lant mitochondria MITOCHONDRIAL FUNCTIONS IN PLANTS Plants have 2 energy generating organelles:
chloroplasts, responsible of photosynthesis, which t
urns CO2 and sun light into glucose and oxygen.
mitochondria, which uses oxygen ang glucose to cre
ate ATP and water. Genetic coordination As mitochondrion, chloroplast, and nucleus have wi thin them their own genome, the expression of thei r genes requires coordination.
This coordination is needed not only for the produc
tion of components for respiration and photosynthe sis, but also for transcription, post-transcriptional re gulation, translation, replication, and organellar tra nsmission for maintenance. Genetic system Due to the fact that mitochondria has its own genome within itself, genetic systems are, SOMETIMES indepen dent of the nucleus as they follow the instructions in t heir own genetics, including: - uniparental inheritance - somatic recombination - vegetative segregation - gene expression - genome organization. Genetic transference Mitochondria requires a lot of nuclear genes, result of constant gene transfer with the nucleos througho ut evolution.
This transfer is thought to have happened via RNA in
termediates.
Within the plant kingdom there are examples of evol
utionary intermediates. Genetic transference Legumes prove that many functions originally encoded within th e mitochondria are now being transferred to the nucleus.
For example, Cyt oxidase subunit (coxll) gene:
Pea: present in mitochondria and nucleus, but only expresse
d in mitochondria Soy & common bean: present in mitochondria and nucleus, b ut only expressed in nucleous. Mung bean & cowpea: gene no longer present in mitochondr ia, only in nucleus, where it gets expressed. Plant mitochondrial genome Mitochondrial DNA concentration vary greatly at di fferent stages of plant development, presenting yet another form of regulation required within the cell.
Respiratory demands vary greatly among different p
lant tissues, with the highest respiratory rates occur ring during seed germination, pollen development, and fruit ripening. This variation in respiratory activi ty is reflected in altered regulatory signals at all thre e genome levels Plant mitochondrial genome The plant mitochondrial genome is different from o ther eukaryote mithocondrial genome in their high diversity.
Mitochondrial DNA recombination between homol
ogous repeated sequences as well as heterologous sequences resulted in a high variable genome. Mitochondrial DNA recombina tion Multipartite genome structure: Due to the presenc e of recombinable repeated sequences, generated by both intermolecular and intramolecular recombi nation events.
Subgenomic DNA molecules and inversions within t
he genome: Due to the presence of repeats in direc t and inverted orientation. Mitochondrial DNA recombina tion Evidence that homologous recombination events m ay occur at a high frequency in plant mitochondria c omes from the high level of sequence identity main tained among the homologous repeated sequences .
Such sequence conservation could result from copy
correction during the course of frequent DNA excha nge Structural diversity models Brassica campestris 1. Mitochondrial genome contain recombinable repeats. 2. Recombination forms a two parts structure genome from a sing le structure genome Structural diversity models Zea mays 1. Multiple recombinable repeats can happen in one genome. 2. Inter- and intra-molecular recombinations yield a highly com plex genome structure. 3. Genomes can be in different amounts. Structural diversity models 1. Recombinations between 2 different repeats generating del etions and duplications. 2. Some mitochondrial recombination products may be prefer entially maintained while others may be lost from the genome .