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The genome is the entire DNA content of a cell, including all of the
genes and all of the intergenic sequence
The human genome contains ~ 8o.ooo genes but the coding
regions of these genes take up only 3% of the genome
The yeast genome contains 6000 genes and has a more compact
organization
Prokaryotic genomes are small with very little space between
genes
Understanding the information contained in genome sequences will
be the major challenge of the early 21st century.
Human Genome
Nuclear genome:
3 X 109 bp of DNA
24 linear DNA molecules (55 250 Mb) in a different chromosome.
24 chromosome : 22 autosomes and
2 sex chromosomes (X and Y)
Mitochondrial genome:
A circular DNA molecule of 16560 bp
Located in mitochondria
Sugar: 2-ribose
Thymine (T) in DNA is replaced by Uracil (U) in RNA
Rarely more than a few thousands nucleotides in
length
Usually single-stranded
Base pairs might be formed between different parts of
a single molecule
mRNA, tRNA, rRNA
GENE
DNA Replication
Gene Transcription: RNA synthesis
Genetic code or codon in mRNA
Translation: Protein synthesis
DNA Replication
Errors in replication
Effects of chemical and physical mutagens that
directly alter the chemical structure of DNA
Deletion, Insertion, Substitution
Repair process:
DNA repair enzyme (DNA polymerase)
Recombination
Photoreactivation
Human Genome Projects
To obtain the complete sequences of human genome
Genome sequences: the key to the continued
development of not only molecular biology and
genetics, but also biochemistry, cell biology and
physiology molecular life sciences.
A catalog containing a description of the sequence of
every gene in a genome
A comprehensive description of the molecular activities
of living cells and the ways in which these activities are
controlled.