CENTRIFUGAL ANALYZER

Group 3
MT3C
Ayson
De Ala
Elica
Marilag
Moral

CENTRIFUGAL ANALYSIS
-A discrete system where the transfer of
solutions is carried out by the use of
centrifugal force.
1967 - Dr. Norman Anderson developed a
prototype at the Oak Ridge National
Laboratory
1970 - First commercial centrifugal
analyzer was introduced as a spin-off
technology from NASA

Principle
Centrifugal forces are applied to
discretely add reagents to samples
and to propel the mixtures into the
corresponding cuvettes
Uses the force generated by
centrifugation to transfer and the
contain liquids in separate cuvettes
for measurement
Samples and reagents are added in a
specially designed centrifugal type
cuvette that has three main

Advantages
Batch Analysis
Simultaneous addition of
reagents to all samples
Requires small sample
Uses small reagent volumes
Can be programmed to carry out
many different assay methods.

Disadvantages
Only one test type can be
performed each time.
The test done per batch is
only for a specific analyte.*
The quality of cuvette and
uniformity of detection window
is crucial.

TECHNICAL PARTS

ANALYTICAL MODULE
Teflon transfer disc
Cuvette rotor
Stationary photometric, spectrophotometric
and in some instruments fluorometric
system
Synchronisation system
Device for the control and the
measurement of the reaction temperature
Washing and drying part

DETECTION UNIT
DETECTOR
MIXING CHAMBER
SYRINGE DRIVE
SIGNAL PROCESSOR
MONOCHROMATOR

How does it work?

1. Rotor disc is loaded with reagents, diluents
and samples in appropriate wells, including
the blank and control sera.
2. The rotor starts spinning and the reagents
are transferred outward through the
capillary passages due to the centrifugal
force.
3. The reaction proceeds in the peripheral
cuvettes and is detected by the
photometer, reading each cuvette several
times as the disc rotates.
4. The signals generated by each cuvette is
read and processed by a computer and the
results are displayed.

Cobas-Bio
The Cobas Bio (Roche) is a
discrete spectrometric
centrifugal analyser which is
capable of monitoring 29
reaction cuvettes. The
dispensing and analysing
systems are contained within
one unit and an Intel 8080A
microprocessor controls the
total system.

COBAS-MIRA Plus
The Roche Cobas Mira Plus CC is a benchtop random
access analyzer is designed to meet laboratory needs under
virtually any circumstances. This machine uses the same
advanced technology as the Roche Cobas Mira Plus, however,
the automated cuvette changer (CC), enables lab operators to
load up to 312 cuvettes at a time and let the analyzer run
without intervention.
This fully programmable analyzer provides trouble-free
operation, random-access testing flexibility and a broad test
menu, including STAT capability.

Key Features

All consumables available from AS Diagnostics

104 programmed tests

Photometric colorimetric optical system

End point assay method

Kinetic assay method

Direct Sampling capability

COBAS-FARA

References:
Bishop, M. et.al.; Clinical Chemistry:
Principles, Techniques and
Correlations
Vonderschmitt, D.J.; Laboratory
Organization: Automation
Ochei et.al.; Medical Laboratory
Science: Theory and Practice
http://www.ncbi.nlm.nih.gov/pmc/art
icles/PMC2547363/pdf/JAMMC-04-021.p
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