Graduate Student: Tran Tan Tien

Supervisor: Dr. Tran Thi Ngoc Dung

Nano Scale
Abstract
Introduction
Types of Nanobiosensors
Working principle of Nanotube Based Biosensors
Current Research and Applications of
Nanobiosensors
What is a Nanobiosensor?
Introduction
Basic concept - 8 major devolpment areas
Optics Smart Materials Nanoscale
Biostructure

Magnets Nanoscience &

Nanotechnology
Energy Capture,
Transformation,
Fabrication and Storage
Electronics Sensors

Ratner, Mark A., and Daniel Ratner. Nanotechnology: A gentle introduction to

the next big idea. Prentice Hall Professional, 2003.
 I.) Introduction

Classification based on Applications

Chemical Nano- Deployable

nanosensors sensors nanosensor

Biosensors Electrometers

Agrawal, S., and R. Prajapati. "Nanosensors and their Pharmaceutical Applications: A Review."
International Journal of Pharmaceutical Science and Technology 4 (2012): 1528-1535
 What is a Nanobiosensor?
A biosensor is a measurement system for the detection of an analyte that
combines a biological component with a physicochemical detector, and a
nanobiosensor is a biosensor that on the nano-scale size
Nanobiosensor

Transducer Detector Biological Recognition Element

(Bioreceptor)
Living biological system
(cell, tissue or whole organism)
Biological molecular species
antibody, enzyme, protein
Building blocks of life
 WORKING PRINCIPLE OF A
BIOSENSOR

The interaction of the analyte with the bioreceptor is designed to

produce an effect measured by the transducer, which converts the
information into a measurable effect, such as an electrical /
electronic signal.
 (f
(e) )
(d)
(a)
(c)
(b
)
The interaction of the analyte (a) with the bioreceptor, which identifies
the stimulus (b) is designed to produce an effect measured by the
transducer (c), which converts it to an electrical signal. The output from
the transducer is amplified (d), processed (e) and displayed (f).
Enzymatic Biosensors - Principles and Applications. D. Grieshaber, R. MacKenzie, J. Vrs, and E. Reimhult,
Sensors, 8(3); 1400-1458, 2008.
HISTORY- Biosensor
1962- first description of a biosensor of : an amperometric enzyme electrode (Glucose
sensor) by Clark.
1969- first potentiometric biosensor : urease immobilized on an ammonia electrode to
detect urea by Guilbault & Montalva.
1970- ion-selective Field Effect Transistor (ISFET) by Bergveld.
1975- fibre-optic sensor with immobilized indicator to measure carbon-di-oxide or
oxygen by Lubbers & Optiz.
1975- first commercial biosensor (Yellow Spring Instrumental Biosensor) .
1975- first microbe based biosensor (first Immunosensor)..
1976- first bedside artificial pancrease.
1980- first fibre-optic pH sensor for in-vivo blood gases by Peterson.
1982- first fibre-optic based biosensor for glucose.
1983- first surface plasmon resonance (SPR) immunosensor.
1984- first mediated Amperometric biosensor.
1987- Blood Glucose biosensor launched by Medi-sense Exac Tech.
1990- SPR based biosensor by Pharmacia BIA Core.
1992- hand-held blood biosensor by i-STAT..
1996- launching of Gluco-card.
1998- blood glucose biosensor launched by Life-scan Fast Take.
2000- nanotechnology biosensor, chip,quantum dots etc..
 1962 First description of a biosensor: an amperometric
enzyme electrode for glucose. [Leland Clark, New York
Academy of Sciences Symposium]
pH sensor for in-vivo blood

1980- first fibre-optic

gases by Peterson.

1987- Blood Glucose biosensor launched by Medi-

sense Exac Tech.
 2000-nanotechnology biosensor, chip,quantum dots
etc..
Classification
Nanobiosensors: The Merging of Nanotechnology with
Biosensors
Classification of nanobiosensors
Acoustic
Nanoparticle
Wave
based sensors
Biosensors

Nanobiosensors
Magnetic
Nanotube Biosensors.
based sensors

Electrochemical
Nanowire
Biosensors
Based Sensors

Malik, P., Katyal, V., Malik, V., Asatkar, A., Inwati, G., & Mukherjee, T. K.. Nanobiosensors: Concepts
and Variations. (2013) ISRN Nanomaterials, 2013.
(SWCNT)-based field-effect transistors
(FETs)
Single-wall carbon nanotubebased field-effect
transistors(FETs)

Balasubramanian, Kannan, and Marko Burghard. "Biosensors based on carbon nanotubes." Analytical and
bioanalytical chemistry 385.3 (2006): 452-468.
Cabon nanotube interaction
Interaction DNA
Balasubramanian, Kannan, and Marko Burghard. "Biosensors based on carbon nanotubes." Analytical and
bioanalytical chemistry 385.3 (2006): 452-468.
Experiment method
Nanomaterials and nanofabrication technologies
are increasingly being used to design novel biosensors.
Investigate important fundamental to develop
nanobiosensors.
Computer modeling of nanoscale
and biophysical systems
Structure of DNA-Carbon Nanotube
Hybrids

http://www.physics.upenn.edu/~robertjo/research/
Computer modeling of nanoscale
and biophysical systems

Free Energy Landscape of a DNA-Carbon Nanotube Hybrid

Recent development topics on
biosensors
Electrochemical biosensor
Fiber-optic biosensor
Carbon Nanotube biosensor
Protein Engineering for biosensors
Wireless Biosensors Networks
Applications of Nanobiosensors
Biological Applications
DNA Sensors; Genetic monitoring, disease
Immunosensors; HIV, Hepatitis,other viral diseas, drug testing,
environmental monitoring
Cell-based Sensors; functional sensors, drug testing
Point-of-care sensors; blood, urine, electrolytes, gases, steroids,
drugs, hormones, proteins, other
Bacteria Sensors; (E-coli, streptococcus, other): food industry,
medicine, environmental, other.
Enzyme sensors; diabetics, drug testing, other.
Environmental Applications
Detection of environmental pollution and toxicity
Agricultural monitoring
Ground water screening
Ocean monitoring
Reference
Ratner, Mark A., and Daniel Ratner. Nanotechnology: A gentle
introduction to the next big idea. Prentice Hall Professional, 2003.
Agrawal, S., and R. Prajapati. Nanosensors and their Pharmaceutical
Applications: A Review." International Journal of Pharmaceutical Science
and Technology 4 (2012): 1528-1535
Zhang, X.; Guo, Q.; Cui, D. Recent Advances in Nanotechnology
Applied to Biosensors. Sensors 2009, 9, 1033-1053.
Jianrong C, Yuqing M, Nongyue H, Xiaohua W, Sijiao L. Nanotechnology
and biosensors. Biotechnol Adv. 2004 Sep;22(7):505-18.
Malik, P., Katyal, V., Malik, V., Asatkar, A., Inwati, G., & Mukherjee, T. K..
Nanobiosensors: Concepts and Variations. (2013) ISRN Nanomaterials,
2013.
Balasubramanian, Kannan, and Marko Burghard. "Biosensors
based on carbon nanotubes." Analytical and bioanalytical
chemistry 385.3 (2006): 452-468.