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Microscope
Slide # 2
M
B B. Arm: supports tube & connects it to
the base
L C C. Stage Clip: holds microscope slide in
place
K
D. Coarse adjustment: raises / lowers
J stage to bring image into focus
D
E. Fine adjustment: brings image into
I E sharp focus
H F. Base: Supports microscope
G F
G. Illuminator: Light source
H. Diaphragm: Controls the amount of
light that passes through a specimen
L. Revolving nosepiece: holds 2 or I. Stage: platform that holds the slide
more objective lenses J. Microscope slide: holds the specimen
M. Body tube: Connects eyepiece K. Objective lenses: magnifies the
to objective lens specimen
– Shortest lens has least
magnification
– Longest lens has greatest
magnification
Slide # 3
B
M
B
M
L. Revolving nosepiece:
L C holds 2 or more objective
K lenses
J
D M. Body tube: Connects
I E eyepiece to objective lens
H
G F
A
B
M
L C
J
D
I
E
G F
Slide # 5
10x X 4x = 40X
Slide # 6
TAKS PRACTICE
A student wants to view cells under the
compound microscope at a total magnification
of 400X. If the eyepiece is 10X, which of the
following objective lenses should be used?
A 4X How do we calculate
B 10X magnification?
C 40X
D 100X Eyepiece X Objective lens =
Total magnification
n = 40x
Slide # 7
How a Light Microscope Works
1. Use lenses to make small objects
appear larger
2. Compound light microscope: Two
lenses separated by a tube
3. Lenses magnify an object by
bending the light that passes
through the lens
4. Magnification: ability to make
things appear larger than they are
5. Resolution: fineness of detail that
can be seen in an image
Go to
Section:
Slide # 8
Microscope Safety
1. Always use 2 hands to carry a microscope; one on
the arm and one hand supporting the base
2. Only use lens tissue to clean lenses
3. When focusing, always look to the side to watch and
make sure the objective lens doesn’t hit the slide
4. Always use the lowest power (shortest) objective
lens for bringing specimen into focus
5. Bring specimen into focus by first using coarse
adjustment, then use fine adjustment
6. Never use a microscope with a frayed cord
7. Because we have running water in our lab area,
NEVER turn on the water when using a microscope
Slide # 9
How to Prepare a Slide