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A. Antibiotics & antibacterial agent a. Antibiotics: Produced only by microorgnisms (Natural) b. Antibacterials: Produced: natural, synthetics or semi-synthetics B. Bacteriostatic agents & Bactericidal agents C. Narrow-spectrum & Broad-spectrum antibacterial agents D. MIC & MBC
2. Antibacterial Agents
a. Able to penetrate the bacterial surface & reach target of its action. b. Can reach the infected tissue c. Can not diffuse in to mammalian cells
3. Host
a. Intact immune system
b. vascularization & drainage
D. Protein synthesis
1. Inhibitors of the 30S ribosomal unit 2. Inhibitors of the 50S ribosomal unit
2. Other inhibitors
Inhibition of peptidoglican synthesis: - Cyclocerine - Vancomycin - Bacitracin
Penicillins
Classification
a. Natural penicillins
Penicillin G c. Extended-spectrum
penicillins (Broad-s)
a. Aminopenicillin: ampicillin, amoxycillin b. Carboxypenicillin: carbenicillin, ticarcillin c. Ureidopenicillins: azlocicllin d. Piperazine penicillin: piperacillin
b. Semisynthetics penicillins
Methicillin, oxacillin, cloxacillin, nafcillin, discloxacillin
Penicillins
1. Mechanism of action
Target:penicillin-binding proteins transpeptidase, carboxypeptidase, autolytic enzymes Inactivation of than enzymes rapid dest-ruction of peptidoglycan & dissulution cell wall bacterial lysis
3.
Spectrum
a. Natural Penicillin Narrow : Gr + & anaerob b. Semi-synthetics -lactam ring << < accessible c. Extended-spectrum P Gr+ & Gr Some: have -lactamase inhibitors
2.
Bacterial resistance
-lactamases
4.
Cephalosporins
Classification
a. b. c. d. First-generation : Oral & injectible form Second-generation : mostly injectible Third-generation: mostly injectible Fourth-generation: mostly injectible
c. Nitromidazoles, Metronidazole.
Spectrum: T. vaginalis, G. lamblia, E. histolytica, obligate anaerobic. Toxicity: mutagenic
2. RNA synthesis inhibitors Rifampicin : M. tbc, M. leprae, Legionella, meningitis (N. meningitidis, H. influenzae)
B. Mutational resistance
related to chromosomal mutation unable to interact with antibacterial.
resistance to tetracyclin as a result of transportation into cell electron transport to oxygen this agents are not effective to anaerobic bacteria or to facultative organisms in anaerobic enviroment (e.g. abscess)
E. Active exclusion of the antimicrobial agent from the bacteria. R to tetracyclin is mediated by the
synthesis new transport proteins that actively exluded the drug.
ANTIBACTERIAL
Penicillin Cephalosporin Sulfonamides Aminoglycosides
RESISTANCE MECHANISMS
& Enzymatic inactivation (-lactamase) Target modification (PBPs) Tolerance Active exclusion Target alternation Enzymatic inactivation (acetyltrans-ferase, phosphorilase, nucleotidases) Target alteration (30S ribosomal unit) Decriaced cell wall permiability Active exclusion protein) (mutation of membrane transport
Quinolones