii) Name the substrate with which the enzyme
reacts.

(i) Name of enzyme

(ii) Name of substrate [must match enzyme]
 Enzyme Temperature
How did you vary the temperature?

Water bath or ice or thermostat

 Enzyme Temperature
How did you keep a constant pH during the
investigation?

Buffer
 Enzyme Temperature
How did you measure the rate of activity of the
enzyme?

Time / change (colour, height of foam, etc.) OR

sensor / data logger
 Enzyme Temperature
What was the result of your investigation?

Activity increases or enzyme has an optimum

or graph showing increase