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PRELIMINARY INVESTIGATION
OF THROMBOLYTIC ACTIVITY OF
CRUDE METHANOLIC EXTRACT
AND SILVER NANO PARTICLE
OBTAINED FROM
DRYNARIA QUERCIFOLIA
Guided by Submitted By
Mrs.S.CHITRA,M Sc.,M.Phil., R.DEEPA
(16CAB1006)
Assistant Professor MASTER OF PHILOSOPHY IN BIOCHEMISTRY
M.G.R COLLEGE
DEPARTMENT OF BIOCHEMISTRY Dr.M.G.R Nagar,
M.G.R COLLEGE Hosur-635 109
Hosur-635 109
INTRODUCTION
Nanotechnique is the science which deals in materials in the range of 1 to 100 nm.
These nanoparticles have multiple advantages in various fields such as electronics, cosmetics, coatings,
Due to their optical properties the colloidal solution of metal nanoparticle is transparent, thus they are useful
Among nanoparticles, silver nanoparticle has wide application in industry and medicine due to its
antimicrobial, antifungal, and anti-parasitic characters. Because of their wide applications beneficial to
humans there is a need to develop rapid and reliable experimental protocols for the production of silver
nanoparticles.
The production of silver nanoparticles using biological entities is gaining momentum as; biological methods
busting.
It works by stimulating secondary fibrinolysis by plasmin through infusion of analogs of tissue plasminogen
activator (tPA), the protein that normally activates plasmin. Some commonly used thrombolytics are: Streptokinase,
Urokinase.
Formation of blood clots lies at the basis of a number of serious diseases. By breaking down the clot, the disease
process can be arrested, or the complications reduced. While other anticoagulants prevent the "growth" of a clot,
thrombolytic agents actively reduce the size of the clot (Sardar M 2005) Most thrombolytic agents work by
activating the enzyme plasminogen, which clears the cross linked fibrin mesh .
This makes the clot soluble and subject to further proteolysis by other enzymes, and restores blood flow over
Kingdom : Plantae
Division : Pteridophyta
Class : Polypodiopsida/Pterdopsida
Order : Polypodiales
(unranked) : Eupolypods I
Family : Polypodiaceae
Tribe : Drynarieae
Genus Drynaria
Species : D. quercifolia
Binomial name
Drynaria quercifolia
Drynaria quercifolia
AIM AND OBJECTIVES
To prepare crude methanolic extract of Drynaria
quercifolia plant.
To perform phytochemical analysis of the crude
methanolic extract of Drynaria quercifolia plant.
To synthesis of silver nano particles from the methanolic
extract.
To evaluate antibacterial activity and thrombolytic activity
of Drynaria quercifolia extract and silver nanoparticles.
MATERIALS AND METHODS
Selection and collection of medicinal plant
Solvent Extraction
Venous blood was drawn from healthy volunteers (n = 10) and transferred in different pre-weighed sterile alpine
tube (500 μl/tube) and incubated at 37°C for 45 minutes. After clot formation, serum was completely removed.
Each tube having clot was again weighed to determine the clot weight.
Each alpine tube containing clot was properly labeled and 100 μl of plant extract was added to the tubes. As a
positive control, 100 μl of SK and as a negative non thrombolytic control, 100 μl of distilled water were separately
added to the numbered control tubes.
All the tubes were then incubated at 37°C for 90 minutes and observed for clot lysis. After incubation, fluid
obtained was removed and tubes were again weighed to observe the difference in weight after clot disruption.
Difference obtained in weight taken before and after clot lysis was expressed as percentage of clot lysis.
1 Alkaloids +
2 Carbohydrates +
3 Saponins +
4 Glycosides +
6 Phytosterol +
7 Phenolic compounds +
8 Flavonoids +
9 Terpenoids +
10 Tannins +
ANTI BACTERIAL ACTIVITY
OF METHANOL EXTRACTS
E.coli
SEM image of silver nano particals
synthesized from D.quercifolia
Thrombolytic activity
Thrombus or embolus hinders the blood flow by blocking the blood vessel therefore depriving tissues of
normal blood flow and oxygen.
These consequence yield necrosis of the tissue in that area. Thrombin formed blood clot from fibrinogen
and is lysed by plasmin, which is activated from plasminogen by tissue plasminogen activator (tPA).
The purpose of a fibrinolytic drug is to dissolve thrombin in acutely occluded coronary arteries thereby to
restore blood supply to ischemic myocardium, to limit necrosis and to improve prognosis.
For the treatment of myocardial infarction, many thrombolytic agents are used. Among them,
streptokinase is remarkable and widely used.
Moreover, Tissue-type Plasminogen activator is more effective and safer than either urokinase or
streptokinase type activators. It is noted that all available thrombolytic agents still have significant
deficiencies, including the necessity of large doses to be maximally effective, limited fibrin specificity
and a significant associated bleeding tendency.
Therefore, steps are taken to develop improved recombinant variants of these drugs in order to minimize
deficiencies of the available thrombolytic drugs.
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Thank you