Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
BY
Dr. DHARTI.V. LIMBACHIYA
FINAL YR PG SCHOLAR
DEPT. OF PG STUDIES IN DRAVYA GUNA
S.J.G.A.M.C KOPPAL, KARNATAKA
GUIDED BY
DR.RAGHURAMA SHENOY
DR.SHIVANAND .B. KARIGAR
DR.S.S.SHIRURMATH
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INTRODUCTION
In most developing countries, malnutrition and unsanitary
living system contribute to massive burden of infectious
diseases.
Despite improved living conditions, wide spread vaccination
and availability of effective antibiotics, infectious diseases
continue to take very high rank as a cause of death in the
world.
Since the discovery of these antibiotics and their uses as
chemotherapeutic agents there was a belief in the medical
fraternity that this would lead to the eventual eradication of
infectious diseases.
Risk of many adverse reactions and development of antibiotic
resistant strains of microorganisms is a burning problem.
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Groups
Study
GROUP I
Group II
NO
1.
Staphylococcus
Aureus
2.
Staphylococcus
3.
Aureus
Staphylococcus
Group
Ciprofloxacin
Kiratatikta (Swertia chirata
Aureus
III
Bush-Ham.)Kashaya
STUDY PROPER
Preparation of the media:
Sabouraud Dextrose Agar media: Final Ph was
adjusted to 5.60.2.
Preparation method:
Suspend 32.5 gm in 500ml distilled water,
heat to boiling to dissolve the medium
completely.
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Incubation:
After an hour of pre-incubation, the plates
were transferred to the incubator. It was
incubated at 370 C for 24 hours.
Recording the results:
The zones of inhibition were measured after
24 hours incubation at 37 oC
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OBSERVATION
ORGANISM
Control
Decoction
Ciprofloxacin
00.00
20.6
14.2
Staphylococ
cus aureus
00.00
24.5
11.6
Staphylococ
cus aureus
00.00
18.7
16.4
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Organism
Control
Decoction
Ciprofloxa
cin
Staphylococ
cus aureus
00.000.00
21.272.95
14.072.40
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S.AUREUS
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20
15
Mean zone of inhibition
in mm
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Observation 1
Observation 2
Observation 3
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DISCUSSION
Infectious diseases : An alarming mortality rate.
50% of mortality in developing countries.
20 % of mortality in developed countries.
Treatment of krimi roga through Ayurvedic principals - A
large number of drugs are attributed with krimighna activity
by various Samhitas and Nighantus.
The present day : Need to revalidate the treasure of
Krimighna dravyas .
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Bhavaprakash Nighantu
Krimighna
Kaiyadeva Nighantu
Krimighna
Priya Nighantu
Krimighna
Shodhala Nighantu
Krimighna
Nighantu Adarsha
Krimighna
Guna ratnamala
Krimipranut
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Disease review
Krimi : Correlated to diseases caused by micro
and macro organisms.
Sukshma (Adrusta) Krimi: Compared to the
micro organisms like bacteria, viruses, fungi, etc.
Selection of microbes
For the study gram positive bacteria was selected.
Staphylococcus aureas
The most pathogenic and mostly causes skin infections.
The most ubiquitous and dangerous human pathogens,
for both its virulence and its ability to develop
antibiotic resistance.
Pathogenic staphylococci are ubiquitos. They are
carried, usually transiently, in the anterior nares of
about 30% of healthy adults and on the skin of about
20%. Rates are higher in hospital patients and
personnel.
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Selection of decoction
Kiratatikta mainly used as Kashaya in Ayurvedic
classics.
For analyzing Ayurveda drug in Ayurvedic dosage
form, Kiratatikta Kashaya is selected for the study.
The reason behind in choosing Kashaya was to
find out whether any phyto constituent present are
heat stable that would bring antibacterial activity.
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PRAKRUTI VIGHATA.
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Katu Vipaka
KRIMIGHNA.
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Kiratatikta
Sara guna
Virechana.
Apkarshana of Krimi
Krimis inside the body: Take off the essential
nutrients from the host and cause hazards.
Bheshaja Apakarshana : Help in removal
(expulsion) of the Krimis.
Vanish the internal environment which is actually
favorable for the growth (development) of the
Krimi.
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CHEMICAL CONSTITUENTS
CONCLUSION
A phytochemical study reveals presence of
Sterols, Flavonoids, Triterpenoids, Tannin
compounds in Kiratatikta.
It is observed from the in vitro study that the
drug Kiratatikta decoction has shown
comparative significant antibacterial activity
with that of standard drug Ciprofloxacin.
It is suggested that this medicinal plant may be
used to discover new therapy to fight against
microbial infection mediated disorders.
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THANK YOU
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