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Documenti di Professioni
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CONTENTS
Introduction
Classification
Types of artefacts
Remedies
Summary
References
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INTRODUCTION
ARTEFACTS
2 principle circumstances
artefacts
I)Handling of the tissue at the time of biopsy or
during fixation process
II) Histo technical procedures used for embedding
and staining the tissue
ARTEFACTS
ARTEFACTS
ARTEFACTS
Heat artefact
Caused by laser generated heat
Heated metal instrument
Over heated molten wax,
Heating on hot plate
Crush artefact
ARTEFACTS
11
Intra-lesional injection
of anesthetic solution
causing vacuolization
13
14
15
Tatoo artefact
Starch contamination
16
Cellulose artefact
17
Specimen containers
Rust
Specimen marking dyes
Foam sponges
Freezing artefacts
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Specimen
marking dye
19
Freezing artefacts
20
21
Curling artefact
Formalin haem pigment
Formalin pigment
Improper fixation
22
Curling artefact
Streaming
artefact
23
Formalin-Haeme
pigment artefact
Improperly fixed
tissue
24
Improperly fixed
tissue
Autolysis and
putrefaction
Fixation in water or
saline
25
Formalin pigment
26
27
Poor Processing
Loss of soluble
structures
28
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Coarse chatter
31
Venetian blind
Knife lines
Displacement of
components
32
Bubbles under
section
Tidemark due to
adhesive
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Dirt
Folds
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35
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Contaminated
staining solution
Residual
wax
37
Mucosal
contamination
Water
contamination
Drying of the
section
38
39
Fungal
contamination
Cellulose fibers
Presence of
hair
40
Sneezing artefact
41
42
Mountant
breakdown artefact
43
Bleaching artefact
44
45
If possible are to be
avoided
Improper surgical
removal
Pemphigus vulgaris
longer, broader,
shallower biopsy
specimen is required
Verrucous carcinoma
take a narrow deep
specimen
epithelium should be
thick to see for the
dysplastic changes if
deep seated.
Split artefact
Fragmentation
artefact
Scalpel blades
splitting of both
superficial and at the
edge of the tissue
46
During traction by the
Pseudocyst formation
Numerous spaces
lined with surface
epithelium false
impression of cysts in
connective tissue
Teeth of the
instrument force
epithelium through
the connective tissue
Heat artefacts/
fulgration
Amorphous
appearance to the
epithelium and the
connective tissue
Epithelial cells appear
detached, fusiform,
nuclei show
hyperchromatism,
spindled, palisading
configuration
Connective tissue and
muscle appear
opaque and
amorphous
Coagulation of the
tissue protein
Avoided by making
the incision margin far
away from the
interface of the lesion
with the normal tissue
Prevent accidental
contact of the cutting
tip with the metal
instruments to hold
the specimen
Initial incision with
scalpel
47
Injection artefact
Haemorrhage with
extravasation mask
the normal cellular
architecture
Seperation of
connective tissue
bands with
vacuolization
Injection of anesthetic
solution directly into
the lesion
Injection deep to the
lesion or immediately
after performing biopsy
is effective for
haemostasis
Forceps/ crush/
squeeze artefact
Grasping with
excessive force teeth of
the instrument
penetrate the tissue
Adequate retraction
and visualization
minimal force in
grasping
Use of small Adsons
forceps with or without
teeth l
48
Lesion proper should
Starch artefact
Starch granules
resemble epithelial
cells
Refractile, glassy,
polygonal, PAS +ve
cells or spore like
structures
A central dark area
pyknotic nucleus or a
mitotic figure
Light blue in H & E
Blue black in Lugols
solution
Deep iliac red with PAS
Resistant to diastase
Specimen containers
Crush/ tear/
compression
Freezing artefact
Interstitial vacuoles
49
Temp 12.2F or -11C
Fixation
Shrinkage
Curling
Brittleness
Pigmentation
Foam sponges
Triangular artefacts
Misinterpreted as
vascular spaces
Processing
a) Dehydration
Over dehydration
brittle
Interfere with staining
properties
Under dehydration
hard to section
tearing artefacts and
holes in the sections
50
Embedding
Inadequate infiltration
insufficient time in
molten wax
Vibration of the tissue
in the wax block coz of
air entrapment
Vacuum oven used
Eliminated by soaking
in the block in moist
cloth
Microtomy
Cracks, streaks,
compresion artefacts
Squamous cell
contamination highly
eosinophilic and have
large nucleus
resembling skin
epidermal cells
Scratches appear as
straight slashes or
ragged tears
51
Incomplete staining
Precipitation
Contaminated taining
solution
Mucous contamination
Inadequately filled
staining dish
Filtering or freshly
prepared stains
Using slide staining jars
Washing of the slide
Preservation artefact
Water in section
Mountant break down
Minute bubbles
entrapped in the nuclei
Leaching of the stains
Crazing or cracking
crystallization
Bleached area
52
Artefact Decalcifications
53
Incomplete
decalcification artefact
Over decalcification
artefact
54
Limitations of paraffin
wax embedding for dense
bone artefact
55
Artefact cytology
Propellent contamination
56
Cardboard imprint
Drying
57
THANK YOU
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