Objectives

To determine the properties and quality of

the horse joint supplements Legend and
Hyalovet by:
Using the rheometers to measure the
viscosity and elasticity.
Finding the intrinsic viscosity using capillary
viscometers.
 Rheometers

Rate Control Strain Control

 Viscometer
Glass tubes that can
be used to calculate
Timing
Lines the intrinsic viscosity
of polymer solutions
by measuring the
rate at which a liquid
falls through the
capillary at different
Capillary dilutions.
 Hyaluronic Acid

Biopolyelectrolyte Sources of HA
High molecular weight rooster combs
viscoelastic properties micro-organisms
costs 100,000 per kilogram umbilical cords
 Intrinsic Viscosities of HA Supplements in
PBS
2000.00

1800.00

1600.00
Intrinsic Viscosity = 1074 ml/g

1400.00
(T-TS)/(TS*C)

Trial 1 Hyalovet
1200.00
Trial 2 Hyalovet

1000.00 Trial 1 Legend

800.00
IV = 991 ml/g Trial 2 Legend

Linear (Trial 2
Hyalovet)
600.00 Linear (Trial 1
IV = 534 Hyalovet)
IV = 531 Linear (Trial 2
400.00
Legend)
(ml/g) Linear (Trial 1
200.00 Legend)

0.00
0.00E+00 2.00E-04 4.00E-04 6.00E-04 8.00E-04 1.00E-03 1.20E-03

Concentration HA (mg/ml)
 Moduli of Legend and Hyalovet

10.00

1.00
Moduli (Pa)

Legend Elastic Modulus (Pa)

Legend Viscous Modulus (Pa)
0.10
Hyalovet Elastic Modulus (Pa)
Hyalovet Viscous Modulus (Pa)

0.01

0.00
0.10 1.00 10.00

Frequency (hz)
 Viscosity of Legend and Hyalovet

1.00
Viscosity (Pas)

Legend Viscosity
0.10
Hyalovet Viscosity

0.01
0.10 1.00 10.00 100.00 1000.00

Shear Rate (1/s)

They Came to Tame the Lame
 Conclusions
The experiments performed on the
rheometer showed similar readings for the
flow of Legend and Hyalovet
Legend and Hyalovet showed the same
viscous and elastic moduli.
The intrinsic viscosity of Hyalovet is twice
that of Legend which shows that Hyalovet
has a larger molecular weight.