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Antigen antibodi 3

Dr.dr. I Wayan Putu Sutirta


Yasa, M.Si

Gambar Berbagai Antigen dan Epitop


I Wayan Putu Sutirta Yasa

Repeating Antigenic Determinants


e.g. PEPTIDOGYCAN

Surface
of an
Antigen
i.e.
bacterial
cell surface

Movement
at the
Hinge
Region

IgG

CH2

CH3

CH2

CH3

Gambar Konyugat Hapten-Karier merupakan Imunogen dan


Hapten merupakan Antigen yang tidak selalu imunogen
I Wayan Putu Sutirta Yasa

Epitopes: Antigen Regions that Interact


with Antibodies

I Wayan Putu Sutirta Yasa

Antibodies are Proteins that Recognize Specific Antigens

I Wayan Putu Sutirta Yasa

Gambar Ikatan silang (cross-linkage) antara TCR dan molekul MHC-II


I Wayan Putu Sutirta Yasa

Clonal Selection of B Cells is Caused by


Antigenic Stimulation

I Wayan Putu Sutirta Yasa

I Wayan Putu Sutirta Yasa

Gambar Perkembangan kadaar Ig serum pada manusia


I Wayan Putu Sutirta Yasa

Gambar Respons Antibodi Primer dan Sekunder

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I Wayan Putu Sutirta Yasa

Gambar Valensi dn Aviditas interaksi antigen-antibodi


I Wayan Putu Sutirta Yasa

Gambar Pembentukan kompelks imun dan presipitasi

I Wayan Putu Sutirta Yasa

Fungsi Antibodi

I Wayan Putu Sutirta Yasa

Laboratory on immunoassay
There are two major type measured :
Non Specific measured :
hematology,
biochemistry,
urinary test
Specific measured : serology test may be
developed a number of its own
techniques, particularly those based on
the antigen-antibody interaction

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Access is an
Immunoassay System
Chemical (Clinical Chemistry)
10-12

10-9

10-6

pg/mL

ng/mL

g/mL

10-3
mg/mL

10-0
g/mL

Immunological (Immunoassay)
Therapeutic Drugs
Thyroid Hormones
Fertility Hormones
Cardiac Markers
Cancer Markers
Infectious Disease
Vitamins
Allergy Markers
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Basic concepts

Based on : Ab + Ag = Ab:Ag (are not visualized)


Ab:Ag (in proportions at or near equivalence, can be
seen by colour (enzyme, fluorecence, protein stain);
solid phase (ery, bentonite, latex); X ray (:
detect by machinary counter), as marker.
In vivo similar to in vitro
Van der Waals Energy : specificity of Ag & Ab
Columbic Energy : electricity
Distance between antigen and antibody
pH, hydrogen, hydrophobic, electrostatic, etc

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Immunoassay methods

Divided three major methods:


Precipitation methods : high concentration, in
solution: immunodiffusion; radial immunodiffusion,
immunoelectrophoresis
Agglutination methods
: Ag / Immunoglobulin on
solid phased : slide / tube ; agglutination inhibition;
incomplete antibody tests ; complement fixation
Amplification methods : low concentration
( methods ; ELISA, RIA, PCR, IMUNOFLURESENSI)
immunofluorescense ; ELISA ; EIA; radioimmunoassay; flow
cytometry

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SIH
TERIMA KA

I Wayan Putu Sutirta Yasa

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