Documenti di Didattica
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Infrared
Spectroscopy)
Prepared by Tiya Novlita
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What is NIR and Vis spectroscopy?
2
Region of IR
3
Types of molecular motions
Low energy
• Translational motion
• Vibrational motion
• Rotational motion
High energy
4
NIR Interaction
Macroscopic Microscopic
Infrared Light
5
5
Take a peek on how NIR instrument works!
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Example of NIR Spectra
Combinations
0,5
0,45 Caffeine
0,4
Absorbance
0,35
0,3
0,15
0,1
2nd Overtones
0,05
0
1100 1300 1500 1700 1900 2100 2300 2500
Wavelength
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Sampling Technique
Transmission Diffuse Reflectance
• Useful for materials which are “diffuse”
• Generally used on and reflect the NIR beam back. Samples
translucent solids, liquids, typically analyzed by diffuse reflectance
include powders, solids, tablets and
gels and tablets. suspensions.
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Advantages and Disadvantages
ADVANTAGES DISADVANTAGES
• Fast – With typical • Not a Primary method
measurement times of around
30 seconds. • Needs many standards for
• No Sample preparation. making a good calibration
• No wastage and No pollution. model
• Savings in running costs (no
reagents, consumables, etc)
• Simultaneous determination
of multiple components.
• Measurement through
packaging
• Ease of operation
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Up for more debates?
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Taken from Spectroscopy : NIR
Versus Mid-IR: How to
Choose?
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