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Standardizing Acids
Acids are frequently standardized against weighed
quantities of sodium carbonate.
Tris-(hydroxymethyl)aminomethane, (HOCH2)3CNH2,
known also as TRIS or THAM, is available in
primary-standard purity from commercial sources. It
possesses the advantage of a substantially greater mass
per mole of protons consumed (121.1) than sodium
carbonate (53.0).
(HOCH2)3CNH2 + H3O+
(HOCH2)3CNH3+ + H2O
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APPLICATIONS OF NEUTRALIZATION
TITRATIONS
Neutralization titrations are used to determine the
innumerable inorganic, organic, and biological
species that posses inherent acidic or basic
properties. Equally important, however, are the
many applications that involve conversion of an
analyte to an acid or base by suitable chemical
treatment, followed by titration with a standard
strong base or acid.
Elemental Analysis
Several important elements that occur in organic
and biological systems are conveniently
determined by methods that involve an acid/base
titration as the final step. Generally, the elements
susceptible to this type of analysis are
nonmetallic and include carbon, nitrogen,
chlorine, bromine, and fluorine. Pretreatment
converts the element to an inorganic acid or base
that is then titrated.
Nitrogen
Nitrogen occurs in a wide variety of substances of interest
in research, industry, and agriculture. Examples include
amino acids, proteins, synthetic drugs, fertilizers,
explosives, soils, potable water supplies, and dyes. Thus,
analytical methods for the determination of nitrogen,
particularly in organic substance, are of singular
importance.
The most common method for determining organic
nitrogen is the Kjeldahl mehod, which is based on a
neutralization titration. In the Kjeldahl method, the sample
is decomposed in hot, concentrated sulfuric acid to convert
the bound nitrogen to ammonium ion. The resulting
solution is then cooled, diluted, and made basic. The
liberated ammonia is distilled, collected in an acidic
solution, and determined by a neutralization titration.
Sulfur
Sulfur in organic and biological materials is
conveniently determined by burning the sample in
a stream of oxygen. The sulfur dioxide (as well as
the sulfur trioxide) formed during the oxidation is
collected by distillation into a dilute solution of
hydrogen peroxide.
S(s) + O2(g)
SO2(g)
SO2(g) + H2O2 H2SO4
The sulfuric acid is then titrated with standard
base.