Vasilios Aris Morikis

Dan DeLahunta
Dr. Hyle Park, Ph.D.

Optical Coherence Tomography

An Overview of OCT

System Setup
Sample Arm
Galvanometer

Project Overview
Methodology
Results
Conclusions

High resolution sub-surface imaging

Non-invasive

Not harmful to subject

Potential in many fields

Ophthalmology (RNFL thickness, AMD)
Dermatology (photoaging, BCC detection)
Cardiology (assessment of vulnerable
plaques)
Gastroenterology (Barretts esophagus)

Time delay between reflected light is measured to determine

depth of the reflecting structure
Due to the short time delays between signals OCT must use an
interferometer to detect the reflected light.

Interference fringes are formed when the sample and reference

arms are within a small range.
A depth profile is formed by the detection of the interference
pattern between the reference and sample arm as the reference
arm is scanned.

The intensity of the depth

profile is encoded on a
logarithmic scale.
A 2D cross section or even
a 3D volume can made by
scanning the beam across
the sample.

Helped to
construct the
Sample Arm.

Built the box to

power and
control the Galvo

Video of the
Galvo moving

Develop analysis/processing code in

MATLAB
Objective: Mathematically focus raw
data obtained from the 1310
nanometer system.

Adjust the incident angle, focal length, and

the wavelength.
Increase the signal to noise ratio (SNR) to
produce high resolution image.

Read the
Image

Flip Matrix
(if
necessary)

Interpolate

Zero Padding

FFT

Display
Image

Raw data obtained

when the reference
and sample arm are
600 microns apart.
Image taken of the
mirror.

Intensity

Pixel Number

Creates a blurred black line

when the actual image is
formed.

Completely unprocessed data.

To create accurate image
point spread function should
be narrow and high (ignore all
the noise in the middle).

Splits the matrix and adds many 0s in

Fourier space.
Doubles the size of the original graph.

Intensity

Intensity

Used to increase the point density to interpolate

more accurately.

Pixel Number

Pixel Number

Used to find remap the data linearly in

wave number (k) to improve the results
of a subsequent FFT
Takes the Intensity vs. Pixel number
graph and Intensity vs. k.

Fourier transform switches one complex

valued function into another.
Transforming k (wave number) into actual
space.

Side Camera

Straight Camera

Incident Angle

49 degrees

51 degrees

Grating Spacing

1.0e-3/1145

1.0e-3/1145

Focal Length

92 millimeters

95 millimeters

Wavelength

1350 nanometers

1350 nanometers

Pixel Width

25 micrometers

25 micrometers

Now that the parameters are correct a

much more focused image is created.
Dark line at the top is thin and not blurry

Image obtained form the

1310 nanometer system
before processing (left)
and after processing
(right).
Image width:100 microns
Image height: 500 microns

Very first image acquired

with either system.

I would like to thank NSF and the UC

Riverside BRITE program for funding, as
well as the University of California,
Riverside and NIH (R00 EB007241), and
Dr. Hyle Park and the rest of the Park
Research Group for their guidance.
Mr. Jun Wang for organizing and Dr. Victor
Rodgers for directing the program.