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TECHNIQUES
PHARMACEUTICAL RADIOCHEMISTRY
AND NUCLEAR PHARMACY
PHARMACEUTICAL RADIOCHEMISTRY
Method of Radiation
Radioactive Isotopes
INTRODUCTION TO RADIOCHEMICAL TECHNIQUES
Radiochemical
method
depend upon
Type of Energy of
Half Life of
emitted emitted
particles
radiation radiation
HALF LIFE
It is abbreviated as t1/2
It is the time taken for the radioactivity of a specified
isotope to fall to half its initial value
Iodine-131 has half life of 8.1days
METHODS OF RADIATION
Method of
radiation
Activation
analysis/Neutron
Tracer method Isotope dilution
activation analysis
(NAA)
NEUTRON ACTIVATION ANALYSIS
Those that decay are called radioactive (or parent) isotopes; those
that are generated by decay are called radiogenic (or daughter)
isotopes.
The rate of decay of radioactive substances such as carbon 14 or
uranium is measured in terms of their half-life.
Radioactive decay involves the spontaneous transformation of one
element into another.
The only way this can happen is the change in number of protons
in the nucleus.
RADIOACTIVE DECAY
U-238
(radioactive Th-234
Pa-234
isotope of (Thorium)
uranium)
14-steps
Pb-206
Decay sequence or series stops
(stable)
RADIOACTIVE DECAY
Radioactivity corresponds
to neutron/proton ratio in
atom
The neutron/proton ratio for a certain element must fall within a certain range for the
element to be stable. Thats why some isotopes of an element are stable and other
are radioactive.
TYPES OF RADIOACTIVE DECAY
Types of
Radioactive
decay
Gamma
Alpha particle Beta particle Less common
radiation
emission emission types
emission
Positron
Emission
Electron
capture
Isomeric
transition
ALPHA EMISSION
ALPHA EMISSION
Ionization
Liquid scintillation Radiometric
method/Gas-Filled
method techniques
Detectors
Inverse isotopic
dilution analysis
IONIZATION METHOD
These include
Cutie-Pie counter
Dose calibrators
DOSE CALIBRATORS
Cylindrical shaped
Sealed chamber with a central well
Filled with argon and traces of halogen at high
pressure
Operating voltage is 150V
DOSE CALIBRATORS
Schematic
diagram of
Dose
Calibrator
DOSE CALIBRATORS
GEIGER-MULLER COUNTERS
One end of the detector is made of thin mica window that allows passage of Beta and Gamma
rays
Add-organic
Small amount of solvent
Add into solvent Solution stirred
radiotracer containing
suitable reagent
It is applied for:
Elemental analysis of geological, biological, and environmental
samples
Determination of
Vitamins
Antibiotics
Aminoacids
Hormones
Study of interaction of elements in diease
Determination of total volume of the blood in human body
DIRECT ISOTOPIC DILUTION ANALYSIS
Radiotracer is
placed in glass
Spotted with for development of
Asbestos paper chamber containing
radiotracer spot
dilute HCl and all the
paper
O-15 (CO2) is used for labeling hemoglobins and for clinical investigation of pulmonary and cardiac
malfunctions
Gr-51 is used in Cr-51 Labeled Red blood cell method to measure blood volume
P-32 (P-ortho phosphate) is used to treat bone pain due to bone metastasis
REFERENCES
The End