Lipase

Contents

What is Lipase?

Where is it found?

Function

Clinical Significance & Profile

Lipase & Amylase tests

Infinite Lipase

Infinite Lipase - Features

 What is LIPASE?

A lipase is a water-soluble enzyme that

catalyzes the hydrolysis of ester bonds in
water–insoluble,
water–insoluble lipid substrates.

Lipases thus comprise a subclass of the

esterases.
 Where is it found?
Lipases are present throughout our
body.

Most of the lipase is manufactured in

the pancreas, although some of it is
also
secreted in the saliva.
 Where is it found?

Pancreas produces about litre and a

half of digestive juices per day e.g.
protease, amylase, lipase, cellulase &
lactase.

Lipase is the enzyme, which is

produced to digest fats.

LIPASE
 Sources of Lipase…
The different types of lipases found in our
body include –

– Pancreatic lipase – secreted by pancreas

– Hepatic lipase – present in Liver

– Lipoprotein Lipase/Endothelial lipase –

present in the endothelial cells

– Lysosomal lipase – present intracellularly

– Gastric lipase – secreted in the stomach

 Function

Lipases perform essential roles in

the:-

• Digestion

• Transport &

• Processing of dietary lipids in most-

if not all- living organisms. (e.g.
triglycerides, fats, oils)
 Function

Hydrolysis of triglyceride into

monoglyceride and free fatty acids is
accomplished predominantly by
pancreatic lipase.

The activity of this enzyme is to clip

the fatty acids at positions 1 and 3 of
the triglyceride, leaving two free
fatty acids and a 2-monoglyceride.
 Function

Hydrolysis of triglyceride into monoglyceride and free fatty acids

3
 Clinical Significance

Elevated levels of lipase in blood may

indicate a problem related to pancreas.

When the pancreas are inflamed,

increased blood levels of the pancreatic
enzymes, amylase and lipase will result.

The normal lipase level is 13-60 U/L. 

Normal values may vary from laboratory
to laboratory.
 Clinical Significance
• Causes of hyperlipasemia (high lipase levels)
may include:

– Pancreatitis - also known as inflammation of the

pancreas, can cause amylase and lipase levels to be
increased up to 3 times normal. Both values should be
increased, in order to carry the diagnosis of pancreatitis.

– Lipase may be increased in tumors of the pancreas,

or stomach.

– Gall bladder infection - Inflammation of the gall

bladder (cholecystitis), may cause increased lipase
levels.

– Kidney failure can cause hyperlipasemia.

 Tested in…

Related Test: Amylase

 Lipase & Amylase tests
Amylase & Lipase tests are used to
monitor the diagnosis and treatment of
acute pancreatitis and to differentiate
pancreatitis from other abdominal
disorders

Blood amylase levels are sensitive for

pancreatic disorders but are not specific.

An elevated amylase may indicate a

problem but non-pancreatic disorders can
cause increased amylase levels.
 Lipase & Amylase tests

Lipase levels are usually increased in

pancreatic disorders and are more
specific than amylase for diseases of the
pancreas.

Lipase levels remain in the blood for a

longer duration than amylase.

Evaluating the results of the two tests

together help to diagnose or rule out
pancreatitis and other conditions.
 Summary

water-soluble
What is Lipase?
enzyme
Pancreas
Where is it found?
Digestion of dietary
Function –
Lipids
Pancreatic
Clinical Significance & Profile –
disorders
Lipase is more
Lipase & Amylase tests –
specific

& remains
longer in serum
 Infinite Lipase
• Principle:

1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6-
methylresorufin) ester
Lipase/Colip
ase
1,2-o-dilauryl-rac-glycero + glutaric acid-(6-
methylresorufin) ester
Alkaline
medium
glutaric + 6-
acid methylresoruf
in
 Infinite Lipase
• Simple Assay Procedure:

R1: 800 μl + Sample: 10 μl

Mix & incubate for 5 min at

37°C
R2: 200 μl
120 Mix &
seconds aspirate
A
60 1
seconds
A
2
60
seconds
Measure at 578 A
nm. 3
 Infinite Lipase

• Colorimetric method

• Increasing Kinetic reaction

• Wavelength – 578 nm.

• Reaction temperature – 37°C

• Zero setting – Reagent Blank

 Infinite Lipase
• Delay time – 120 seconds

• Interval – 60 seconds

• No. of readings – 3

• R1 & R2 volume – 800 μl & 200 μl

• Linearity – 300 IU/L

• Calculation:
Lipase (IU/L) = Factor x ΔAbs./min of
Sample
 Infinite Lipase - Features

• Ready-to-use, two liquid reagent

system

• Colorimetric detection of Lipase

• High Linearity of 300 IU/l

• Free Calibrator provided with the kit

• Pack-Size: 25 ml
(R1: 2 x 10 ml; R2: 2 x 2.5 ml; )