WARM-UP #7

DNA
(Deoxyribonucleic Acid)
Genetic material of cells
GENES units of genetic material that
CODES FOR A SPECIFIC TRAIT

Called NUCLEIC ACIDS

DNA is made up of repeating molecules
called NUCLEOTIDES


DNA Nucleotide

O
O=P-O
O
Phosphate
Group
N
Nitrogenous base
(A, G, C, or T)
CH2
O
C
1
C
4
C
3
C
2
5
Sugar
(deoxyribose)
A HISTORY OF DNA
Discovery of the DNA double helix

A. Frederick Griffith Discovers that a factor
in diseased bacteria can transform harmless
bacteria into deadly bacteria (1928)
B. Rosalind Franklin - X-ray photo of DNA.
(1952)
C. Watson and Crick - described the
DNA molecule from Franklins X-ray.
(1953)
SEE p. 292-293
Watson & Crick proposed


DNA had specific pairing between the
nitrogen bases:
ADENINE THYMINE
CYTOSINE - GUANINE

DNA was made of 2 long stands of
nucleotides arranged in a specific
way called the Complementary Rule
DNA Double Helix

Nitrogenous
Base (A,T,G or C)
Rungs of ladder
Legs of ladder
Phosphate &
Sugar Backbone
DNA Double Helix

P
P
P
O
O
O
1
2
3
4
5
5
3
3
5
P
P
P
O
O
O
1
2
3
4
5
5
3
5
3
G
C
T A
Nitrogenous Bases
PURINES
1. Adenine (A)

2. Guanine (G)

PYRIMIDINES
3. Thymine (T)

4. Cytosine (C) T or C
A or G
Chargaffs Rule
Adenine must pair with Thymine

Guanine must pair with Cytosine

Their amounts in a given DNA molecule will be
about the same.
G
C
T A
BASE-PAIRINGS

C G
H-bonds
T A
Genetic Diversity
Different
arrangements of
NUCLEOTIDES in a
nucleic acid (DNA)
provides the key to
DIVERSITY among
living organisms.
The Code of Life
The code of the chromosome is the
SPECIFIC ORDER that bases occur.

A T C G T A T G C G G
DNA is wrapped tightly around
histones and coiled tightly to form
chromosomes
See p. 297
DNA Replication

DNA must be copied

The DNA molecule produces 2
IDENTICAL new complementary
strands following the rules of
base pairing:
A-T, G-C
Each strand of the
original DNA serves as
a template for the new
strand See p. 298
DNA Replication
Semiconservative
Model:
1. Watson and Crick
showed: the two strands
of the parental molecule
separate, and each
functions as a template
for synthesis of a new
complementary strand.
.
Parental DNA
DNA Template
New DNA
1. Why is replication necessary?

2. When does replication occur?

3. Describe how replication works.

4. Use the complementary rule to
create the complementary
strand:




A---?
G---?
C---?
T---?
A---?
G---?
A---?
G---?
C---?
A---?
G---?
T---?
Replication Quiz
1. Why is replication necessary?
So both new cells will have the correct
DNA
2. When does replication occur?
During interphase (S phase).
3. Describe how replication works.
Enzymes unzip DNA and complementary
nucleotides join each original strand.
4. Use the complementary rule to
create the complementary strand:




A---T
G---C
C---G
T---A
A---T
G---C
A---T
G---C
C---G
A---T
G---C
T---A
Replication Quiz
(1961) Watson & Crick proposed
DNA controlled cell function by
serving as a template for PROTEIN
structure.

3 Nucleotides = a triplet or CODON
(which code for a specific AMINO ACID)
See p.303

AMINO ACIDS are the building blocks
of proteins.
DNA Transcription
DNA can unzip
itself and RNA
nucleotides match
up to the DNA
strand.

Both DNA & RNA
are formed from
NUCLEOTIDES and
are called NUCLEIC
acids.
See p.301
DNA Translation
The cell uses
information from
messenger RNA
to produce proteins
See p.304-305
We will
discuss details
of this on a
later date
Transcription/Translation Quiz
1. Why is transcription necessary?
2. Describe transcription.
3. Why is translation necessary?
4. Describe translation.
5. What are the main differences
between DNA and RNA.
6. Using the chart on page 303,
identify the amino acids coded for by
these codons:
UGGCAGUGC

1. Why is transcription necessary?
Transcription makes messenger RNA (MRNA)
to carry the code for proteins out of the
nucleus to the ribosomes in the cytoplasm.
2. Describe transcription.
RNA polymerase binds to DNA, separates the
strands, then uses one strand as a template to
assemble MRNA.
3. Why is translation necessary?
Translation assures that the right amino acids
are joined together by peptides to form the
correct protein.

4. Describe translation.
The cell uses information from MRNA to
produce proteins.
5. What are the main differences between
DNA and RNA.
DNA has deoxyribose, RNA has ribose;
DNA has 2 strands, RNA has one strand;
DNA has thymine, RNA has uracil.
6. Using the chart on page 303, identify the
amino acids coded for by these codons:
UGGCAGUGC
tryptophan-glutamine-cysteine

AMAZING DNA FACTS
DNA from a single human
cell extends in a single
thread for almost 2 meters
long!!!

It contains information
equal to some 600,000
printed pages of 500 words
each!!!
(a library of about 1,000 books)
LETS REVIEW DNA
LM p.44
1. List the conclusions Griffith & Avery,
Hershey & Chase drew from their
experiments.
2. Summarize the relationship between
genes & DNA.
3. Describe the overall structure of the
DNA molecule.
4. What are the 4 kinds of bases?