Majmaa University Preparatory Year Program Colleges of Medicine and Dentistry 1st SEMESTER 1431/1432 H

Course Title:

Human Biology
For Premedical Student

1- Lab Safety and rules

By

Dr. Alaa-Eldin Salah-Eldin

Assistant Professor of Cellular and Molecular Biology

Why is Lab Safety Important?

Lab safety is a major aspect of every lab based science class. Lab safety rules and symbols are needed so that students do not injure themselves or their classmates.

Lab Safety Rules

1. Always follow the teachers directions and only do lab work when a teacher is present. 2. Conduct yourself in a responsible manner at all times. No Horseplay! 3. Do not touch any equipment, chemicals, or other materials until told to do so. 4. Do not eat food, drink beverages, or chew gum in the lab. Do not use lab glassware as food or beverage containers 5. Report ALL accidents to your teacher immediately, even if you think it is minor.

Clothing
Wear:
Goggles/safety glasses Closed-toed shoes Clothing made of natural based fiber such as cotton Tie back long hair

Do NOT Wear:
Sandals Jewelry Loose or Baggy clothing

Lab Safety Equipment

Safety Shower

Eye Wash

Safety Goggles

Lab Safety Equipment

Fire Extinguisher Fire Blanket
Fume Hood

Lab Safety Symbols

Found in your textbook

Glassware Heat Eye and Face Sharps

Electrical
Animal

Chemical
Fire

Other Chemical Hazards and Symbols

Flammable

Irritant

Explosive

Corrosive

Toxic/Poison

Environmenta l

Chemical Hazard Symbols and Definitions

Flammable Any substance that will burn if exposed to an open flame.

Explosive A substance that explode if exposed to heat or flame.

may

Chemical Hazard Symbols and Definitions

Toxic/Poison A substance that can lead to death if inhaled, ingested, or absorbed by the skin. Corrosive A substance that can destroy or burn living tissue and can eat away at other materials.

Chemical Hazard Symbols and Definitions

Irritant - A substance that causes inflammation upon contact with skin or mucous membranes. Environmental - Substances that are harmful to the environment. They must be disposed of properly, not washed down the drain.

Accidents and Injuries

Report ALL accidents and injuries to your teacher immediately!!! Be aware of safety hazards associated with each chemical you use. Eye accident Wash the eye with copious amounts of water for at least 15 min. Fire Burns Stop, Drop, and be covered with a fire blanket or soaked with water. Do not remove clothing stuck to victim. Get medical attention immediately.

Waste Disposal
First ask your teacher where/how to dispose of waste. Never pour anything down the drain unless

you are told to do so.

A waste bottle will be supplied for substance that cannot go down the drain

Reasons for proper waste disposal

Hazardous chemicals down the drain can lead to pollution of ground water, lakes, rivers, etc. Plants and animals will die if they are exposed to

hazardous chemical waste.

Serious health problems will become present in people if hazardous waste finds its way into drinking water.

Laboratory Rules
No eating or drinking in labs Do not enter stockroom or back halls Clean up after yourself

Laboratory Etiquette
Clean your personal workspace after use
Wipe down benches with disinfectant before and after use.

Ensure that no trash is left behind

No trash in the sinks

Push in chairs

Place lab items back on carts and trays

Learning Center & Open Labs

The LC and open labs are a bonus, not a right, your flexibility is appreciated Hours are posted on LC and lab doors

Hours are subject to change without notice

Some study items may not be available a day or two before an exam Dont wait until the last day to study

Laboratory Safety
1. Laboratory Hygiene 2. Sharps Safety 3. Safety Equipment 4. Fire Safety 5. Chemical Safety

1. Laboratory Hygiene
Scrub hands thoroughly when finished Avoid cross contamination
Do not touch self, faucets, doorknobs, notebooks, pens etc. with gloves on. Keep a pen or two in your drawer for lab use only

Clean and disinfect your workspace

2. Sharps Safety
Sharps include
Razor and scalpel blades Needles and pins Microscope cover slips Broken glass

Place sharps in sharps boxes Broken Glass into glass box (ask for help) NO SHARPS OR GLASS IN THE TRASH

3. Safety Equipment
Do not hesitate to use safety equipment. It is for student use as well as instructor use. Shower and eyewash (15 min. soak) First aid kit Fire extinguisher, Fire blanket, Alarm pulls

4. Fire Safety
Never leave flames unattended Do not use flammables near ignition sources Fire Extinguishers
Pull ring, aim at fires base, squeeze handle, sweep back and forth (evacuate if >1m3)

Fire Blanket Fire alarm pulls

5. Chemical Safety
Wear gloves and glasses where appropriate Follow instructors directions Dispose of waste properly-Do Not pour down the drain

Laboratory Safety Rules

1. Many things happen in the lab, and you can never be sure how your area has been used, or whether it was properly cleaned up. Because of this, and possible contamination from the animals, you should not bring food or drink into the lab with you! Also, don't put anything that's come in contact with the lab table-pens, pencils, fingers, etc. - into your mouth or eyes.

Laboratory Safety Rules

2. Be on time! There are several ways being late can hurt your grade directly, but there are safety considerations as well Lab instructions, including safety items, are gone over at the beginning of the labs.

Laboratory Safety Rules

3. It's always a good idea to look your lab exercise over ahead of time, and make notes of the modifications and warnings given at the beginning of the class.

Laboratory Safety Rules

4. Any time you're not sure, ask!!

Laboratory Safety Rules

5. Wash your hands before and especially after working in the lab.

Laboratory Safety Rules

6. There are drawers and coat hooks available keep all unnecessary objects out of your and other people's way.

Laboratory Safety Rules

7. Although labs rarely involve dangerous materials, some can have lasting effects on clothing, so you might want to bring a protective cover-up.

With some procedures, protective eye coverings will be required. If you have long hair or dangling jewelry, keep them out of your working materials.

Laboratory Safety Rules

8. Clean up your stuff! Your area should look at the end of your lab like it did when you started.
When in groups, everyone is responsible for cleanup. Points may be lost if this is not done.

Laboratory Safety Rules

9. When working with chemicals or cultures, make sure you do not mix things together in the source glassware - that includes droppers and mixing rods.
It is not always safe to mix tap water into materials by washing them, either.

Laboratory Safety Rules

10. Dispose of all waste materials properly. The wastebasket is near the door.
Broken glass does not go into the wastebasket there's a special box for it next door in the prep room. Notify the instructor any time something gets broken or if you're injured, even mildly.

Laboratory Safety Rules

11. Try to always stay alert while you're in the lab.
Know where the emergency equipment - first aid kit, fire extinguisher, eyewash fountain, etc are (most are on the front wall or just across the hall). Know the appropriate route out of the building during an emergency.