VIBRIO & PSEUDOMONAS

CHARACTERS VIBRIO PSEUDOMONAS

Morphology G ve curved rods G ve rods

Comma shaped Motile, non sporeformer
Motile polar flagella Non capsulated, possess
slime layer

Cultural
characteristics Strict aerobe Obligate aerobe
O2 requirement 6.4 9.6 6.6 7.0
PH 16C - 40C 6C - 42C
Temperature

Nutrient agar Moist, translucent, round Large, opaque convex

discs with bluish tinge in earthy smell musty
transmitted light metallic sheen

Mac conkey agar Late lactose fermenters Non lactose fermenters

Blood agar Hemodigestic hemolytic

 CHARACTERS VIBRIO PSEUDOMONAS
Selective media GTTA Monsurs taurocholate Nutrient broth
tellurite peptone water PH Pigmentation:
9.2 Pyocyanin blue green
TCBS phenazine
Thiosulphate+citrate+Brom Pyoverdin greenish
othymol blue+Sucrose yellow
BSA Alkaline Bile salt agar (fluorescein)
PH 8.2 Pyorubin
Cary Blair medium- buffered Pyomelanin
solution of NaCl+Sodium
thioglycollate +Di.sod
Phosphate+CaCl2 PH - 8.4
VR medium ( Venkatraman
Ramakrishnan) 20G crude
sea salt + 5G peptone in 1 L
dist water
Biochemical
reaction - --+ +--
IMViC + +
Nitrate reduction + +
Catalase & Oxidase Cholera red Indole + Arginine dihydrolase
nitrate reddish pink due to +ve
nitrosindole formation
 CHARACTERS VIBRIO
Virulence Factors Choleragen or Cholera toxin
Endotoxin
Pathogenesis Orally contaminated
food/drink
Survive gastric acidity
Multiply in alkaline content
of small intestine
Enter blood stream-
infection of gall bladder
Intestine produce
choleragen
Increase level of CAMP
( Cyclic adenosine 35;
monophosphate)
PSEUDOMONAS
Exotoxin A lethal
Elastase Skin & eye
Infection
Hemolysin
Enterotoxin
2 types infection
i) Community acquired
ii) Hospital acquired
Community acquired
) Suppurative Otitis
inflammation of
middle ear, ear
drum, pain &
purulent discharge
) Pulmonary infection
) Infantile Diarrhoea
) Shanghai fever
similar to typhoid
Hospital acquired
) Post operative
infection
 CHARACTERS VIBRIO PSEUDOMONAS

Pathogenesis Localised lesions

Result in active Generalized lesions
secretion of large vol of Iatrogenic infections
fluid to intestinal lumen

Fluid isotonic with

plasma more Na , HCO3
& less Cl

Rice water stool

Lab Diagnosis
Sample stool Stool, sputum , blood,
Staining pus
Culturing
Biochemical reaction
ABST
Immunological test agglutination
agglutination
 CHARACTERS VIBRIO PSEUDOMONAS

PROPHYLAXIS Environmental sanitation

Injection of toxin / formol
toxoid
Killed suspension
AlOH & Phosphate
adjuvant vaccine

TREATMENT Polymyxin,
Replacement of lost fluid & gentamycin,
electrolytes tobramycin,
Oral tetracycline Ciprofloxacin