Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
- You should think of “safety” while doing any practical work to avoid
harming yourself or others.
Radiation hazard
Toxic
Laser Hazard Flammable- oxidizing
US protectors
Low temperature
Emergency eye wash Explosive
Exposure Routes to Harmful substances
1- Skin Absorption
Some materials are
2- Inhalation
capable of penetrating
intact, healthy skin as Of gases, vapours, aerosols,
phenol fumes
4- Injection
3- Ingestion
Accidental on contaminated food, drink
Biological Hazard
- The most obvious risks when handling microbial cultures are those due to
ingestion or entry via a cut in the skin.
BSL-1 labs: used to study agents not known to consistently cause disease in healthy
adults.
- Basic safety procedures - no special equipment or design features.
BSL-2 labs: used to study moderate-risk agents that pose a danger if accidentally
inhaled, swallowed or exposed to the skin.
- Safety measures: gloves - eyewear - hand washing sinks - waste decontamination
facilities.
BSL-3 labs: used to study agents that can be transmitted through the air and cause
potentially lethal infection.
- Safety measures: Gas-tight enclosure - specialized ventilation system - clothing
decontamination - sealed windows.
BSL-4 labs: used to study agents that pose a high risk of life-threatening disease for
which no vaccine or therapy is available.
- Safety measures: wearing full-body, air-supplied suits - shower when exiting the
facility - occupy safe, isolated zone within the building.
Substance Hazard Safety
- The ready prepared ethidium bromide solutions decrease the risk associated
with handling the solid when preparing the stock solutions.
- Inexpensive, but the hidden costs of disposal are very high. charcoal
filters, operator time to filter the waste running buffer, and bagging of solid
waste for incineration.
Disposal of ethidium bromide:
- Aqueous solutions of ethidium bromide (>1.0µ g/ml) may be disposed of in
one of two ways:
1- Adsorbed onto an ‘ion exchange column’ specifically for this purpose, These
columns will adsorb at least 300mg of ethidium bromide; the waste liquid may
then be discarded to drain. Then, the columns should be double-bagged in
plastic bags and disposed of as for solid waste.
Solid ethidium bromide waste (e.g. gels, contaminated paper towels, etc)
should be placed into a suitable, leak-tight container and then into a yellow bag
and treated as clinical waste for incineration.
SYBR Safe Green
Books:
- Reviewed in Practical skills in biology, 2003