Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
TEACHING OBJECTIVES
To describe the basis of cellular defense
To describe the nature of antigen-antibody reactions
To compare and contrast antibody affinity and avidity
To delineate the basis for antibody specificity and cross reactivity
To discuss the principles of commonly used tests for antigen/antibody
reactions
Types of Immuity
Non-specific
(innate) Immunity
Specific
Bodys
(acquired) Immunity
Non-Specific Immunity
Mediated
Mediated
by host cells
by host proteins
Complement system
Interferons
Non-specific Immunity-Phagocytosis
Phagocytosis Def: Ingestion of infecting
microbes by phagocytic white blood cells
(i.e., leukocytes)
Neutrophils short-lived; 60-70% of
leukocytes
Macrophages long-lived; develop from
monocytes
Phagocytosis
Non-phagocytic Cells
Non-phagocytic Cells
Natural
Interferons
- any agent
capable of eliciting an
immune response
Isolated
molecules
Molecules on surface of
cell or virus
A specific
antibody
molecule will be able to
recognize a specific
epitope of an antigen
Antibody
binds to antigen
Antibody Structure
There
Antibody Structure
NATURE OF ANTIGEN-ANTIBODY
REACTIONS
A.
NATURE OF ANTIGEN-ANTIBODY
REACTIONS
B.
NATURE OF ANTIGEN-ANTIBODY
REACTIONS
Antibody Structure
NATURE OF ANTIGEN-ANTIBODY
REACTIONS
Antibody Structure
Treating
NATURE OF ANTIGEN-ANTIBODY
REACTIONS
Antibody Structure
The
NATURE OF ANTIGEN-ANTIBODY
REACTIONS
Figure 2
NATURE OF ANTIGEN-ANTIBODY
REACTIONS
NATURE OF ANTIGEN-ANTIBODY
REACTIONS
Figure 3
NATURE OF ANTIGEN-ANTIBODY
REACTIONS
The
NATURE OF ANTIGEN-ANTIBODY
REACTIONS
AFFINITY AND AVIDITY
A. Affinity - Antibody affinity is the strength of
the reaction between a single antigenic
determinant and a single combining site on the
antibody. It is the sum of the attractive and
repulsive forces operating between the antigenic
determinant and the combining site of the
antibody as illustrated in Figure 4.
NATURE OF ANTIGEN-ANTIBODY
REACTIONS
NATURE OF ANTIGEN-ANTIBODY
REACTIONS
B.
NATURE OF ANTIGEN-ANTIBODY
REACTIONS
NATURE OF ANTIGEN-ANTIBODY
REACTIONS
SPECIFICITY AND CROSS REACTIVITY
NATURE OF ANTIGEN-ANTIBODY
REACTIONS
NATURE OF ANTIGEN-ANTIBODY
REACTIONS
3. Ag:Ab ratio - The ratio between the antigen and antibody influences the
detection of Ag/Ab complexes because the sizes of the complexes formed
is related to the concentration of the antigen and antibody. This is depicted
in Figure 6.
4. Physical form of the antigen - The physical form of the antigen influences
how one detects its reaction with an antibody. If the antigen is a particulate,
one generally looks for agglutination of the antigen by the antibody. If the
antigen is soluble one generally looks for the precipitation of the antigen
after the production of large insoluble Ag/Ab complexes. Figure 7
The type of data that is obtained from the flow cytometer is shown
below.
In a one parameter histogram, increasing amount of fluorescence
(e.g. green fluorescence) is plotted on the x axis and the number of
cells exhibiting that amount of fluorescence is plotted on the y axis.
Immunology
Information
websites:
http://pathmicro.med.sc.edu/mayer/ab-ag-rx.htm
http://www.biology.arizona.edu/IMMUNOLOGY/tutorials/an
tibody/structure.html
http://www.hhmi.org/biointeractive/vlabs/immunology/ind
ex.html
(this last site is a virtual ELISA activity)