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Jurusan Kimia
Fakultas Sains & tekhnologi
UIN Maulana Malik Ibrahim Malang
The secondary
metabolit content
Husna, 2011
Musthofa, 2009
Specific parameters:
determine of the
compound/ component
that has specific funciont
to certain pharmacological
activity
Non-specific parameter:
determain the external
factor that can affect the
stability & safety of
consumers.
Heinrich, 2005
The usefulnes of
medicinal herbs in
Indonesia hasnt been
done officially
INTRODUCTION
Problem of Reseach
Problem 1st
Problem 2nd
Jurusan Kimia
Fakultas Sains & tekhnologi
UIN Maulana Malik Ibrahim Malang
INTRODUCTION
Foccus
INTRODUCTION
Advantage of research
THEORY
The Classification of Acalypha indica Linn.
(Hutapea, 1993; BPOM RI, 2010)
Kingdom : Plantae
Division
: Spermatophyta
Sub Division
: Angiospermae
Class : Dicotyledoneae
Ordo
: Euphorbiales
Family : Euphorbiaceae
Genus : Acalypha
Species : Acalypha indica Linn.
Trade name : Kucing-kucingan
Synonyms : A. spicata L., A. Ciliate L., A. canescana L., A. australis
L., A. canescens Wall.
THEORY
Standardization..
SPECIFICT PARAMETER
TEST
Non-SPECIFICT
PARAMETER TEST
Standardization: the process of development and agreement of
technical standards through a rigorous experiments and
observations that produces a set of characteristics herbal
remedies that are safe for consumption with a good quality
(Kunie et al., 2012).
STEPS OF
RESEARCH
sampling
Sample
Preparation
Analysis of
Data
Non-specific
parameter
test
Preparation of
extract
Specific
parameters test
Jurusan Kimia
Fakultas Sains & tekhnologi
UIN Maulana Malik Ibrahim Malang
Rendement: 4,51%
(w/w)
Jurusan Kimia
Fakultas Sains & tekhnologi
UIN Maulana Malik Ibrahim Malang
The Result of
Specific Parameters Test
No.
Parameter
Result
1.
9,548%
2.
79,612%
3.
4.
5.
(+)
(+)
68,2577%
33,59%
The Result of
non-Specific Parameters Test
No.
Parameter
Result
Rule
17,9497
5 30 %
(Voight, 1994)
1.
Water content
2.
Ash content
1,978
3.
0,998
1,0
(BPOM RI, 2006)
4.
4,46 g/Kg
10 mg/Kg
(BPOM, 2006)
Study of Islamic
Qs. Yasin (36): 35. 1
?That they may eat His fruit, and their hands have produced it, so will they not be greatful
2. Ali Imran (3) : 190 191:
Indeed, in thecreation of the heavens and the earth and the alternation of the night and the day are
signs for those of understanding. Who remember Allah while standing oe sitting or (lying) on their
side and give thought to the creation of the heavens and earth, (saying), Our Lord, You did no
create this aimlessly; exalted are You (above such a thing); then protect us from the punishment of
the Fire
Jurusan Kimia
Fakultas Sains & tekhnologi
UIN Maulana Malik Ibrahim Malang
Study of Islamic
3. Qs. Al Furqan (25): 2
He to whom belongs to the dominion of the heavens and the earth and who
has not taken a son and has not had a partner in dominion and has created each
thing and determined it with (precise) determination.
CONCLUSION
The results from the specific parameters
test of the ethyl acetate extract of
(Acalypha indica Linn.):
content of water soluble compounds
as much as 9.548% 0.527,
ethanol-soluble extract 79.62167%
1.902.
Alkaloid content with reagents
Dragendorff and Meyer showed
positive results.
Levels of total alkaloids in the extract
obtained by 68.2577% 3.648.
Compound berberine marker
positively identified at tR 15.76 and
19, 016 minutes by levels of as much
as 33.59%.
Jurusan Kimia
Fakultas Sains & tekhnologi
UIN Maulana Malik Ibrahim Malang
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ATT
HPLC Assay
UPLC Assay
Column
XTerra,C18,50 4.6mm
Particle size
4m particles
1.7m particles
Flow rate
Injection volume
20 l
10 min
1.5 min
2000
7500
0.2 g/ml
0.054l/ml
Acetonitrile:10.5ml,
water:21ml
Acetonitrile:0.53ml,
water:0.66ml
Delay volume
720 l
110 l
Column temperature
Resolution
30 C
35-40 Mpa
less
Less
65 C
103.5 Mpa
more
High
High
Low