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General Outline
Drug Stability refers to the capacity of a drug substance or product to remain within established specifications of identity, strength quality, and purity in a specified period of time.
factors that affect drug stability include temperature condition, moisture, light, microbes, packaging materials, transportation, components of drug composition and the nature of the active ingredient.
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Arrhenius Equation
Arrhenius developed a mathematical relationship
where A is the frequency factor, a number that represents the likelihood that collisions would occur with the proper orientation for reaction.
Arrhenius Equation
Taking the natural logarithm of both sides, the equation becomes
y = mx + b
When k is determined experimentally at several temperatures, Ea can be calculated from the slope of a plot of ln k vs. 1/T
Activation Energy
In other words, there is a minimum amount of energy required for reaction: the activation energy, Ea Just as a ball cannot get over a hill if it does not roll up the hill with enough energy, a reaction cannot occur unless the molecules possess sufficient energy to get over the activation energy barrier.
Storage condition of 400C and relative humidity of 75% has been recommended for all the four zones for drug substances and drug products. Studies carried out for 6 months. Accelerated storage conditions must be at least 150C above the expected actual storage temperature and appropriate relative humidity
Drug Kinetics
It is defined as how drug changes with time i.e., study of rate of change. Many drugs are not chemically stable and the principles of chemical kinetics are used to predict the time span for which a drug (pure or formulation) will maintain its therapeutic effectiveness or
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Expiration date
Stability of drugs (t1/2) The half life (t1/2) is defined as the time necessary for a drug to decay by 50% (e.g., From 100% to 50%, 50% to 25%, 20% to 10%)
Shelf life (t0.9)
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Half-Life
Half-life is defined as the time required for one-half of a reactant to react. Because [A] at t1/2 is onehalf of the original [A], [A]t = 0.5 [A]0
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SHELF LIFE
It is defined as the time required for the concentration of the reactant To reduce to 90% of its initial concentration. Shelf life is represented as t90 As per defination the term in equation change to C =90a/100 ; t =t90
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X = independent variable
b = intercept also Ordinate = dependent variable axis abscissa = independent variable axis
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m = slope = Y / X
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Easier to determine parameters (slope and intercept) Simultaneous determination of two parameters (m + b) Logarithms: (a) Common log (base10)
log 100 = log 102 = 2 log 1000 = log 103 = 3
Straight Line:
Slope = m = Y / X = constant
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Curve:
Slope is not constant but function of X Slope = 1st derivative of y with respect to X
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Integration
Determination of area under the curve i.e., sum or amount.
AUC =
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Order of Reactions
Law of mass action The rate of a reaction is proportional to the molar concentrations of the reactants each raised to power equal to the number of molecules undergoing reaction. aA+bB Rate [A]a .[B]b Rate = K [A]a .[B]b Order of reaction = sum of exponents Order of A = a and B = b Then Overall order = a + b
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Product
Example:
The reaction of acetic anhydride with ethyl alcohol to form ethyl acetate and water
(CH3 CO)2 + 2 C2H5OH 2 CH3 CO2 C2H5 + H2O
Rate = K [(CH3 CO)2 O] . [C2H5OH]2 Order for (CH3 CO)2 O is 1st order Order for [C2H5OH]2 is 2nd order Overall order of reaction is 3rd Order
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Fading of dyes ,
Oxidation of vitamin A in an oily solution etc.
- dc/dt =Ko
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Units of the rate constant K: c = co Kt K = co c /t K = Concentration / time = mole / liter . second = M. sec-1
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Determination of t1/2
Let c = co /2 and t1/2 = t substitute in equation; c = co k t t1/2 = co / 2K Note: Rate constant (k) and t1/2 depend on co
Determination of t0.9
Let c = 0.9 co and t= t0.9 substitute in equation; c = co k t t90% = t0.9 = 0.1 co / k
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(b) First order reaction The most common pharmaceutical reactions; e.g; drug absorption & drug degradation The reaction rate of change is proportional to drug concentration i.e. drug conc. is not constant. a [A] k Product (P) Rate = - dc/dt = K [c]1
Equation:
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Lnc
Log co
Logc
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Determination of t1/2
Let t = t1/2 and c = co /2
substitute in ln c = ln co Kt
t1/2 = ln 2/ K = 0.693 / K K units = 0.693 / t1/2 = time-1
Determination of t0.9
Let t = t0.9 c = 0.9 co substitute in ln c = ln co Kt t0.9 = 0.105 / K and K = 0.105/ t0.9
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1 2 3 4 10
100
65.6 43.0 28.19 18.49 1.50
Plot c against t on semi log paper and determine slope, K and t1/2
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Solution:
log 28.195 = 1.45 and log 1.5 = 0.176 slope = 1.45 0.176 / 3 10 = 1.27 / -7 = - 0.181 Equation; log c = log co Kt / 2.303 slope = -K/ 2.303 - 0.181 = - K / 2.303 K = 0.417 min-1 t1/2 = 0.693 / K t1/2 = 0.693 / 0.417 = 1.66 minute
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Special Case
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i.e, K is dependent on initial drug concentration. Half life: Shelf life: t1/2 = 1 / KCo
t0.9 = 0.11 / KCo
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