Solve the hidden message beneath this code

IWOMEMASE. After solving it, read the results

vertically from left to right.
Hint: 3 columns starting from 0 (see the
example below) then put the code (letters)
across one by one starting from the top.

0 1 2
I W O
M E M
A S E
Solve the following code.
After getting the result, read
it vertically from left to right.
PTNAAUCREPRCTAOIHMEIL
Hint: 3 rows
(This is 5 points and another
1 point if you can define the
hidden message)
PROTEIN PHARMACEUTICALS
MARK EDREN DELA CRUZ
 Protein Pharmaceuticals

-Protein pharmaceuticals is a manipulated form of

protein, which is generated in various ways to produce
large quantities of proteins, modify gene sequences and
manufacture useful commercial products.
-Protein engineering is the process of developing useful
or valuable proteins. It is a young discipline, with much
research taking place into the understanding of protein
folding and recognition for protein design principles.
--Enzyme engineering is the application of modifying
an enzyme's structure (and, thus, its function) or
modifying the catalytic activity of isolated enzymes to
produce new metabolites, to allow new (catalyzed)
pathways for reactions to occur, or to convert from some
certain compounds into others (biotransformation).
 Types of Protein Pharmaceuticals

a. Protein Pharmaceuticals from blood-Our body is

composed of 60%-70% plasma and 8%-9% is protein.
Plasma contains many different proteins.

Here are some of the examples:

Factor VIII (For blood-clotting for hemphilia)
Factor IX (For blood-clotting for hemophilia
Albumin (osmotic balance-for kidney disease)
Ig IV (For treating infections)
Anti-Thrombin III (For blood-clotting)
Alpha I-PI (For Emphysema)
 Types of Protein Pharmaceuticals

b. Monoclonal antibodies-This are very specific for

certain antigens and cell types and can be used to target
foreign toxin, viruses or cancer cells. They are used for
drug delivery to specific targets and have a half-life often
greater than one week when humanized.

c. Peptide drugs-Many hormones are actually small

peptides (2-40 amino acids) and as such, peptides can be
synthesized and can be used as pharmaceuticals.
 Protein Biopharmaceuticals

-The formation of recombinant protein is carried out in

specialized vehicles known as vectors. Recombinant
technology is the process involved in the formation of
recombinant protein.
Two ways of generating Protein Biopharmaceuticals:
1. expression in isolated cells
2. expression in transgenic plants/animals
Six major methods of Recombinant process:
3. Isolation of gene of interest
4. Introduction of gene to expression vector
5. Transformation of host cells with the recombinant
expression vector
6. Growth of cells
7. Isolation & purification of protein
8. Characterization of protein product
 DNA Biopharmaceuticals

-Vaccines help the body fight infectious diseases.

Traditional vaccines may be live attenuated, where
pathogen is weakened through chemical or physical
process and whole pathogen that is completely killed
or destroyed chemically.
-A new generation of vaccines is the recombinant
DNA vaccines. DNA coding for an antigen is
incorporated into a plasmid and the resulting
recombinant plasmid is administered into the patient.
Quiz:
1.A type of Protein Pharmaceuticals
that comes from hormones and
such.
2-3. Two ways of generating Protein
Biopharmaceuticals.
4. This type of protein
pharmaceuticals have half-life often
greater than one week when
humanized.
5. Our body is composed of how
many percentage of plasma?