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Contents
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The Chemistry of Heredity
Genetics: Fundamentals
Human Genome:
- 10 million million
(10x1012) cells with a
nucleus
- each cell has a complete
set of genetic
instructions to make
another you(biologically)
- 23 pairs of chromosomes
- 100,000 genes
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DNA
DNA (deoxyribonucleic acid):
molecule that carries genetic
information in all species
3 parts to DNA:
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DNA
Put all 3 groups together,
called a nucleotide
Adenosine phosphate
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The percenst base composition of DNA
for various species
Common
Species Adenine Thymine Guanine Cytosine
Name
Homo sapiens Human 31.0 31.5 19.1 18.4
Drosophila melanogaster Fruit fly 27.3 27.6 22.5 22.5
Zea mays Corn 25.6 25.3 24.5 24.6
Neuspora crassa Mold 23.0 23.3 27.1 26.6
Escherichia coli Bacterium 24.6 24.3 25.5 25.6
Bacillus subtillis Bacterium 28.4 29.0 21.0 21.6
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The Double Helix of DNA
X-Ray Diffraction of DNA
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DNA
Turns out that:
Called
complementary
bases:
A=T
G=C
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DNA Double Helix
DNA is double stranded and
is shaped like a spiral
staircase.
ATAGCCG
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DNA Replication
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Cracking the Chemical Code
DNA: Blueprint of Life?
How does DNA provide genetic information, where does the
information come from?
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DNA: Blueprint of Life?
How does DNA provide genetic information, where does the
information come from?
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DNA to Proteins
OK, the instructions are in the sequence of bases.
There are 20 amino acids
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DNA to Proteins
OK, the instructions are in the sequence of bases.
There are 20 amino acids
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DNA to Proteins
OK, the instructions are in the sequence of bases.
There are 20 amino acids
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DNA to Proteins
OK, the instructions are in the sequence of bases.
There are 20 amino acids
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Codon table
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DNA to Proteins
Transcription: DNA to mRNA
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Protein Structure and Activity:
Form and Function
Polypeptide
Backbone
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Protein structure
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Secondary structures
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Tertiary structure
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The Human Genome Project
What is the Human Genome Project?
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Benefits of Human Genome Project research
• improvements in medicine.
• microbial genome research for
fuel and environmental
cleanup.
• DNA forensics.
• improved agriculture and
livestock.
• better understanding of
evolution and human migration.
• more accurate risk assessment.
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Genetically Engineered
Medical Treatment
Recombinant DNA: manipulating gene pools
Cut out DNA that encodes for
human insulin
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How to create transgenic plants
Agrobacterium tumefaciens
Ti Recombinant
plasmid Insertion of gene Ti plasmid Introduction Regeneration
into plasmid using into plant of plant
restriction enzyme cells in
T DNA and DNA ligase culture
T DNA carrying new
gene within plant chromosome Plant with new trait
Restriction site
• Nitrogen fixing corn,
corn that capable in • Transgenic soybeans
fixing N2 which is resistance to
• Contain nitrogen fixing herbicides
bacterial genes
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Mixing Genes: Transgenic Organisms
Cloning Mammals and
Humans
Do you know these movies?
Jurassic Park
The Lost World
The Boys from Brazil
Sleeper.
Multiplicity
Invasion of the Body Snatchers
Stepford Wives
A Stolen Life
Clones
The Clones of Bruce Lee
Blade Runner
Star Wars.
Judge Dredd
American Ninja 2
Twins
Gattaca
Alien Resurrection
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Nuclear transfer/ Somatic cell nuclear
transfer
(A, B) nucleus is sucked of cell
(C) New nucleus is picked up
(D) Nucleus/DNA is inserted
into the egg
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Dolly, 1996-2003
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Snuppy, 2005
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Human cloning
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The New Prometheus?
PROMETHEUS
• PROMETHEUS was the Titan god of forethought and crafty
counsel who was entrusted with the task of moulding mankind
out of clay. His attempts to better the lives of his creation
brought him into direct conflict with Zeus. Firstly he tricked the
gods out of the best portion of the sacrificial feast, acquiring
the meat for the feasting of man. Then, when Zeus withheld
fire, he stole it from heaven and delivered it to mortal kind
hidden inside a fennel-stalk. As punishment for these
rebellious acts, Zeus ordered the creation of Pandora (the first
woman) as a means to deliver misfortune into the house of
man, or as a way to cheat mankind of the company of the
good spirits. Prometheus meanwhile, was arrested and bound
to a stake on Mount Kaukasos where an eagle was set to feed
upon his ever-regenerating liver (or, some say, heart).
Generations later the great hero Herakles came along and
released the old Titan from his torture.
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Prometheus
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• We have found key natural compounds of life
“DNA”
• We have clone gene and took many advantage
for human being
• We have clone animals
• Next logical step would be creating new organism
or cloning human “the super one”
• However we could not removed defective gene
from the pools
• And cloning or creating superman could end up
with creating a new prometheus
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Thank you
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